The prediction of silage feeding value from the analysis of herbage at ensiling and the effects of rate
of nitrogen (N) fertilizer, date of harvest and treatment additive at ensiling on fermentation, dry
matter (DM) recovery and effluent production were evaluated in a factorial experiment. Herbage was
harvested from 75 plots, laid out in three replicate blocks of 25, from the primary growth of a
predominantly perennial ryegrass sward. The plots received 72, 96, 120, 144 or 168 kg N/ha and were
harvested on 10, 17, 24 or 31 May or 7 June. Herbage was ensiled for 176 days in laboratory silos
(6 kg capacity), either untreated or treated with formic acid or a bacterial inoculant, each applied at
3 ml/kg herbage. Increasing the rate of N fertilizer and delaying harvest date increased herbage DM
yield but decreased silage digestible organic matter digestibility (D-value) and intake potential
(P < 0·001).
Increasing the rate of N fertilizer increased crude protein (CP), acid insoluble N (AIN) (P < 0·001),
acid detergent fibre (ADF) (P < 0·01) and acid detergent lignin (ADL) concentrations but had no
statistically significant effect (P > 0·05) on pH or the concentrations of ammonia N, lactic or volatile
fatty acids. Delaying harvesting date decreased CP, AIN and ADF concentrations (P < 0·001) and
effluent DM losses (P < 0·001). Treatment with either formic acid or inoculant decreased silage pH
and the concentrations of ammonia N, acetic acid and propanol and increased AIN, ethanol, neutral
detergent fibre (NDF), ADF and cellulose concentrations, effluent volume, DM losses and intake
potential. Formic acid treatment decreased D-value (P < 0·001), whereas inoculant treatment was not
statistically significant (P > 0·05). For potential silage and metabolizable energy (ME) intakes and D-
value, strong positive correlations (P < 0·001) were identified with herbage pH, buffering capacity
and concentrations of CP, AIN, true protein nitrogen (TP), while negative correlations (P < 0·001)
were identified with herbage yield and concentrations of NDF and hemicellulose. With untreated
silages, strong positive correlations (P < 0·001) were identified between silage pH and herbage pH
and concentrations of AIN, buffering capacity, ash, TP and negative correlations (P < 0·001) were
identified with herbage DM yield and concentrations of DM and WSC. Herbage yield and
concentrations of NDF, TP, DM and nitrate N provided the best linear relationship for predicting
potential ME intake of the resultant untreated silages (R2 = 0·94). It is concluded that delaying
harvest date had the most detrimental effect on silage feeding value, although this effect was partially
overcome by reducing the rate of fertilizer N and use of either an inoculant or formic acid additive.
Silage feed value was also highly correlated with the protein and fibre fractions, ash, buffering
capacity and pH of the herbage at ensiling.