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Culture and fertilization of sheep ovarian oocytes:III. Evidence on fertilization in the sheep oviduct based on pronucleate and cleaved eggs

Published online by Cambridge University Press:  27 March 2009

J. F. Quirke  and
I. Gordon
J. F. Quirke
Affiliation:
Faculty of Agriculture, University College, Dublin
I. Gordon
Affiliation:
Faculty of Agriculture, University College, Dublin
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Summary

Four hundred and fifteen oocytes cultured in growth medium for 24·5–28·5 h were transferred to the oviducts of 23 oestrous ewes. Twenty ewes were bred prior to transfer; three remained unbred as a control. After intervals of 24–73 h oocytes were recovered. In 179 oocytes from 19 bred recipients, 19 pronucleateand 26 cleaved eggs were recovered; one apparent 4-cell egg was found in 37 oocytes recovered from unbred ewes.

Type
Research Article
Information
The Journal of Agricultural Science , Volume 76 , Issue 3 , June 1971 , pp. 375 - 377
Copyright
Copyright © Cambridge University Press 1971

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References

REFERENCES

Crosby, T. F. & Gordon, I., (1971). Culture and fertilisation of sheep ovarian oocytes. II. Timing of nuclear maturation in oocytes cultured in growth medium. J. agric. Sci., Camb. 76, 373–4.CrossRefGoogle Scholar
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Quirke, J. F. & Gordon, I. (1971). Culture and fertilisation of sheep ovarian oocytes. I. Effect of culture medium on resumption of meiosis. J. agric. Sci., Camb. 76, 369–72.CrossRefGoogle Scholar