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Marker-assisted stacking of null Kunitz trypsin inhibitor and off-flavour generating lipoxygenase-2 in soybean

Published online by Cambridge University Press:  01 July 2021

V. Kumar Open the ORCID record for V. Kumar [Opens in a new window] ,
A. Rani ,
A. K. Anshu  and
T. Tayalkar
V. Kumar*
Affiliation:
ICAR-Indian Institute of Soybean Research, Indore, Madhya Pradesh, India
A. Rani
Affiliation:
ICAR-Indian Institute of Soybean Research, Indore, Madhya Pradesh, India
A. K. Anshu
Affiliation:
ICAR-Indian Institute of Soybean Research, Indore, Madhya Pradesh, India
T. Tayalkar
Affiliation:
ICAR-Indian Institute of Soybean Research, Indore, Madhya Pradesh, India
*
Author for correspondence: V. Kumar, E-mail: Vineet.Kumar@icar.gov.in
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Abstract

Soybean varieties genetically free from Kunitz trypsin inhibitor (KTI) and lipoxygenase-2 (Lox2) are desirable to increase human consumption, as the former is an antinutritional factor that affects protein digestibility while the latter is a principal contributor to off-flavour. In the present investigation, soybean genotypes free from both these undesirable components were developed by introgression of null allele of Lox2 from NRC109 (lox2lox2) into two KTI-free soybean genotypes derived from genotypes JS97-52 and NRC7. Foreground selection of plants in F1, F2, BC1F1, BC1F2, BC2F1 and BC2F2 generations developed from two cross combinations i.e. NRC7-derived KTI-free genotype (N7KTIF)×NRC109 (parental combination 1) and JS97-52 derived KTI free genotype (JKTIF)×NRC109 (parental combination 2) was performed using null allele specific markers and tightly linked simple sequence repeat (SSR) markers for both KTI and Lox2 genes for the identification of homozygous recessive (titilox2lox2) plants. Background selection was performed using 239 and 241 polymorphic SSR markers across the genome. This resulted in the development of 9 and 13 soybean lines stacked for null alleles of both KTI and Lox2 (titilox2lox2) exhibiting recurrent parent genome content more than 97 and 96%, respectively. Days-to-flowering, days-to-maturity, 100-seed weight and yield per plant of the stacked lines developed from both the parental combinations were at par with the respective recurrent parents.

Keywords

Background selectionforeground selectionintrogressionSSR marker
Type
Crops and Soils Research Paper
Information
The Journal of Agricultural Science , Volume 159 , Issue 3-4 , April 2021 , pp. 272 - 280
Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press

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References

Alamu, EO, Popoola, I and Maziya-Dixon, B (2018) Effect of soybean (Glycine max (L.) Merr.) flour inclusion on the nutritional properties and consumer preference of fritters for improved household nutrition. Food Science and Nutrition 6, 1811–1816.10.1002/fsn3.751CrossRefGoogle ScholarPubMed
Berloo, RV (2008) GGT 2.0: versatile software for visualisation and analysis of genetic data. Journal of Heredity 99, 232–236.10.1093/jhered/esm109CrossRefGoogle ScholarPubMed
Davies, CS, Nielsen, SS and Nielsen, NC (1987) Flavor improvement of soybean preparations by genetic removal of lipoxygenase-2. Journal of the American Oil Chemists’ Society 64, 1428–1433.10.1007/BF02636994CrossRefGoogle Scholar
Doyle, JJ and Doyle, JL (1990) Isolation of plant DNA from the fresh tissue. Focus 12, 13–15.Google Scholar
Fukushige, H, Wang, C, Simpson, TD, Gardner, HW and Hildebrand, DF (2005) Purification and identification of linoleic acid hydroperoxides generated by soybean seed lipoxygenases 2 and 3. Journal of Agriculture and Food Chemistry 53, 5691–5694.CrossRefGoogle ScholarPubMed
Hertzler, SR, Lieblein-Boff, JC, Weiler, M and Allgeier, C (2020) Plant proteins: assessing their nutritional quality and effects on health and physical function. Nutrients 12, 3704.CrossRefGoogle ScholarPubMed
ICAR-Indian Institute of Soybean Research (2018) Variety list. Available at https://iisrindore.icar.gov.in/varieties.html, 30 January 2021.Google Scholar
Kang, GY, Choi, SW, Chae, WG and Chung, JII (2020) Accumulation of triple recessive alleles for three antinutritional proteins in soybean with black seed coat and green cotyledon. Journal of Plant Biotechnology 47, 118–123.10.5010/JPB.2020.47.2.118CrossRefGoogle Scholar
Kumar, V, Rani, A and Rawal, R (2013 a) Deployment of gene specific marker in development of Kunitz trypsin inhibitor free soybean genotypes. Indian Journal of Experimental Biology 51, 1125–1129.Google ScholarPubMed
Kumar, V, Rani, A, Rawal, R and Husain, SM (2013 b) Lipoxygenase-2-free Indian soybean (Glycine max L.) genotypes. Current Science 104, 586–587.Google Scholar
Kumar, V, Rani, A and Rawal, R (2014) Identification of simple sequence repeat markers tightly linked to lipoxygenase-2 gene in soybean. Indian Journal of Biotechnology 13, 455–458.Google Scholar
Kumar, V, Rani, A, Rawal, R and Mourya, V (2015) Marker assisted accelerated introgression of null allele of Kunitz trypsin inhibitor in soybean. Breeding Science 65, 447–452.CrossRefGoogle ScholarPubMed
Lee, KJ, Hwang, JE, Velusamy, V, Ha, BK, Kim, JB, Kim, SH, Ahn, JW, Kang, SY and Kim, DS (2014) Selection and molecular characterization of a lipoxygenase-free soybean mutant line induced by gamma irradiation. Theoretical and Applied Genetics 127, 2405–2413.CrossRefGoogle ScholarPubMed
Moraes, RMAD, Soares, TCB, Colombo, LR, Salla, MFS, de Almeida Barros, JGND, Piovesan, EG, de Barros, EG and Moreira, MA (2006) Assisted selection by specific DNA markers for genetic elimination of the Kunitz trypsin inhibitor and lectin in soybean seeds. Euphytica 149, 221–226.CrossRefGoogle Scholar
Omar, A, Kalra, RS, Putri, J, Elwakeel, A, Kaul, SC and Wadhwa, R (2020) Soyasapogenol-A targets CARF and results in suppression of tumor growth and metastasis in p53 compromised cancer cells. Scientific reports 10, 6323.10.1038/s41598-020-62953-5CrossRefGoogle ScholarPubMed
Orman, A, Johnson, DL, Comander, A and Brockton, N (2020) Breast cancer: a lifestyle medicine approach. American Journal of Lifestyle Medicine 14, 483–494.10.1177/1559827620913263CrossRefGoogle ScholarPubMed
Pabich, M and Materska, M (2019) Biological effect of soy isoflavones in the prevention of civilization diseases. Nutrients 11, 1660.CrossRefGoogle ScholarPubMed
Rani, A and Kumar, V (2015) Development and commercialization of Kunitz trypsin inhibitor-free Indian soybean (Glycine max L.) genotypes. Current Science 109, 855–856.Google Scholar
Rani, A and Kumar, V (2016) NRC-109 (IC0612435; INGR15017), a Soybean (Glycine max L. Merril) for lipoxygenase-2 free soybean with early maturity (85 days). Indian Journal of Plant Genetic Resources 29, 205–206.Google Scholar
Rani, A, Kumar, V, Mourya, V, Singh, RK and Husain, SM (2011) Validation of SSR markers linked to null Kunitz tryspin inhibitor allele in Indian soybean [Glycine max (L.) Merr.] population. Journal of Plant Biochemistry and Biotechnology 20, 258–261.CrossRefGoogle Scholar
Rani, A, Kumar, V, Shukla, S, Jha, P and Rawal, R (2016) DNA barcoding of Indian soybean varieties as constructed through SSR markers. Seed Science and Technology 44, 357–369.CrossRefGoogle Scholar
Rawal, R, Kumar, V, Rani, A and Gokhale, SM (2020) Genetic elimination of off-flavour generating lipoxygenase-2 gene of soybean through marker assisted backcrossing and its effect on seed longevity. Plant Breeding and Biotechnology 8, 163–173.CrossRefGoogle Scholar
Reinprecht, Y, Luk-Labey, SY, Yu, K, Poysa, VW, Rajcan, I, Ablett, GR and Peter Pauls, K (2011) Molecular basis of seed lipoxygenase null traits in soybean line OX948. Theoretical and Applied Genetics 122, 1247–1264.CrossRefGoogle ScholarPubMed
Rizzo, G (2020) The antioxidant role of soy and soy foods in human health. Antioxidants 9, 635.CrossRefGoogle ScholarPubMed
Shin, JH, Van, K, Kim, KD, Lee, YH, Jun, TH and Lee, SH (2012) Molecular sequence variations of the lipoxygenase-2 gene in soybean. Theoretical and Applied Genetics 124, 613–622.CrossRefGoogle ScholarPubMed
Suda, I, Hajika, M, Nishiba, Y, Furuta, S and Igita, K (1995) Simple and rapid method for the detection of individual lipoxygenase isozymes in soybean. Journal of Agriculture and Food Chemistry 43, 742–747.CrossRefGoogle Scholar
Wang, J, Huaqin, K, Zhang, Z, Yang, Y, Yan, L, Zhang, M, Song, S and Guan, Y (2020) Generation of seed lipoxygenase-free soybean using CRISPR-Cas9. The Crop Journal 8, 432–439.10.1016/j.cj.2019.08.008CrossRefGoogle Scholar
Wilson, LA (1996) Comparison of lipoxygenase-null and lipoxygenase containing soybeans for foods. In Piazza, G (ed.), Lipoxygenase Enzymes and Lipoxygenase Pathway. Champaign, IL, USA: AOCS Press, pp. 209–225.10.1201/9781439831977.ch12CrossRefGoogle Scholar
Zilic, S, Milivojevic, M, Sobajic, S and Maksimovic, M (2006) Effect of multiple alleles on oxidative stability and germination of soybean seeds subsequent to the accelerated ageing test. Genetika 38, 37–48.CrossRefGoogle Scholar
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