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In Vitro Assessment of Chlorhexidine Gluconate–Impregnated Polyurethane Foam Antimicrobial Dressing Using Zone of Inhibition Assays

Published online by Cambridge University Press:  02 January 2015

Shubhangi Bhende*
Affiliation:
Corporate Microbiology and Sterilization Sciences, Ethicon, Inc., Somerville, New Jersey
Daniel Spangler
Affiliation:
Corporate Microbiology and Sterilization Sciences, Ethicon, Inc., Somerville, New Jersey
*
Ethicon, Inc., P.O. Box 151, Somerville, NJ 08876

Abstract

Objective:

To evaluate an antimicrobial dressing consisting of hydrophilic polyurethane foam with chlorhexidine gluconate for activity against several antibiotic-resistant clinical isolates as well as American Type Culture Collection reference strains using zone of inhibition assays.

Methods:

Sterile foam samples with chlorhexidine gluconate and untreated controls were transferred onto inoculated agar plates. Plates were incubated at 35°C to 37° C for 24 hours and examined for zones of inhibition around the foam samples.

Results:

Polyurethane foam with chlorhexidine gluconate showed antimicrobial activity in vitro against all of the challenge organisms including antibiotic-resistant clinical isolates.

Conclusion:

The data from this in vitro study support the hypothesis that polyurethane foam with chlorhexidine gluconate has an antimicrobial effect against antibiotic-resistant Staphylococcus and Enterococcus species, as well as Candida species.

Type
Original Articles
Copyright
Copyright © The Society for Healthcare Epidemiology of America 2004

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References

1.Malchesky, P, Chamberlain, V, Scott-Conner, C, Salis, B, Wallace, C. Reprocessing of reusable medical devices. ASAIO J 1995;41:146151.Google Scholar
2.Mermel, LA. Prevention of intravascular catheter-related infections. Ann Intern Med 2000;132:391402.Google Scholar
3.Rello, J, Ochagavia, A, Sabanes, E, et al.Evaluation of outcome of intravenous catheter-related infections in critically ill patients. Am J Respir Crit Care Med 2000;162:10271030.Google Scholar
4.Dimick, JB, Pelz, RK, Consunji, R, et al.Increased resource use associated with catheter-related bloodstream infection in a surgical intensive care unit. Arch Surg 2001;136:229234.Google Scholar
5.Mermel, LA. Catheter-related bloodstream infections [correction]. Ann Intern Med 2000;133:395.Google Scholar
6.Steckelberg, JM, Osmon, DR. Prosthetic joint infections. In: Bisno, AL, Waldvogel, FA, eds. Infections Associated With Indwelling Medical Devices, ed. 3. Washington, DC: ASM Press; 2000:173209.Google Scholar
7.Campbell, AA, Song, L, Nelson, BJ, et al.Development, characterization, and anti-microbial efficacy of hydroxyapatite-chlorhexidine coatings produced by surface-induced mineralization. J Biomed Mater Res 2000;53:400407.Google Scholar
8.Moroni, A, Vannini, F, Mosca, M, Giannini, S. State of the art review: techniques to avoid pin loosening and infection in external fixation. J Orthop Trauma 2002;16:189195.CrossRefGoogle ScholarPubMed
9.Lewallen, DG, Edwards, CC. Complications of external fixation. In: Epps, CH Jr ed. Complications of Orthopedic Surgery, ed. 3. Philadelphia: J. B. Lippincott; 1994.Google Scholar
10.Pearson, ML. Guideline for prevention of intravascular device-related infections. Infect Control Hosp Epidemiol 1996;17:438473.Google Scholar
11.Sherertz, RJ. Pathogenesis of vascular catheter infection. In: Bisno, AL, Waldvogel, FA, eds. Infections Associated With Indwelling Medical Devices, ed. 3. Washington, DC: ASM Press; 2000:111125.Google Scholar
12.Jarvis, WR, Edwards, JR, Culver, DH, et al.Nosocomial infection rates in adult and pediatric intensive care units in the United States. Am J Med 1991;91(suppl 3B):185S191S.Google Scholar
13.Toltzis, P, Goldmann, DA. Current issues in central venous catheter infection. Ann Rev Med 1990;41:169176.Google Scholar
14.Maki, DG. Infections caused by intravascular devices used for infusion therapy: pathogenesis, prevention and management. In: Bisno, AL, Waldvogel, FA, eds. Infections Associated With Indwelling Medical Devices, ed. 2. Washington, DC: ASM Press; 1994:155212.Google Scholar
15.Sherertz, PJ. Pathogenesis of vascular catheter-related infections. In: Seifert, H, Jansen, B, Farr, BM, eds. Catheter-Related Infections. New York: Marcel Dekker; 1997:130.Google Scholar
16.Centers for Disease Control and Prevention. National Nosocomial Infections Surveillance (NNIS) System report: data summary from January 1990-May 1999, issued June 1999. Am J Infect Control 1999;27:520532.Google Scholar
17.Maki, DG, Narans, LL, Kluger, DM. Prospective randomized, investigator-masked trial of a novel chlorhexidine-impregnated disk (BIOPATCH) on central venous and arterial catheters. Infect Control Hosp Epidemiol 2000;21:96.Google Scholar
18.Maki, DG. Preventing intravascular catheter-related infections: an evidence-based approach. Physician Weekly 2004;21. Available at www.physweekly.com/pc.asp?issuid=66&questionid=72. Accessed December 2003.Google Scholar
19.Banton, J, Banning, V. Impact of catheter-related bloodstream infections with the use of the BIOPATCH dressing. Journal of Vascular Access Devices 2002:2732.Google Scholar
20.Crnich, CJ, Maki, DG. The promise of novel technology for the prevention of intravascular device-related bloodstream infection: 1. Pathogenesis and short-term devices. Clin Infect Dis 2002;34:12321242.Google Scholar
21.Bootman, M, Yamamoto, R, inventors; Integra LifeSciences I Ltd., assignee. Method of making a polyurethane-biopolymer composite. US patent 6,071,447. June 6, 2000.Google Scholar
22.Russell, AD. Principles of antimicrobial activity and resistance. In: Block, S, ed. Disinfection, Sterilization, and Preservation, ed. 5. Philadelphia: Lippincott Williams & Wilkins; 2001:4749.Google Scholar
23.Russell, AD. Bacterial sensitivity and resistance, plasmids and bacterial resistance. In: Russell, AD, Hugo, WB, Ayliffe, GAJ, eds. Principles and Practice of Disinfection, Preservation, and Sterilization, ed. 3. Oxford, England: Blackwell Science; 1999:299301.Google Scholar
24.Stickler, DJ, Thomas, B. Antiseptic and antibiotic resistance in gram-negative bacteria causing urinary tract infection. J Clin Pathol 1980;33:288296.Google Scholar