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Effects of tributyrin supplementation on short-chain fatty acid concentration, fibrolytic enzyme activity, nutrient digestibility and methanogenesis in adult Small Tail ewes

Published online by Cambridge University Press:  19 June 2018

Q. C. Ren ,
J. J. Xuan ,
Z. Z. Hu ,
L. K. Wang ,
Q. W. Zhan ,
S. F. Dai ,
S. H. Li ,
H. J. Yang ,
W. Zhang  and
L. S. Jiang
Q. C. Ren
Affiliation:
Anhui Science and Technology University, Fengyang 233100, People's Republic of China
J. J. Xuan
Affiliation:
Anhui Science and Technology University, Fengyang 233100, People's Republic of China
Z. Z. Hu
Affiliation:
Anhui Science and Technology University, Fengyang 233100, People's Republic of China
L. K. Wang*
Affiliation:
Anhui Science and Technology University, Fengyang 233100, People's Republic of China
Q. W. Zhan
Affiliation:
Anhui Science and Technology University, Fengyang 233100, People's Republic of China
S. F. Dai
Affiliation:
Anhui Science and Technology University, Fengyang 233100, People's Republic of China
S. H. Li
Affiliation:
Anhui Science and Technology University, Fengyang 233100, People's Republic of China
H. J. Yang
Affiliation:
State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, People's Republic of China
W. Zhang*
Affiliation:
State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, People's Republic of China
L. S. Jiang
Affiliation:
Beijing Key Laboratory of Dairy Cow Nutrition, Beijing University of Agriculture, Beijing 102206, People's Republic of China
*
Author for correspondence: L. K. Wang, E-mail: wanglk@ahstu.edu.cn and W. Zhang, E-mail: wzhang@cau.edu.cn
Author for correspondence: L. K. Wang, E-mail: wanglk@ahstu.edu.cn and W. Zhang, E-mail: wzhang@cau.edu.cn
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Abstract

In vivo and in vitro trials were conducted to assess the effects of tributyrin (TB) supplementation on short-chain fatty acid (SFCA) concentrations, fibrolytic enzyme activity, nutrient digestibility and methanogenesis in adult sheep. Nine 12-month-old ruminally cannulated Small Tail ewes (initial body weight 55 ± 5.0 kg) without pregnancy were used for the in vitro trial. In vitro substrate made to offer TB at 0, 2, 4, 6 and 8 g/kg on a dry matter (DM) basis was incubated by ruminal microbes for 72 h at 39°C. Forty-five adult Small Tail ewes used for the in vivo trial were randomly assigned to five treatments with nine animals each for an 18-d period according to body weight (55 ± 5.0 kg). Total mixed ration fed to ewes was also used to offer TB at 0, 2, 4, 6 and 8 g/kg on a DM basis. The in vitro trial showed that TB supplementation linearly increased apparent digestibility of DM, crude protein, neutral detergent fibre and acid detergent fibre, and enhanced gas production and methane emissions. The in vivo trial showed that TB supplementation decreased DM intake, but enhanced ruminal fermentation efficiency. Both in vitro and in vivo trials showed that TB supplementation enhanced total SFCA concentrations and carboxymethyl cellulase activity. The results indicate that TB supplementation might exert advantage effects on rumen microbial metabolism, despite having an enhancing effect on methanogenesis.

Keywords

Adult ewefibrolytic enzymemethanogenesisshort-chain fatty acidtributyrin
Type
Animal Research Paper
Information
The Journal of Agricultural Science , Volume 156 , Issue 3 , April 2018 , pp. 465 - 470
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Copyright
Copyright © Cambridge University Press 2018 

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