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Presence of Polyclonal Pseudomonas aeruginosa in an Intensive Care Unit A 27-Month Prospective Study on Molecular Epidemiology

Published online by Cambridge University Press:  02 January 2015

Pilar Cortés ,
Dolors Mariscal ,
Jordi Vallés ,
Jordi Rello  and
Pere Coll
Pilar Cortés
Affiliation:
Department of Microbiology, Hospital de la Santa Creu i Sant Pau, Universitat Autbnoma de Barcelona, Hospital de Sabadell, Corporacio Sanitaria Pare Tauli, Sabadell
Dolors Mariscal
Affiliation:
Microbiology Laboratory, Hospital de Sabadell, Corporacio Sanitaria Pare Tauli, Sabadell
Jordi Vallés
Affiliation:
Critical Care Department, Hospital de Sabadell, Corporacio Sanitaria Pare Tauli, Sabadell
Jordi Rello
Affiliation:
Critical Care Department, Hospital Universitari Joan XXIII, Universitat Rovira i Virgili, Tarragona, Spain
Pere Coll*
Affiliation:
Department of Microbiology, Hospital de la Santa Creu i Sant Pau, Universitat Autbnoma de Barcelona, Hospital de Sabadell, Corporacio Sanitaria Pare Tauli, Sabadell
*
Department of Microbiology, Hospital de la Santa Creu i Sant Pau, Avda Sant Antoni M Claret, 167, E-08025 Barcelona, Spain
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Abstract

Pseudomonas aeruginosa was isolated in 22.2% of 305 intensive care unit environmental cultures. A high genetic heterogeneity (18 pulsotypes) was evident. Taps and related surfaces were a stable reservoir for certain pulsotypes. The 15.4% of the P aerugi-Mosa-positive cultures were polyclonal. Different colony morpho-types should be assayed in surveillance studies.

Type
Concise Communications
Information
Infection Control & Hospital Epidemiology , Volume 22 , Issue 11 , November 2001 , pp. 720 - 723
Copyright
Copyright © The Society for Healthcare Epidemiology of America 2001

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References

1.Pollack, M. Pseudomonas aeruginosa. In: Mandell, GL, Bennett, JE, Dolin, R, eds. Principles and Practice of Infectious Diseases. 5th ed. Sidup, Kent, UK: Churchill Livingstone; 2000:2310–2335.Google Scholar
2.Talon, D, Cailleaux, V, Thouverez, M, Michel-Briand, Y. Discriminatory power and usefulness of pulsed-field gel electrophoresis in epidemiological studies of Pseudomonas aeruginosa. J Hosp Infect 1996;32:135–145.Google Scholar
3.Gilchrist, MJR. Epidemiologic and infection control microbiology. In: Isenberg, HD, ed. Clinical Microbiology Procedures Handbook. Washington, DC: American Society for Microbiology; 1992:vol 2:section 11.Google Scholar
4.Smith, CL, Hco, SR, Cantor, CR. Pulsed-field gel electrophoresis and the technology of large DNA molecules. In: Davies, KE, ed. Genome Analysis: A Practical Approach. Oxford, UK: IRL Press; 1988:41–72.Google Scholar
5.Tenover, FC, Arbeit, RD, Goering, RV, Mickelsen, PA, Murray, BE, Persing, DH, et al.Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: criteria for bacterial strain typing. J Clin Microbiol 1995;33:2233–2239.Google Scholar
6.Grundmann, H, Kropec, A, Hartung, D, Berner, R, Daschner, F. Pseudomonas aeruginosa in a neonatal intensive care unit: reservoirs and ecology of the nosocomial pathogen. J Infect Dis 1993;168:943–947.Google Scholar
7.Bophammer, J, Fiedler, B, Gudowius, P, von der Hardt, H, Romling, U, Tummler, B. Comparative hygienic surveillance of contamination with pseudomonads in a cystic fibrosis ward over a 4-year period. J Hosp Infect 1995;31:261–274.Google Scholar
8.Widmer, AF, Wenzel, RP, Trilla, A, Bale, MJ, Jones, RN, Doebbeling, BN. Outbreak of Pseudomonas aeruginosa infections in a surgical intensive care unit: probable transmission via hands of a health care worker. Clin Infect Dis 1993;16:372–376.Google Scholar
9.Bonten, MJM, Bergmans, DCJJ, Speijer, H, Stobberingh, EE. Characteristics of polyclonal endemicity of Pseudomonas aeruginosa colonization in intensive care units. Am J Respir Crit Care Med 1999;160:1211–1219.Google Scholar
10.Goering, RV, Tenover, FC. Epidemiological interpretation of chromosomal macro-restriction fragment patterns analyzed by pulsed-field gel electrophoresis. J Clin Microbiol 1997;35:2432–2433.Google Scholar