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Heat-initiated prebiotic formation of peptides from glycine/aspartic acid and glycine/valine in aqueous environment and clay suspension

Published online by Cambridge University Press:  14 July 2009

Chandra Kala Pant ,
Hem Lata ,
Hari Datt Pathak  and
Mohan Singh Mehata
Chandra Kala Pant*
Affiliation:
Department of Chemistry, DSB Campus, Kumaun University, Nainital263 002, Uttarakhand, India
Hem Lata
Affiliation:
Department of Chemistry, DSB Campus, Kumaun University, Nainital263 002, Uttarakhand, India
Hari Datt Pathak
Affiliation:
Department of Chemistry, DSB Campus, Kumaun University, Nainital263 002, Uttarakhand, India
Mohan Singh Mehata*
Affiliation:
Photophysics Laboratory, Department of Physics, Kumaun University, Nainital, India; present address RIES, Hokkaido University, Sapporo001-0020, Japan
*
email: ckp_ku@yahoo.co.in
email: msmehata@yahoo.com
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Abstract

The effect of heat on the reaction system of glycine/aspartic acid and glycine/valine in the aqueous environment as well as in montmorillonite clay suspension with or without divalent cations (Ca2+, Mg2+ and Ni2+) has been investigated at 85°C±5°C for varying periods under prebiotic drying and wetting conditions. The resulting products were analysed and characterized by chromatographic and spectroscopic methods. Peptide formation appears to depend on the duration of heat effect, nature of reactant amino acids and, to some extent, on montmorillonite clay incorporated with divalent cations. In the glycine/aspartic acid system, oligomerization of glycine was limited up to trimer level (Gly)3 along with the formation of glycyl-aspartic acid, while linear and cyclic peptides of aspartic acid were not formed, whereas the glycine/valine system preferentially elongated homo-oligopeptide of glycine up to pentamer level (Gly)5 along with formation of hetero-peptides (Gly-Val and Val-Gly). These studies are relevant in the context of the prebiotic origin of proteins and the role of clay and metal ions in condensation and oligomerization of amino acids. The length of the bio-oligomer chain depends upon the reaction conditions. However, condensation into even a small length seems significant, as the same process would have taken millions of years in the primitive era of the Earth, leading to the first proteins.

Keywords

amino acidsmontmorillonite clayoligopeptidesprebiotic chemical evolution
Type
Research Article
Information
International Journal of Astrobiology , Volume 8 , Issue 2 , April 2009 , pp. 107 - 115
Copyright
Copyright © Cambridge University Press 2009

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References

Basiuk, V.A., Gromovoy, T.Y., Golovaty, V.G. & Glukhoy, A.M. (1999). Mechanism of amino acid poly condensation on silica and alumina surfaces. Origins Life Evol. Biosphere 20, 483–498.CrossRefGoogle Scholar
Basiuk, V.A. & Sainz-Rojas, J. (2001). Catalysis of peptide formation by inorganic oxides: High efficiency of alumina under mild conditions on the earth-like planets. Adv. Space Res. 27, 225–230.CrossRefGoogle ScholarPubMed
Bernal, J.D. (1951). The Physical Basis of Life. pp. 34–35. Routledge and Kegan Paul, London.Google Scholar
Brack, A. (2007). From interstellar amino acids to prebiotic catalytic peptides. Chem. Biodiverse 4, 665–679.CrossRefGoogle ScholarPubMed
Bujdak, J., Faybikova, K., Eder, A., Yongyai, Y. & Rode, B.M. (1995). Peptide chain elongation: A possible role of montmorillonite in prebiotic synthesis of protein precursors. Origins Life Evol. Biosphere 25, 431–441.CrossRefGoogle ScholarPubMed
Bujdak, J. & Rode, B.M. (1999). The effect of clay structure on peptide bond formation catalysis. J. Mol. Catalysis A: Chem. 144, 129–136.CrossRefGoogle Scholar
Bujdak, J. & Rode, B.M. (2004). On the mechanisms of oligopeptide reactions in solution and clay dispersion. J. Peptide Sci. 10, 731–737.CrossRefGoogle ScholarPubMed
Bujdak, J., Remko, M. & Rode, B.M. (2006). Selective adsorption and reactivity of dipeptide stereoisomer in clay mineral suspension. J. Colloid Interface Sci. 294, 304–308.CrossRefGoogle ScholarPubMed
Cairns-Smith, G. & Hartman, H. (1986). Clay minerals and the origin of life, Cambridge University Press, U.K.Google Scholar
Ferris, J.P., Hill, A.R. Jr, Liu, R. & Orgel, L.E. (1996). Synthesis of long prebiotic oligomeres on mineral surfaces. Nature. 381, 59–61.CrossRefGoogle Scholar
Fox, S.W. & Harada, K. (1960). The thermal copolymerization of amino acid common to protein. J. Am. Chem. Soc. 82, 3745–3751.CrossRefGoogle Scholar
Fox, S.W. & Dose, K. (1977). Molecular Evolution and the Origin of Life, Marcel Dekker, Inc., New York.Google Scholar
Grim, R.E. (1968). Clay mineralogy. 2nd edn. Mc Graw Hill Book Co., New York.Google Scholar
Hais, I.M. & Macke, K. (1963). Paper chromatography, pp. 110–169. Academic Press, New York.Google Scholar
Haldane, J.B.S. (1933). Science and human life. Origin Life Rational. pp. 149. Annu. Harper Bros., New York, London.Google Scholar
Hancock, W.S. & Harding, D.K.R. (1982). CRC Handbook of HPLC for Separation of Amino Acids, Peptides, Proteins and Nucleic Acids, Vol 1. CRC Press, Boca Raton, FL.Google Scholar
Howker, J.R. & Oro, J. (1981). Cyanamide mediated synthesis of peptides containing histidine and hydrophobic amino acids. J. Mol. Evol. 17, 285–294.CrossRefGoogle Scholar
Imai, E-I., Honda, H., Hatori, K., Brack, A. & Matsuno, K. (1999). Elongation of oligopepetides in a simulated submarine hydrothermal system. Science 283, 831–833.CrossRefGoogle Scholar
Kalra, S., Pant, C.K., Pathak, H.D. & Mehata, M.S. (2000). Adsorption of glycine and alanine on montmorillonite with or without coordinated divalent cations. Ind. J. Biochem. Biophys. 37, 341–346.Google Scholar
Kalra, S., Pant, C.K., Pathak, H.D. & Mehata, M.S. (2003). Studies on the adsorption of peptides of glycine/alanine on montmorillonite clay with or without coordinated divalent cations. Colloids Surf. A: Physicochem. Eng. Asp. 212, 43–50.CrossRefGoogle Scholar
Lahav, N. & Chang, S. (1976). The possible role of solid surface area in condensation reactions during chemical evolution. J. Mol. Evol. 8, 357–380.CrossRefGoogle ScholarPubMed
Lahav, N., White, D. & Chang, S. (1978). Peptide formation in prebiotic era, Thermal condensation of glycine in fluctuating clay environment. Science 201, 67–69.CrossRefGoogle Scholar
Lambert, J.F. (2008). Adsorption and polymerization of amino acids on mineral surfaces: a review. Origins Life Evol. Biosphere 38, 211–242.CrossRefGoogle ScholarPubMed
Lawless, J.G. & Levi, N. (1979). The role of metal ions in chemical evolution: polymerization of alanine and glycine in a cation exchanged clay environment. J. Mol. Evol. 13, 281.CrossRefGoogle Scholar
Lemmon, R.M. (1970). Chemical evolution. Chem. Rev. 70, 95–109.CrossRefGoogle Scholar
Matrajt, G. & Blanot, D. (2004). Properties of synthetic ferrihydrite as an amino acid adsorbent and a promoter of peptide bond formation. Amino Acids 26, 153–158.CrossRefGoogle Scholar
Meng, M., Stevano, L. & Lambert, J.F. (2004). Adsorption and thermal condensation mechanism of amino acids on oxide supports. 1. glycine on silica. Langmuir 20, 914–923.CrossRefGoogle Scholar
Oparin, A.I. (1924). Proiskhozhdenie zhizni, Moscovsky Robotshi Moscow (1957). The Origin of Life on Earth, 3rd edn, Academic Press, New York.Google Scholar
Paecht-Horowitz, M. & Eirich, F.R. (1988). The polymerization of amino acid adenylates on sodium-montmorillonite with pre adsorbed polypeptides. Origins Life Evol. Biosphere 18, 359–387.CrossRefGoogle Scholar
de Paiva, L.B., Morales, A.R. & Diaz, F.R.V. (2008). Organoclays: Properties, preparation and applications. Appl. Clay Sci. 42, 8–24.CrossRefGoogle Scholar
Plankenseiner, K., Righi, A. & Rode, B.M. (2002). Glycine and diglycine as possible catalytic factors in the prebiotic evolution of peptides. Origins Life Evol. Biosphere 32, 225–236.CrossRefGoogle Scholar
Ponnamperuma, C., Shimoyama, A. & Friebele, E. (1982). Clay and the origin of life. Origins Life 12, 9–40.CrossRefGoogle ScholarPubMed
Rao, M., Odom, D.G. & Oro, J. (1980). Clays in prebiotic chemistry. J. Mol. Evol. 15, 317–331.CrossRefGoogle Scholar
Rishpon, J., O'Hara, P.J., Lahav, N. & Lawless, J.G. (1982). Interaction between ATP, metal ions, glycine and several minerals. J. Mol. Evol. 18, 179–184.CrossRefGoogle ScholarPubMed
Rode, B.M. (1999). Peptides and the origin of life. Peptides 20, 773–786.CrossRefGoogle ScholarPubMed
Rode, B.M., Son, H.L., Suwanachot, Y. & Bujdak, J. (1999). The combination of salt induced peptide formation reaction and clay catalysis: A way to higher peptides under primitive earth conditions. Origins Life Evol. Biosphere 29, 273–286.CrossRefGoogle ScholarPubMed
Rohlfing, D.L. & McAlhaney, W.W. (1976). The thermal polymerization of amino acids in the presence of sand. Biosystems 8, 139–145.CrossRefGoogle ScholarPubMed
Schwendinger, M.G. & Rode, B.M. (1991). Salt-induced formation of mixed peptides under possible prebiotic conditions. Inorg. Chem. Acta 186, 247–251.CrossRefGoogle Scholar
Suwannachot, Y. & Rode, B.M. (1999). Mutual amino acid catalysis in salt induced peptide formation supports this mechanism's role in prebiotic peptide evolution. Origins Life Evol. Biosphere 29, 463–471.CrossRefGoogle ScholarPubMed
Theng, B.K.G. (1974). The Chemistry of Clay Organic Reactions, pp. 158–186. Wiley, New York.Google Scholar
Whitehouse, C. et al. (2005). Adsorption and self assembly of peptides on mica substrates. Angew. Chem. Int. Ed. 44, 1965–1968.CrossRefGoogle ScholarPubMed
Yanagawa, H., Kojima, K. & Ito, M.K. (1985). Thermophilic microspheres of peptide-like polymers and silicates at 250°C. J. Biochem. 97, 1521–1524.CrossRefGoogle Scholar
Zaia, D.A.M. (2004). A review of adsorption of amino acids on minerals: Was it important for origin of life? Amino Acids 27, 113–118.CrossRefGoogle ScholarPubMed