Hostname: page-component-8448b6f56d-c4f8m Total loading time: 0 Render date: 2024-04-25T05:36:50.777Z Has data issue: false hasContentIssue false
  • English
  • Français

Germination vigour difference of superior and inferior rice grains revealed by physiological and gene expression studies

Published online by Cambridge University Press:  10 July 2018

Y. F. Zhao ,
H. Z. Sun ,
H. L. Wen ,
Y. X. Du ,
J. Zhang ,
J. Z. Li ,
T. Peng  and
Q. Z. Zhao
Y. F. Zhao
Affiliation:
Collaborative Innovation Center of Henan Grain Crops, Henan Agricultural University, Zhengzhou 450002, China Research Center for Rice Engineering in Henan Province, Henan Agricultural University, Zhengzhou 450002, China Key Laboratory of Rice Biology in Henan Province, Henan Agricultural University, Zhengzhou 450002, China
H. Z. Sun
Affiliation:
Collaborative Innovation Center of Henan Grain Crops, Henan Agricultural University, Zhengzhou 450002, China Research Center for Rice Engineering in Henan Province, Henan Agricultural University, Zhengzhou 450002, China Key Laboratory of Rice Biology in Henan Province, Henan Agricultural University, Zhengzhou 450002, China
H. L. Wen
Affiliation:
Collaborative Innovation Center of Henan Grain Crops, Henan Agricultural University, Zhengzhou 450002, China Research Center for Rice Engineering in Henan Province, Henan Agricultural University, Zhengzhou 450002, China Key Laboratory of Rice Biology in Henan Province, Henan Agricultural University, Zhengzhou 450002, China
Y. X. Du
Affiliation:
Collaborative Innovation Center of Henan Grain Crops, Henan Agricultural University, Zhengzhou 450002, China Research Center for Rice Engineering in Henan Province, Henan Agricultural University, Zhengzhou 450002, China Key Laboratory of Rice Biology in Henan Province, Henan Agricultural University, Zhengzhou 450002, China
J. Zhang
Affiliation:
Collaborative Innovation Center of Henan Grain Crops, Henan Agricultural University, Zhengzhou 450002, China Research Center for Rice Engineering in Henan Province, Henan Agricultural University, Zhengzhou 450002, China Key Laboratory of Rice Biology in Henan Province, Henan Agricultural University, Zhengzhou 450002, China
J. Z. Li
Affiliation:
Collaborative Innovation Center of Henan Grain Crops, Henan Agricultural University, Zhengzhou 450002, China Research Center for Rice Engineering in Henan Province, Henan Agricultural University, Zhengzhou 450002, China Key Laboratory of Rice Biology in Henan Province, Henan Agricultural University, Zhengzhou 450002, China
T. Peng*
Affiliation:
Collaborative Innovation Center of Henan Grain Crops, Henan Agricultural University, Zhengzhou 450002, China Research Center for Rice Engineering in Henan Province, Henan Agricultural University, Zhengzhou 450002, China Key Laboratory of Rice Biology in Henan Province, Henan Agricultural University, Zhengzhou 450002, China
Q. Z. Zhao*
Affiliation:
Collaborative Innovation Center of Henan Grain Crops, Henan Agricultural University, Zhengzhou 450002, China Research Center for Rice Engineering in Henan Province, Henan Agricultural University, Zhengzhou 450002, China Key Laboratory of Rice Biology in Henan Province, Henan Agricultural University, Zhengzhou 450002, China
*
Author for correspondence: T. Peng, E-mail: lypengting@163.com Q. Z. Zhao, E-mail: qzzhaoh@126.com
Author for correspondence: T. Peng, E-mail: lypengting@163.com Q. Z. Zhao, E-mail: qzzhaoh@126.com
Get access Rights & Permissions [Opens in a new window]

Abstract

Superior and inferior rice grains have different weights and are located on the upper primary branch and lower secondary branches of the panicle, respectively. To study differences in germination vigour of these two types of grain, a number of factors were investigated from 0 to 48 h of germination. The present study demonstrated that in inferior grains the starch granule structure was looser at 0 h, with full water absorption at 48 h, while in superior grains the structure was tight and dense. Relative water content increased, and dry matter decreased, more rapidly in inferior grains than in superior ones. Abscisic acid and gibberellin levels, as well as α-amylase activity, also changed more rapidly in inferior grains, while soluble sugar content and amylase coding gene expression increased more rapidly in inferior than superior grains during early germination. The expression of OsGAMYB was higher in inferior grains at 24 h but higher in superior grains at 48 h. The phenotypic index of seedlings was higher in seedlings from superior grains at the two-leaf stage. However, the thousand-grain weight and yield per plant in superior and inferior plants showed no significant difference at harvest. The present study indicates that inferior grains germinate faster than superior ones in the early germination stage. Although inferior grains produced weaker seedlings, it is worthwhile using them in rice production due to their comparative yield potential over that of superior grains.

Keywords

Ricegermination vigourinferior grainssuperior grains
Type
Crops and Soils Research Paper
Information
The Journal of Agricultural Science , Volume 156 , Issue 3 , April 2018 , pp. 350 - 357
Check for updates
Copyright
Copyright © Cambridge University Press 2018 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Appleford, NEJ and Lenton, JR (1997) Hormonal regulation of α-amylase gene expression in germinating wheat (Triticum aestivum) grains. Physiologia Plantarum 100, 534–542.CrossRefGoogle Scholar
Bewley, JD (1997) Seed germination and dormancy. Plant Cell 9, 1055–1066.CrossRefGoogle ScholarPubMed
Bewley, JD (2001) Seed germination and reserve mobilization. Encyclopedia of life science, UK: Nature Publishing Group/www.els.net, pp. 1–7.Google Scholar
Gubler, F, Chandler, PM, White, RG, Llewellyn, DJ and Jacobsen, JV (2002) Gibberellin signaling in barley aleurone cells. Control of SLN1 and GAMYB expression. Plant Physiology 129, 191–200.CrossRefGoogle ScholarPubMed
He, D, Han, C and Yang, P (2011) Gene expression profile changes in germinating rice. Journal of Integrative Plant Biology 53, 835–844.CrossRefGoogle ScholarPubMed
Higgins, TJV, Jacobsen, JV and Zwar, JA (1982) Gibberellic acid and abscisic acid modulate protein synthesis and mRNA levels in barley aleurone layers. Plant Molecular Biology 1, 191–215.CrossRefGoogle ScholarPubMed
Jacobsen, JV and Beach, LR (1985) Control of transcription of α-amylase and rRNA genes in barley aleurone protoplasts by gibberellin and abscisic acid. Nature 316, 275–277.CrossRefGoogle Scholar
Kaneko, M, Itoh, H, Ueguchi-Tanaka, M, Ashikari, M and Matsuoka, M (2002) The alpha-amylase induction in endosperm during rice seed germination is caused by gibberellin synthesized in epithelium. Plant Physiology 128, 1264–1270.CrossRefGoogle ScholarPubMed
Kaneko, M, Inukai, Y, Ueguchi-Tanaka, M, Itoh, H, Izawa, T, Kobayashi, Y, Hattori, T, Miyao, A, Hirochika, H, Ashikari, M and Matsuoka, M (2004) Loss-of-function mutations of the rice GAMYB gene impair alpha-amylase expression in aleurone and flower development. Plant Cell 16, 33–44.CrossRefGoogle ScholarPubMed
Koornneef, M and Van der Veen, JH (1980) Induction and analysis of gibberellin sensitive mutants in Arabidopsis thaliana (L.) Heynh. Theoretical and Applied Genetics 58, 257–263.CrossRefGoogle ScholarPubMed
Koornneef, M, Bentsink, L and Hilhorst, H (2002) Seed dormancy and germination. Current Opinion in Plant Biology 5, 33–36.CrossRefGoogle ScholarPubMed
Kucera, B, Cohn, MA and Leubner-Metzger, G (2005) Plant hormone interactions during seed dormancy release and germination. Seed Science Research 15, 281–307.CrossRefGoogle Scholar
Nambara, E, Okamoto, M, Tatematsu, K, Yano, R, Seo, M and Kamiya, Y (2010) Abscisic acid and the control of seed dormancy and germination. Seed Science Research 20, 55–67.CrossRefGoogle Scholar
Nonogaki, H, Bassel, GW and Bewley, JD (2010) Germination: still a mystery. Plant Science 179, 574–581.CrossRefGoogle Scholar
O'Neill, SD, Kumagai, MH, Majumdar, A, Huang, N, Sutliff, TD and Rodriguez, RL (1990) The alpha-amylase genes in Oryza sativa: characterization of cDNA clones and mRNA expression during seed germination. Molecular and General Genetics 221, 235–244.CrossRefGoogle ScholarPubMed
Park, GG, Park, JJ, Yoon, J, Yu, SN and An, G (2010) A RING finger E3 ligase gene, Oryza sativa Delayed Seed Germination 1 (OsDSG1), controls seed germination and stress responses in rice. Plant Molecular Biology 74, 467–478.CrossRefGoogle ScholarPubMed
Peng, T, Lv, Q, Zhang, J, Li, J, Du, Y and Zhao, Q (2011) Differential expression of the microRNAs in superior and inferior spikelets in rice (Oryza sativa). Journal of Experimental Botany 62, 4943–4954.CrossRefGoogle ScholarPubMed
Peng, T, Du, Y, Zhang, J, Li, J, Liu, Y, Zhao, Y, Sun, H and Zhao, Q (2013) Genome-wide analysis of 24-nt siRNAs dynamic variations during rice superior and inferior grain filling. PloS One 8, e61029. https://doi.org/10.1371/journal.pone.0061029Google ScholarPubMed
Peng, T, Lü, Q, Zhao, Y-F, Sun, H-Z, Han, Y-C, Du, Y-X, Zhang, J, Li, J-Z, Wang, L-L and Zhao, Q-Z (2015) Superior grains determined by grain weight are not fully correlated with the flowering order in rice. Journal of Integrative Agriculture 14, 847–855.CrossRefGoogle Scholar
Perata, P, Matsukura, C, Vernieri, P and Yamaguchi, J (1997) Sugar repression of a gibberellin-dependent signaling pathway in barley embryos. Plant Cell 9, 2197–2208.CrossRefGoogle ScholarPubMed
Rajjou, L, Duval, M, Gallardo, K, Catusse, J, Bally, J, Job, C and Job, D (2012) Seed germination and vigor. Annual Review of Plant Biology 63, 507–533.CrossRefGoogle ScholarPubMed
Seo, M, Nambara, E, Choi, G and Yamaguchi, S (2009) Interaction of light and hormone signals in germinating seeds. Plant Molecular Biology 69, 463–472.CrossRefGoogle ScholarPubMed
Thomas, BR and Rodriguez, RL (1994) Metabolite signals regulate gene expression and source/sink relations in cereal seedlings. Plant Physiology 106, 1235–1239.CrossRefGoogle ScholarPubMed
Umemura, TA, Perata, P, Futsuhara, Y and Yamaguchi, J (1998) Sugar sensing and α-amylase gene repression in rice embryos. Planta 204, 420–428.CrossRefGoogle ScholarPubMed
Yamaguchi, S (2008) Gibberellin metabolism and its regulation. Annual Review of Plant Biology 59, 225–251.CrossRefGoogle ScholarPubMed
Yang, J and Zhang, J (2010) Grain-filling problem in ‘super’ rice. Journal of Experimental Botany 61, 1–5.CrossRefGoogle ScholarPubMed
Zhang, H, Tan, G, Yang, L, Yang, J, Zhang, J and Zhao, B (2009) Hormones in the grains and roots in relation to post-anthesis development of inferior and superior spikelets in japonica/indica hybrid rice. Plant Physiology and Biochemistry 47, 195–204.CrossRefGoogle ScholarPubMed
Zhao, Y and Wang, T (2001) Analysis of the relationship between α-amylase and germinating rate of rice seeds during the process of seed germination. Chinese Bulletin of Botany 18, 226–230.Google Scholar
Zhu, G, Ye, N and Zhang, J (2009) Glucose-induced delay of seed germination in rice is mediated by the suppression of ABA catabolism rather than an enhancement of ABA biosynthesis. Plant and Cell Physiology 50, 644–651.CrossRefGoogle Scholar