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Detection of QTL for milk protein percentage in Italian Friesian cattle by AFLP markers and selective genotyping

Published online by Cambridge University Press:  14 August 2008

Elisabetta Milanesi
Affiliation:
Istituto di Zootecnica, Facoltà di Agraria, Università Cattolica del S. Cuore, Piacenza, Italy Sezione di Zootecnica Agraria, Dipartimento di Scienze Animali, Università degli Studi di Milano, Italy
Riccardo Negrini*
Affiliation:
Istituto di Zootecnica, Facoltà di Agraria, Università Cattolica del S. Cuore, Piacenza, Italy
Fausta Schiavini
Affiliation:
Department of Veterinary Sciences and Technologies for Food Safety, Università degli Studi di Milano, Italy
Letizia Nicoloso
Affiliation:
Sezione di Zootecnica Agraria, Dipartimento di Scienze Animali, Università degli Studi di Milano, Italy
Raffaele Mazza
Affiliation:
Unità Latemar, Università Cattolica del S. Cuore, Piacenza, Italy
Fabiola Canavesi
Affiliation:
Associazione Nazionale Allevatori Frisona Italiana, Cremona, Italy
Filippo Miglior
Affiliation:
Agriculture and Agri-Food Canada, Dairy and swine Research and Development Centre, Sherbrooke, Quebec, Canada, J1M 1Z3 Canadian Dairy Network, Guelph, ON, Canada, N1G 4T2
Alessio Valentini
Affiliation:
Department of Animal Productions, Facoltà di Agraria, Università della Tuscia, Viterbo, Italy
Alessandro Bagnato
Affiliation:
Department of Veterinary Sciences and Technologies for Food Safety, Università degli Studi di Milano, Italy
Paolo Ajmone-Marsan
Affiliation:
Sezione di Zootecnica Agraria, Dipartimento di Scienze Animali, Università degli Studi di Milano, Italy
*
*For correspondence; e-mail: riccardo.negrini@unicatt.it

Abstract

We targeted quantitative trait loci (QTL) for milk protein percentage (P%) in two Italian Holstein granddaughter design families using selective genotyping in combination with high throughput amplified fragment length polymorphism (AFLP) markers. A total of 64 extreme high and low sires in respect to estimated breeding value (EBV) for P% (EBVP%) were genotyped with 25 AFLP primer combinations that revealed 305 and 291 polymorphisms in the two families. Association between markers and EBVP% was investigated by a linear model only on bands having paternal origin (105 and 96 AFLP bands in family D and S, respectively). Although no marker was significantly associated with the target trait after correction for multiple comparisons, 17 AFLP markers, significant without correction for multiple tests, were considered suggestive of the presence of a QTL. Eleven of these were successfully located on six Bos taurus (BTA) chromosomes by radiation hybrid or in-silico mapping. Ten of these mapped in the immediate neighbourhood (less than 10 cM) of already described QTL for P%. Suggestive association was verified in four regions by microsatellites analysis: one on BTA 10; one on BTA 28; and two on BTA 18. Microsatellites identified significant effects by single marker and interval mapping analyses on BTA 10 and BTA 28, while they were only suggestive of the presence of QTL on BTA 18. In summary, our results firstly indicate that AFLP markers may be used to seek QTL exploiting a selective genotyping approach in GDD, a wide used experimental design in cattle; secondly, propose two approaches for AFLP mapping, namely in-silico mapping exploiting most updated release from the bovine whole genome sequencing project, and physical mapping exploiting a panel of Bovine/Hamster Radiation Hybrids; and thirdly, provide new information on QTLs for an economic important trait in a never investigated Holstein cattle population. AFLP in combination with selective genotyping can be a useful strategy for QTL searching in minor livestock species, sometimes having large economic impact in marginal areas, where more informative markers are still poorly developed.

Type
Research Article
Copyright
Copyright © Proprietors of Journal of Dairy Research 2008

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References

Ajmone-Marsan, P, Valentini, A, Cassandro, M, Vecchiotti-Antaldi, G, Bertoni, G & Kuiper, M 1997 AFLP™ markers for DNA fingerprinting in cattle. Animal Genetics 28 418426CrossRefGoogle Scholar
Altschul, SF, Gish, W, Miller, W, Myers, EW & Lipman, DJ 1990 Basic local alignment search tool. Journal of Molecular Biology 215 403410CrossRefGoogle ScholarPubMed
Ashwell, MS, Rexroad, CE Jr, Miller, RH, Vanraden, PM & Da, Y 1997 Detection of loci affecting milk production and health traits in an elite US Holstein population using microsatellite markers. Animal Genetics 28 216222CrossRefGoogle Scholar
Bensch, S & Åkesson M 2005 Ten years of AFLP in ecology and evolution: why so few animals? Molecular Ecology 14 28992914CrossRefGoogle ScholarPubMed
Boichard, D, Grohs, C, Bourgeois, F, Cerqueira, F, Faugeras, R, Neau, A, Rupp, R, Amigues, Y, Boscher, MY & Leveziel, H 2003 Detection of genes influencing economic traits in three French dairy cattle breeds. Genetic Selection Evolution 35 77101CrossRefGoogle ScholarPubMed
Darvasi, A & Soller, M 1992 Selective genotyping for determination of linkage between a marker locus and a quantitative trait locus. Theoretical and Applied Genetics 85 353359Google Scholar
Georges, M, Nielsen, D, Mackinnon, M, Mishra, A, Okimoto, R, Pasquino, AT, Sargeant, LS, Sorensen, A, Steele, MR, Zhao, X, Womack, JE & Hoeschele, I 1995 Mapping quantitative trait loci controlling milk production in dairy cattle by exploiting progeny testing. Genetics 139 907920Google ScholarPubMed
de Givry, S, Bouchez, M, Chabrier, P, Milan, D & Schiex, T 2005 CARHTAGENE: multipopulation integrated genetic and radiation hybrid mapping. Bioinformatics 21 17031704CrossRefGoogle Scholar
Heyen, DW, Weller, JI, Ron, M, Band, M, Beever, JE, Feldmesser, E, Da, Y, Wiggans, GR, VanRaden, PM & Lewin, HA 1999 A genome scan for QTL influencing milk production and health traits in dairy cattle. Physiological Genomics 1 165175CrossRefGoogle ScholarPubMed
Hu, Z, Fritz, ER & Reecy, JM 2007 AnimalQTLdb: a livestock QTL database tool set for positional QTL information mining and beyond. Nucleic Acids Research 35 D604609CrossRefGoogle ScholarPubMed
Ihara, N, Takasuga, A, Mizoshita, K, Takeda, H, Sugimoto, M, Mizoguchi, Y, Hirano, T, Itoh, T, Watanabe, T, Reed, KM, Snelling, WM, Kappes, SM, Beattie, CW, Bennet, GL and Sugimoto, Y 2004 A comprehensive genetic map of the cattle genome based on 3802 microsatellites. Genome Research 14 19871998CrossRefGoogle ScholarPubMed
Jann, OC, Aerts, J, Jones, M, Hastings, N, Law, A, McKay, S, Marques, E, Prasad, A, Yu, J, Moore, SS, Floriot, S, Mahe, MF, Eggen, A, Silveri, L, Negrini, R, Milanesi, E, Ajmone-Marsan, P, Valentini, A, Marchitelli, C, Savarese, MC, Janitz, M, Herwig, R, Hennig, S, Gorni, C, Connor, EE, Sonstegard, TS, Smith, T, Drogemuller, C & Williams, JL 2006 A second generation radiation hybrid map to aid the assembly of the bovine genome sequence. BMC Genomics 7 283CrossRefGoogle ScholarPubMed
Khatkar, MS, Thomson, PC, Tammen, I & Raadsma, HW 2004 Quantitative trait loci mapping in dairy cattle: review and meta-analysis. Genetic Selection Evolution 36 163190CrossRefGoogle ScholarPubMed
Mosig, MO, Lipkin, E, Khutoreskaya, G, Tchourzyna, E, Soller, M & Friedmann, A 2001 A whole genome scan for quantitative trait loci affecting milk protein percentage in Israeli-Holstein cattle, by means of selective milk DNA pooling in a daughter design, using an adjusted false discovery rate criterion. Genetics 157 16831698Google Scholar
Muir, BL, Kistemaker, G, Jamrozik, J & Canavesi, F 2007 Genetic parameters for a multiple-trait multiple-lactation random regression test-day model in Italian Holsteins. Journal of Dairy Science 90 15641574CrossRefGoogle ScholarPubMed
Polineni, P, Aragonda, P, Xavier, SR, Furuta, R & Adelson, DL 2006 The bovine QTL viewer: a web accessible database of bovine Quantitative Trait Loci. BMC Bioinformatics 7 283CrossRefGoogle ScholarPubMed
Rodriguez-Zas, SL, Southey, BR, Heyen, DW & Lewin, HA 2002 Interval and composite interval mapping of somatic cell score, yield, and components of milk in dairy cattle. Journal of Dairy Science 85 30813091CrossRefGoogle ScholarPubMed
Ron, M, Feldmesser, E, Golik, M, Tager-Cohen, I, Kliger, D, Reiss, V, Domochovsky, R, Alus, O, Seroussi, E, Ezra, E & Weller, JI 2004 A complete genome scan of the Israeli Holstein population for quantitative trait loci by a daughter design. Journal of Dairy Science 87 476490CrossRefGoogle ScholarPubMed
Samoré, AB, Boettcher, P, Jamrozik, J, Bagnato, A & Groen, A 2002 Genetic parameters for production traits and somatic cell cores estimated with a multiple trait random regression model in Italian Holstein. Proceedings 7th World Congress on Genetics Applied to Livestock Production, Montpellier, France 29 6366Google Scholar
Schiex, T & Gaspin, C 1997 CARTHAGENE: constructing and joining maximum likelihood genetic maps. Proceedings International Conference on Intellegent Systems in Molecular Biology 5 258267Google ScholarPubMed
Schrooten, C, Bovenhuis, H, Coppieters, W & van Arendonk, JAM 2000 Whole genome scan to detect quantitative trait loci for conformation and functional traits in dairy cattle. Journal of Dairy Science 83 795806CrossRefGoogle ScholarPubMed
Sharma, BS, Jansen, GB, Karrow, NA, Kelton, D & Jiang, Z 2006 Detection and characterization of amplified fragment length polymorphism markers for clinical mastitis in Canadian Holsteins. Journal of Dairy Science 89 36533663CrossRefGoogle ScholarPubMed
Seaton, G, Haley, CS, Knott, SA, Kearsey, M & Visscher, PM 2002 QTL Express: mapping quantitative trait loci in simple and complex pedigrees. Bioinformatics 18 339340CrossRefGoogle ScholarPubMed
Viitala, SM, Schulman, NF, de Koning, DJ, Elo, K, Kinos, R, Virta, A, Virta, J, Mäki-Tanila, A & Vilkki, JH 2003 Quantitative trait loci affecting milk production traits in Finnish Ayrshire dairy cattle. Journal of Dairy Science 86 18281836CrossRefGoogle ScholarPubMed
Vos, P, Hogers, R, Bleeker, M, Reijans, M, Van De Lee, T, Hornes, M, Fritjers, A, Pot, J, Peleman, J, Kuiper, M & Zabeau, M 1995 AFLP: a new technique for DNA fingerprinting. Nucleic Acids Research 23 44074414CrossRefGoogle ScholarPubMed
Weller, JI, Kashi, Y & Soller, M 1990 Daughter and granddaughter designs for mapping of quantitative trait loci in dairy cattle. Journal of Dairy Science 73 25252537CrossRefGoogle ScholarPubMed
Williams, JL, Eggen, A, Ferretti, L, Farr, CJ, Gautier, M, Amati, G, Ball, G, Caramorr, T, Critcher, R, Costa, S, Hextall, P, Hills, D, Jeulin, A, Kiguwa, SL, Ross, O, Smith, AL, Saunier, K, Urquhart, B & Waddington, D 2002 A bovine whole-genome radiation hybrid panel and outline map. Mammalian Genome 13 469474CrossRefGoogle ScholarPubMed
Wimmers, K, Murani, E, Ponsuksili, S, Yerle, M & Schellander, K 2002 Detection of quantitative trait loci for carcass traits in the pig by using AFLP. Mammalian Genome 13 206210CrossRefGoogle ScholarPubMed
Xiao, Q, Wibowo, TA, Wu, X, Michal, JJ, Reeves, JJ, Busboom, JR, Thorgaard, GH & Jiang, Z 2007 A simplified QTL mapping approach for screening and mapping of novel AFLP markers associated with beef marbling. Journal of Biotechnology 27 177187CrossRefGoogle Scholar
Zhang, Q, Boichard, D, Hoeschele, I, Ernst, C, Eggen, A, Murkve, B, Pfister-Genskow, M, Witte, LA, Grignola, FE, Uimari, P, Thaller, G & Bishop, MD 1998 Mapping quantitative trait loci for milk production and health of dairy cattle in a large outbred pedigree. Genetics 149 19591973Google Scholar
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