The influence of dairy cattle feed composition on the manure composition and on the dynamics and plant availability of cattle slurry N was studied. Dairy cows were fed seven different forages either with or without supplemental concentrates. The concentration of N in faeces dry matter varied from 18 to 38 g/kg dry matter and increased with increasing digestibility of the feed. Cattle slurries consisting of a mixture of 0·5 faecal N and 0·5 urinary N were stored according to common agricultural practice in Northern Europe. The mineralization of faecal N during slurry storage was very variable (0·09–0·50). The plant availability of N in the slurries originating from cattle fed with known diets was tested in small, framed field plots with spring barley, under conditions with minimal N losses. The nitrogen uptake in barley was determined and the mineral fertilizer equivalent (MFE) of slurry N was calculated. The net release of mineral N and CO2 from the slurries in soil was also measured in a parallel incubation study. The MFE of cattle slurry N varied from 53 to 75%. After correcting for the measured urine-N/faeces-N ratio and expected ammonia emission, the MFE varied from 51 to 78%. The plant availability and net release of cattle slurry N were influenced by forage type and feeding level. The MFE was negatively correlated with the concentration of crude fibre and neutral detergent fibre (NDF) in the diet, and positively correlated with the dietary protein content. The net release of CO2 from the slurries after 12 weeks in soil was significantly influenced by the concentration of crude fibre in the diet. The plant availability of slurry N was significantly correlated with the ammonium content (R2=0·53) and negatively correlated with the slurry C[ratio ]N ratio (R2=0·67) and the dry matter[ratio ]N ratio (R2=0·58). Residual slurry N left in the soil after harvest of the first crop varied from 0·25 to 0·47 of total slurry N. It is concluded that the fibre and the protein content of cattle diets have a significant influence on the plant availability of cattle slurry N and on the amount of residual slurry N remaining in the soil after the first growing season.