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Effects of pantothenic acid and folic acid supplementation on total tract digestibility coefficient, ruminal fermentation, microbial enzyme activity, microflora and urinary purine derivatives in dairy bulls

Published online by Cambridge University Press:  22 November 2019

Z. Z. Wu
Affiliation:
College of Animal Sciences and Veterinary Medicines, Shanxi Agricultural University, Taigu, Shanxi030801, P. R. China
C. Wang
Affiliation:
College of Animal Sciences and Veterinary Medicines, Shanxi Agricultural University, Taigu, Shanxi030801, P. R. China
G. W. Zhang
Affiliation:
College of Animal Sciences and Veterinary Medicines, Shanxi Agricultural University, Taigu, Shanxi030801, P. R. China
Q. Liu
Affiliation:
College of Animal Sciences and Veterinary Medicines, Shanxi Agricultural University, Taigu, Shanxi030801, P. R. China
G. Guo
Affiliation:
College of Animal Sciences and Veterinary Medicines, Shanxi Agricultural University, Taigu, Shanxi030801, P. R. China
W. J. Huo
Affiliation:
College of Animal Sciences and Veterinary Medicines, Shanxi Agricultural University, Taigu, Shanxi030801, P. R. China
J. Zhang
Affiliation:
College of Animal Sciences and Veterinary Medicines, Shanxi Agricultural University, Taigu, Shanxi030801, P. R. China
Y. L. Zhang
Affiliation:
College of Animal Sciences and Veterinary Medicines, Shanxi Agricultural University, Taigu, Shanxi030801, P. R. China
C. X. Pei
Affiliation:
College of Animal Sciences and Veterinary Medicines, Shanxi Agricultural University, Taigu, Shanxi030801, P. R. China
S. L. Zhang
Affiliation:
College of Animal Sciences and Veterinary Medicines, Shanxi Agricultural University, Taigu, Shanxi030801, P. R. China
Corresponding
E-mail address:

Abstract

The effects of pantothenic acid (PA) and folic acid (FA) addition on digestibility coefficient, ruminal fermentation and urinary purine derivative (PD) excretion in dairy bulls were evaluated. Eight rumen-cannulated Holstein dairy bulls were allocated to a replicated 4 × 4 Latin square design according to a 2 × 2 factorial arrangement. Diets were supplemented with two levels of FA (0 or 8.0 mg/kg dietary dry matter [DM]) and two of PA (0 or 60 mg/kg DM). The PA × FA interaction was not significant for all variables. Both supplements increased DM intake and average daily gain, but decreased a feed conversion ratio. Digestibility of DM, organic matter, crude protein and neutral detergent fibre increased, but ether extract digestibility was unchanged for both supplements. Digestibility of acid detergent fibre only increased with FA supplementation. For both supplements, ruminal pH and ammonia nitrogen (N) decreased, but total volatile fatty acid (VFA) concentration increased. Acetate proportion only increased with FA supplementation. Propionate proportion decreased for both supplements. Consequently, the acetate to propionate ratio increased. For both supplements, activity of xylanase and pectinase, population of Ruminococcus albus, R. flavefaciens, Fibrobacter succinogenes and Ruminobacter amylophilus and total PD excretion increased. Additionally, activity of carboxymethylcellulase, cellobiase, α-amylase and protease, and population of total bacteria, fungi, protozoa, methanogens, Butyrivibrio fibrisolvens and Prevotella ruminicola increased with FA addition. The results suggested that PA and FA supplementation stimulated ruminal microbial growth and enzyme activity, resulting in an increased digestibility coefficient and ruminal total VFA concentration in dairy bulls.

Type
Animal Research Paper
Copyright
Copyright © Cambridge University Press 2019

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References

Agarwal, N, Kamra, DN, Chaudhary, LC, Agarwal, I, Sahoo, A and Pathak, NN (2002) Microbial status and rumen enzyme profile of crossbred calves fed on different microbial feed additives. Letters in Applied Microbiology 34, 329336.CrossRefGoogle ScholarPubMed
Allen, MS (2000) Effects of diet on short-term regulation of feed intake by lactating dairy cattle. Journal of Dairy Science 83, 15981624.CrossRefGoogle ScholarPubMed
AOAC (2000) Official Methods of Analysis. 17th edn. Arlington, VA, USA: Association of Official Analytical Chemists International.Google Scholar
Bailey, LB and Gregory, JF (1999) Folate metabolism and requirements. Journal of Nutrition 129, 779782.CrossRefGoogle ScholarPubMed
Ball, GFM (2006) Pantothenic acid. In Ball, GFM (ed.), Vitamins in Foods: Analysis, Bioavailability and Stability. Boca Raton, FL, USA: CRC Press, pp. 211219.Google Scholar
Cummins, KA and Papas, AH (1985) Effect of isocarbon 4 and isocarbon 5 volatile fatty acids on microbial protein synthesis and dry matter digestibility in vitro. Journal of Dairy Science 68, 25882595.CrossRefGoogle Scholar
Dijkstra, BJ and Tamminga, S (1995) Simulation of the effects of diet on the contribution of rumen protozoa to degradation of fibre in the rumen. British Journal of Nutrition 74, 617634.CrossRefGoogle ScholarPubMed
Girard, CL, Benchaar, C, Chiquette, J and Desrochers, A (2009) Net flux of nutrients across the rumen wall of lactating dairy cows as influenced by dietary supplements of folic acid. Journal of Dairy Science 92, 61166122.CrossRefGoogle ScholarPubMed
International Atomic Energy Agency (1997) Estimation of Rumen Microbial Protein Production from Purine Derivatives in Urine. IAEA-TECDOC-945. Vienna, Austria: IAEA.Google Scholar
Kolver, ES and DeVeth, MJ (2002) Prediction of ruminal pH from pasture-based diets. Journal of Dairy Science 85, 12551266.CrossRefGoogle ScholarPubMed
Kongmun, P, Wanapat, M, Pakdee, P and Navanukraw, C (2010) Effect of coconut oil and garlic powder on in vitro fermentation using gas production technique. Livestock Science 127, 3844.CrossRefGoogle Scholar
Krause, KM, Combs, DK and Beauchemin, KA (2002) Effects of forage particle size and grain fermentability in midlactation cows. I. Milk production and diet digestibility. Journal of Dairy Science 85, 19361946.CrossRefGoogle ScholarPubMed
La, SK, Li, H, Wang, C, Liu, Q, Guo, G, Huo, WJ, Zhang, YL, Pei, CX and Zhang, SL (2019) Effects of rumen-protected folic acid and dietary protein level on growth performance, ruminal fermentation, nutrient digestibility and hepatic gene expression of dairy calves. Journal of Animal Physiology and Animal Nutrition 103, 10061014.Google ScholarPubMed
Liu, YC and Whitman, WB (2008) Metabolic, phylogenetic, and ecological diversity of the methanogenic archaea. Annals of the New York Academy of Sciences 1125, 171189.CrossRefGoogle ScholarPubMed
Liu, Q, Wang, C, Li, HQ, Guo, G, Huo, WJ, Pei, CX, Zhang, SL and Wang, H (2017) Effects of dietary protein levels and rumen-protected pantothenate on ruminal fermentation, microbial enzyme activity and bacteria population in Blonde d'aquitaine × Simmental beef steers. Animal Feed Science and Technology 232, 3139.CrossRefGoogle Scholar
Liu, Q, Wang, C, Li, HQ, Guo, G, Huo, WJ, Zhang, SL, Zhang, YL, Pei, CX and Wang, H (2018a) Effects of dietary protein level and rumen-protected pantothenate on nutrient digestibility, nitrogen balance, blood metabolites and growth performance in beef calves. Journal of Animal and Feed Sciences 27, 202210.CrossRefGoogle Scholar
Liu, Q, Wang, C, Guo, G, Huo, WJ, Zhang, YL, Pei, CX, Zhang, SL and Wang, H (2018b) Effects of branched-chain volatile fatty acids supplementation on growth performance, ruminal fermentation, nutrient digestibility, hepatic lipid content and gene expression of dairy calves. Animal Feed Science and Technology 237, 2734.CrossRefGoogle Scholar
Newbold, CJ, Lassalas, B and Jouany, JP (1995) The importance of methanogens associated with ciliate protozoa in ruminal methane production in vitro. Letters in Applied Microbiology 21, 230234.CrossRefGoogle ScholarPubMed
Nozière, P, Ortigues-Marty, I, Loncke, C and Sauvant, D (2010) Carbohydrate quantitative digestion and absorption in ruminants: from feed starch and fibre to nutrients available for tissues. Animal: An International Journal of Animal Bioscience 4, 10571074.CrossRefGoogle ScholarPubMed
Ragaller, V, Lebzien, P, Bigalke, W, Südekum, KH, Hüthera, L and Flachowsky, G (2010) Effects of folic acid supplementation to rations differing in the concentrate to roughage ratio on ruminal fermentation, nutrient flow at the duodenum, and on serum and milk variables of dairy cows. Archives of Animal Nutrition 64, 484503.CrossRefGoogle ScholarPubMed
Ragaller, V, Lebzien, P, Sdekum, KH, Hüther, L and Flachowsky, G (2011) Effects of a pantothenic acid supplementation to different rations on ruminal fermentation, nutrient flow at the duodenum, and on blood and milk variables of dairy cows. Journal of Animal Physiology and Animal Nutrition 95, 730743.CrossRefGoogle ScholarPubMed
Sacadura, FC, Robinson, PH, Evans, E and Lordelo, MM (2008) Effects of a ruminally protected B-vitamin supplement on milk yield and composition of lactating dairy cows. Animal Feed Science and Technology 144, 111124.CrossRefGoogle Scholar
SAS (2002) User's Guide: Statistics, Version 9 Edition 2002. Cary, NC, USA: Statistical Analysis Systems Institute.Google Scholar
Schwab, EC, Schwab, CG, Shaver, RD, Girard, CL, Putnam, DE and Whitehouse, NL (2006) Dietary forage and nonfiber carbohydrate contents influence B-vitamin intake, duodenal flow, and apparent ruminal synthesis in lactating dairy cows. Journal of Dairy Science 89, 174187.CrossRefGoogle ScholarPubMed
Slyter, LL and Weaver, JM (1977) Tetrahydrofolate and other growth requirements of certain strains of Ruminococcus flavefaciens. Applied and Environmental Microbiology 33, 363369.CrossRefGoogle ScholarPubMed
Strickland, KC, Hoeferlin, LA, Oleinik, NV, Krupenko, NI and Krupenko, SA (2010) Acyl carrier protein-specific 4′-phosphopantetheinyl transferase activates 10-formyltetrahydrofolate dehydrogenase. Journal of Biological Chemistry 285, 16271633.CrossRefGoogle ScholarPubMed
Trinci, APJ, Davies, DR, Gull, K, Lawrence, MI, Nielsen, BB, Rickers, A and Theodorou, MK (1994) Anaerobic fungi in herbivorous animals. Mycological Research 98, 129152.CrossRefGoogle Scholar
Van Soest, PJ, Robertson, JB and Lewis, BA (1991) Methods for dietary fiber, neutral detergent fiber and nonstarch polysaccharides in relation to animal nutrition. Journal of Dairy Science 74, 35833597.CrossRefGoogle ScholarPubMed
Verbic, J, Chen, XB, MacLeod, NA and Ørkov, ER (1990) Excretion of purine derivatives by ruminants. Effect of microbial nucleic acid infusion on purine derivative excretion by steers. Journal of Agricultural Science, Cambridge 114, 243248.CrossRefGoogle Scholar
Völker, D, Hüther, L, Daş, G and Abel, H (2011) Pantothenic acid supplementation to support rumen microbes? Archives of Animal Nutrition 65, 163173.CrossRefGoogle ScholarPubMed
Wang, C, Wu, XX, Liu, Q, Guo, G, Huo, WJ, Zhang, YL, Pei, CX and Zhang, SL (2019) Effects of folic acid on growth performance, ruminal fermentation, nutrient digestibility and urinary excretion of purine derivatives in post-weaned dairy calves. Archives of Animal Nutrition 73, 1829.CrossRefGoogle ScholarPubMed
Wejdemar, K (1996) Some factors stimulating the growth of Butyrivibrio fibrisolvens TC33 in clarified rumen fluid. Swedish Journal of Agricultural Research 26, 1118.Google Scholar
Wolin, MJ, Miller, TL and Stewart, CS (1997) Microbe-microbe interactions. In Hobson, PN and Stewart, CS (eds), The Rumen Microbial Ecosystem. London, UK: Blackie Academic and Professional, pp. 467491.CrossRefGoogle Scholar
Yu, Z and Morrison, M (2004) Improved extraction of PCR-quality community DNA from digesta and fecal sample. BioTechniques 36, 808812.CrossRefGoogle Scholar

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Effects of pantothenic acid and folic acid supplementation on total tract digestibility coefficient, ruminal fermentation, microbial enzyme activity, microflora and urinary purine derivatives in dairy bulls
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