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Clinical and Molecular Characteristics of an Outbreak Caused by the Pandemic (BI/NAP1/027) Clostridium difficile Clone in a Single Center in Israel

Published online by Cambridge University Press:  10 May 2016

Yonit Wiener-Well*
Affiliation:
Infectious Disease Unit, Shaare Zedek Medical Center, affiliated with Hadassah-Hebrew University Medical School, Jerusalem, Israel
Eli Ben-Chetrit
Affiliation:
Infectious Disease Unit, Shaare Zedek Medical Center, affiliated with Hadassah-Hebrew University Medical School, Jerusalem, Israel
Mustafa Abed-Eldaim
Affiliation:
Internal Medicine, Shaare Zedek Medical Center, affiliated with Hadassah-Hebrew University Medical School, Jerusalem, Israel
Marc V. Assous
Affiliation:
Clinical Microbiology Laboratory, Shaare Zedek Medical Center, affiliated with Hadassah-Hebrew University Medical School, Jerusalem, Israel
Tamar Miller-Roll
Affiliation:
National Center of Infection Control, Ministry of Health, affiliated with Tel-Aviv University Sackler Faculty of Medicine, Tel-Aviv, Israel
Amos Adler
Affiliation:
National Center of Infection Control, Ministry of Health, affiliated with Tel-Aviv University Sackler Faculty of Medicine, Tel-Aviv, Israel
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Abstract

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Type
Research Brief
Copyright
© 2014 by The Society for Healthcare Epidemiology of America. All rights reserved.

References

1. Magill, SS, Edwards, JR, Bamberg, W, et al. Multistate point-prevalence survey of health care–associated infections. N Engl J Med 2014;370:11981208.CrossRefGoogle ScholarPubMed
2. Warny, M, Pepin, J, Fang, A, et al. Toxin production by an emerging strain of Clostridium difficile associated with outbreaks of severe disease in North America and Europe. Lancet 2005;366:10791084.CrossRefGoogle Scholar
3. McDonald, LC, Killgore, GE, Thompson, A, et al. An epidemic, toxin gene–variant strain of Clostridium difficile . N Engl J Med 2005;353:24332441.CrossRefGoogle ScholarPubMed
4. Clements, ACA, Magalhães, RJS, Tatem, AJ, Paterson, DL, Riley, TV. Clostridium difficile PCR ribotype 027: assessing the risks of further worldwide spread. Lancet Infect Dis 2010;10:395304.Google Scholar
5. Alzahrani, N, Johani, SA. Emergence of a highly resistant Clostridium difficile strain (NAP/BI/027) in a tertiary care center in Saudi Arabia. Ann Saudi Med 2013;33:198199.CrossRefGoogle Scholar
6. Bishara, J, Goldberg, E, Madar-Shapiro, L, Behor, J, Samra, Z. Molecular epidemiology of Clostridium difficile in a tertiary medical center in Israel: emergence of the polymerase chain reaction ribotype 027. Isr Med Assoc J 2011;13:338341.Google Scholar
7. Clinical and Laboratory Standards Institute (CLSI). Methods for Antimicrobial Susceptibility Testing of Anaerobic Bacteria: Approved Standard—Eighth Edition. Wayne, PA: CLSI, 2012.Google Scholar
8. Lemee, L, Dhalluin, A, Testelin, S, et al. Multiplex PCR targeting tpi (triose phosphate isomerase), tcdA (toxin A), and tcdB (toxin B) genes for toxigenic culture of Clostridium difficile . J Clin Microbiol 2004;42:57105714.CrossRefGoogle ScholarPubMed
9. Persson, S, Jensen, JN, Olsen, KEP. Multiplex PCR method for detection of Clostridium difficile tcdA, tcdB, cdtA, and cdtB and internal in-frame deletion of tcdC. J Clin Microbiol 2011;49:42994300.Google Scholar
10. Kato, H. Typing by sequencing the slpA gene of Clostridium difficile strains causing multiple outbreaks in Japan. J Med Microbiol 2005;54:167171.CrossRefGoogle ScholarPubMed