Skip to main content Accessibility help
×
Home
Hostname: page-component-55597f9d44-mzfmx Total loading time: 0.257 Render date: 2022-08-16T22:14:24.858Z Has data issue: true Feature Flags: { "shouldUseShareProductTool": true, "shouldUseHypothesis": true, "isUnsiloEnabled": true, "useRatesEcommerce": false, "useNewApi": true } hasContentIssue true

Saline–alkaline resistance analysis of rice overexpressing the CsCYP1A gene of alkaline Chlorella

Published online by Cambridge University Press:  27 April 2020

Jiali Liu
Affiliation:
Key Laboratory of Saline-alkali Vegetation Ecology Restoration, Ministry of Education, College of Life Sciences, Northeast Forestry University, No. 26, Hexing Road, Xiangfang District, Harbin, Heilongjiang150040, China
Mingliang He
Affiliation:
Northeast Institute of Geography and Agricultural Ecology of the Chinese Academy of Sciences, HarbinHeilongjiang, 150081, China University of the Chinese Academy of Sciences, Beijing100049, China
Chenxi Liu
Affiliation:
Key Laboratory of Saline-alkali Vegetation Ecology Restoration, Ministry of Education, College of Life Sciences, Northeast Forestry University, No. 26, Hexing Road, Xiangfang District, Harbin, Heilongjiang150040, China
Xu Liao
Affiliation:
Key Laboratory of Saline-alkali Vegetation Ecology Restoration, Ministry of Education, College of Life Sciences, Northeast Forestry University, No. 26, Hexing Road, Xiangfang District, Harbin, Heilongjiang150040, China
Xiufeng Li*
Affiliation:
Northeast Institute of Geography and Agricultural Ecology of the Chinese Academy of Sciences, HarbinHeilongjiang, 150081, China University of the Chinese Academy of Sciences, Beijing100049, China
Ling Wang*
Affiliation:
College of Landscape Architecture, Northeast Forestry University, No. 26, Hexing Road, Xiangfang District, Harbin, Heilongjiang150040, China
Qingjie Guan*
Affiliation:
Key Laboratory of Saline-alkali Vegetation Ecology Restoration, Ministry of Education, College of Life Sciences, Northeast Forestry University, No. 26, Hexing Road, Xiangfang District, Harbin, Heilongjiang150040, China
*
Authors for correspondence: Ling Wang, E-mail: wanglinghlj@126.com; Qingjie Guan, E-mail: guanqingjie@nefu.edu.cn
Authors for correspondence: Ling Wang, E-mail: wanglinghlj@126.com; Qingjie Guan, E-mail: guanqingjie@nefu.edu.cn
Authors for correspondence: Ling Wang, E-mail: wanglinghlj@126.com; Qingjie Guan, E-mail: guanqingjie@nefu.edu.cn

Abstract

Plant cyclophilin (CYP) is related to chloroplast photoprotection, redox and other growth and developmental regulatory processes and responds to stress and improves tolerance to saline–alkali stress. Besides, it exerts peptidyl-prolyl cis/trans isomerase activity to participate in protein synthesis and folding. In this study, Northern blot was used to analyse the CsCYP1A gene (a CYP gene isolated from a Chlorella species) (accession number: KY207381) of tolerant Chlorella vulgaris in soda saline–alkali soil. The result showed that the expression of the CsCYP1A gene was induced by NaHCO3, NaCl and sorbitol. Additionally, Agrobacterium tumefaciens was used to infect the callus of rice (Oryza sativa var. Longjing11) for genetic transformation. Molecular detection confirmed that transgenic seedlings overexpressing CsCYP1A were obtained by hygromycin selection. Treatment with NaCl and NaHCO3 at the five-leaf stage was performed in the seedlings, and the results showed that there were significant differences between the CsCYP1A overexpressed rice lines and non-transgenic (NT) Longjing11 in terms of plant height, fresh weight, chlorophyll content, malondialdehyde content and ascorbate peroxidase activity. The CsCYP1A overexpression rice lines had higher tolerance to NaCl and NaHCO3 than NT. The current findings indicate that CsCYP1A can enhance the tolerance of rice to saline–alkali stress possibly through its involvement in reactive oxygen scavenging pathways.

Type
Crops and Soils Research Paper
Copyright
Copyright © Cambridge University Press 2020

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Baxter, A, Mittler, R and Suzuki, N (2014) ROS as key players in plant stress signaling. Journal of Experimental Botany 65, 12291240.CrossRefGoogle Scholar
Campos, BM, Sforça, ML, Ambrosio, AL, Domingues, MN, Souza, TA, Barbosa, JA, Leme, AF, Perez, CA, Whittaker, SB, Murakami, MT, Zeri, AC and Benedetti, CE (2013) A redox 2-Cys mechanism regulates the catalytic activity of divergent cyclophilins. Plant Physiology 162, 13111323.CrossRefGoogle ScholarPubMed
Dominguez-Solis, JR, He, Z, Lima, A, Ting, J, Buchanan, BB and Luan, S (2008) A cyclophilin links redox and light signals to cysteine biosynthesis and stress responses in chloroplasts. Proceedings of the National Academy of Sciences 105, 1638616391.CrossRefGoogle ScholarPubMed
Gasser, CS, Gunning, DA, Budelier, KA and Brown, SM (1990) Structure and expression of cytosolic cyclophilin/peptidyl-prolyl cistrans isomerase of higher plants and production of active tomato cyclophilin in Escherichia coli. Proceedings of the National Academy of Sciences 87, 95199523.CrossRefGoogle ScholarPubMed
Giavalisco, P, Kapitza, K, Kolasa, A, Buhtz, A and Kehr, J (2006) Towards the proteome of Brassica napus phloem sap. Proteomics 6, 896909.CrossRefGoogle ScholarPubMed
Godoy, AV, Lazzaro, AS, Casalongué, CA and Segundob, BS (2000) Expression of a solanum tuberosum cyclophilin gene is regulated by fungal infection and abiotic stress conditions. Plant Science 152, 123134.CrossRefGoogle Scholar
Jiang, M, Yang, W, Jiang, X and Chen, QY (1994) Active oxygen damage effect of chlorophyll degradation in rice seedlings under osmotic stress. Acta Botanica Sinica 36, 289295.Google Scholar
Kaur, G, Singh, S, Dutta, T, Kaur, H, Singh, B, Pareek, A and Singh, P (2016) The peptidyl-prolyl cis–trans isomerase activity of the wheat cyclophilin, TaCYPA-1, is essential for inducing thermotolerance in Escherichia coli. Biochimie Open 2, 915.CrossRefGoogle ScholarPubMed
Kumari, S, Joshi, R, Singh, K, Roy, S, Tripathi, AK, Singh, P, Singla-Pareek, SL and Pareek, A (2015) Expression of a cyclophilin OsCYP2-P isolated from a salt-tolerant landrace of rice in tobacco alleviates stress via ion homeostasis and limiting ROS accumulation. Functional & Integrative Genomics 15, 395412.CrossRefGoogle ScholarPubMed
Lee, SS, Park, HJ, Yoon, DH, Kim, BG, Ahn, JC and Luan, S (2015) Rice cyclophilin OsCYP18-2 is translocated to the nucleus by an interaction with skip and enhances drought tolerance in rice and arabidopsis. Plant, Cell & Environment 38, 20712087.CrossRefGoogle ScholarPubMed
Lee, A, Lee, SS, Won, YJ, Park, HJ, Lim, BR, Kim, HS, Ahn, JC and Cho, HS (2016) The OsCYP19-4 gene is expressed as multiple alternatively spliced transcripts encoding isoforms with distinct cellular localizations and PPIase activities under cold stress. International Journal of Molecular Sciences 17, 1154.CrossRefGoogle ScholarPubMed
Liao, X, He, ML, Zhang, SY, Ma, HY, Wang, XH, Luo, QX and Guan, QJ (2017) Prokaryotic expression, activity assay of Chlorella cyclophilin A and salt tolerance analysis of arabidopsis over-expressing CsCYP1A. Bulletin of Botanical Research 37, 722729.Google Scholar
Lu, T, Lu, G, Fan, D, Zhu, C, Li, W, Zhao, Q, Feng, Q, Zhao, Y, Guo, Y, Li, W, Huang, X and Han, B (2010) Function annotation of the rice transcriptome at single-nucleotide resolution by RNA-seq. Genome Research 20, 12381249.CrossRefGoogle ScholarPubMed
Mainali, HR, Chapman, P and Dhaubhadel, S (2014) Genome-wide analysis of cyclophilin gene family in soybean (Glycine max). BMC Plant Biology 14, 111.CrossRefGoogle Scholar
Nuc, K, Nuc, P and Słomski, R (2001) Yellow lupine cyclophilin transcripts are highly accumulated in the nodule meristem zone. Molecular Plant–Microbe Interactions 14, 13841394.CrossRefGoogle ScholarPubMed
Qiao, K, Takano, T and Liu, S (2015) Discovery of two novels highly tolerant NaHCO3 trebouxiophytes: identification and characterization of microalgae from extreme saline-alkali soil. Algal Research 9, 245253.CrossRefGoogle Scholar
Ruan, SL, Ma, HS, Wang, SH, Fu, YP, Xin, Y and Liu, WZ (2011) Proteomic identification of OsCYP2, a rice cyclophilin that confers salt tolerance in rice (Oryza sativa L.) seedlings when overexpressed. BMC Plant Biology 10, 11861471.Google Scholar
Sato, Y, Murakami, T, Funatsuki, H, Matsuba, S, Saruyama, H and Tanida, M (2001) Heat shock-mediated APX gene expression and protection against chilling injury in rice seedlings. Journal of Experimental Botany 52, 145151.CrossRefGoogle ScholarPubMed
Sirpiö, S, Khrouchtchova, A, Allahverdiyeva, Y, Hansson, M, Fristedt, R and Vener, AV (2008) AtCYP38 ensures early biogenesis, correct assembly and sustenance of photosystem ii. The Plant journal: for cell and molecular biology 55, 639651.CrossRefGoogle ScholarPubMed
Suzuki, N, Koussevitzky, S, Mittler, R and Miller, G (2012) ROS and redox signaling in the response of plants to abiotic stress. Plant, Cell & Environment 35, 259270.CrossRefGoogle ScholarPubMed
Toki, S, Hara, N, Ono, K, Onodera, H, Tagiri, A, Oka, S and Tanaka, H (2006) Early infection of scutellum tissue with Agrobacterium allows high-speed transformation of rice. The Plant Journal 47, 969976.CrossRefGoogle ScholarPubMed
Tong, HS, Wang, SS, Zhu, XN and Ding, WN (2017) Research progress of plant cyclophilin gene functions. Acta Botanica Boreali-Occidentalia Sinica 37, 830838.Google Scholar
Trivedi, DK, Yadav, S, Vaid, N and Tuteja, N (2012) Genome wide analysis of, cyclophilin, gene family from rice and arabidopsis and its comparison with yeast. Plant Signaling & Behavior 7, 16531666.CrossRefGoogle ScholarPubMed
Upadhyaya, NM, Surin, B and Ramm, K (2000) Agrobacterium-mediated transformation of Australian rice cultivars jarrah and amaroo using modified promoters and selectable markers. Australian Journal of Plant Physiology 27, 201210.Google Scholar
Wang, BM and Tan, XF (2008) The structure, function and expressive regulation of plant cyclophilin genes. Journal of Central South University of Forestry & Technology 28, 168174.Google Scholar
Yoon, DH, Lee, SS, Park, HJ, Lyu, JI, Chong, WS, Liu, JR, Kim, BG, Ahn, JC and Cho, HS (2016) Overexpression of OsCYP19-4 increases tolerance to cold stress and enhances grain yield in rice (Oryza sativa). Journal of Experimental Botany 67, 6982.CrossRefGoogle Scholar
Yu, XJ, Cao, SY, Dong, YM, Bi, BL, Zhang, YH and Xu, JQ (2016) Research progress of calcium binding proteins in pollen growth and development. Acta Botanica Boreali-Occidentalia Sinica.Google Scholar
1
Cited by

Save article to Kindle

To save this article to your Kindle, first ensure coreplatform@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about saving to your Kindle.

Note you can select to save to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be saved to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

Find out more about the Kindle Personal Document Service.

Saline–alkaline resistance analysis of rice overexpressing the CsCYP1A gene of alkaline Chlorella
Available formats
×

Save article to Dropbox

To save this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you used this feature, you will be asked to authorise Cambridge Core to connect with your Dropbox account. Find out more about saving content to Dropbox.

Saline–alkaline resistance analysis of rice overexpressing the CsCYP1A gene of alkaline Chlorella
Available formats
×

Save article to Google Drive

To save this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you used this feature, you will be asked to authorise Cambridge Core to connect with your Google Drive account. Find out more about saving content to Google Drive.

Saline–alkaline resistance analysis of rice overexpressing the CsCYP1A gene of alkaline Chlorella
Available formats
×
×

Reply to: Submit a response

Please enter your response.

Your details

Please enter a valid email address.

Conflicting interests

Do you have any conflicting interests? *