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Contamination of Trypan Blue With Burkholderia cepacia in a Cornea Bank

Published online by Cambridge University Press:  02 January 2015

Pascal C. Morel ,
Nora Roubi ,
Daniel R. Talon  and
Xavier Bertrand
Pascal C. Morel
Affiliation:
Unité de Thérapie Cellulaire et Génique, Etablissement Français du Sang, Besançon, France
Nora Roubi
Affiliation:
Unité de Thérapie Cellulaire et Génique, Etablissement Français du Sang, Besançon, France
Daniel R. Talon
Affiliation:
Service d'Hygiène hospitalière et d’Epidémiologie moléculaire, Centre Hospitalier Universitaire Jean Minjoz, Besançon, France
Xavier Bertrand*
Affiliation:
Service d'Hygiène hospitalière et d’Epidémiologie moléculaire, Centre Hospitalier Universitaire Jean Minjoz, Besançon, France
*
Service d’Hygiène hospitalière et d'Epidémiologie moléculaire, Centre Hospitalier Universitaire Jean Minjoz, 25030 Besançon, France
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Abstract

Objective:

To describe Burkholderia cepacia contamination of a cornea bank and the measures taken to identify and eliminate the source of infection.

Methods:

Cultures were performed to assess the extent and source of contamination, and pulsed-fleld gel electrophoresis was used for molecular typing.

Results:

Routine surveillance cultures identified 5 contaminated corneas during a 10-day period. Additional cultures showed that 28 of 88 samples were positive for this organism. Environmental investigation showed that an open bottle of trypan blue used to assess corneal morphology was contaminated with the epidemic strain.

Conclusion:

Trypan blue played a major role in this contamination of corneas. This episode shows that microbial contamination can affect transplanted corneas despite ongoing culture surveillance and suggests that new methods may be needed to avoid this risk.

Type
Orginal Articles
Information
Infection Control & Hospital Epidemiology , Volume 24 , Issue 3 , March 2003 , pp. 198 - 201
Copyright
Copyright © The Society for Healthcare Epidemiology of America 2003

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