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Lack of association of neprilysin gene polymorphisms with Alzheimer's disease in a southern Chinese community

Published online by Cambridge University Press:  02 March 2009

Yan Fu*
Guangzhou Psychiatric Hospital, Guangzhou, Guangdong, China
Ai Feng Li
Guangzhou Psychiatric Hospital, Guangzhou, Guangdong, China
Jia Jun Shi
Department of Psychiatry, University of Chicago, Chicago, Illinois, USA
Mou Ni Tang
Guangzhou Psychiatric Hospital, Guangzhou, Guangdong, China
Yang Bo Guo
Guangzhou Psychiatric Hospital, Guangzhou, Guangdong, China
Zhen Huan Zhao
Guangzhou Psychiatric Hospital, Guangzhou, Guangdong, China
Correspondence should be addressed to: Yan Fu, Guangzhou Psychiatric Hospital, Guangzhou, Guangdong, China. Phone: +852 906 26680; Fax: +852 263 65090. Email:


Background: Increasing evidence suggests that neprilysin (NEP) may be the major degrading enzyme of amyloid beta (Aβ) in the brain and the NEP gene has been proposed as a candidate gene for Alzheimer's disease (AD). Association results between the NEP gene and AD are still preliminary. This study investigates the effect of the polymorphisms of −204G/C and 159C/T in the NEP gene on the development of sporadic Alzheimer's disease (SAD) in a southern Chinese community.

Method: 236 sporadic late-onset AD patients were recruited from Guangzhou Psychiatric Hospital in southern China, and 332 healthy elderly controls were enrolled from three old age homes in suburban Guangzhou. NEP and ApoE genotype were determined by PCR–RFLP.

Results: No differences in genotypic and allelic frequencies of −204G/C and 159C/T polymorphisms in NEP were found between AD and control group (for −204G/C genotype: χ2 = 2.34, P > 0.05; for allele: χ2 = 2.31, P > 0.05; for 159C/T genotype: χ2 = 1.34, P > 0.05; for allele: χ2 = 0.88, P > 0.05). Neither was any difference found in genotypic and allelic frequency when stratified by sex or by ApoE ε4 allele (P > 0.05).

Conclusions: Our results suggest that −204G/C and 159C/T polymorphisms of the NEP gene may not be associated with SAD. Moreover, both sex and ApoE ε4 allele do not affect the distribution of NEP gene polymorphisms.

Research Article
Copyright © International Psychogeriatric Association 2009

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