Hostname: page-component-546b4f848f-bvkm5 Total loading time: 0 Render date: 2023-06-04T04:02:37.367Z Has data issue: false Feature Flags: { "useRatesEcommerce": true } hasContentIssue false

Preventing Antibiotic Resistance Using Rapid DNA-Based Diagnostic Tests

Published online by Cambridge University Press:  02 January 2015

Michel G. Bergeron*
Centre de Recherche en Infectiologie du Centre de Recherche du CHUL et Département de Microbiologie, Université Laval, Québec, Canada
Marc Ouellette
Centre de Recherche en Infectiologie du Centre de Recherche du CHUL et Département de Microbiologie, Université Laval, Québec, Canada
Centre Hospitalier de l'Université Laval, 2705 boul Laurier, Ste-Foy, Québec, Canada G1V 4G2; e-mail,


Improved diagnostic procedures should be an effective way to control infectious diseases and the spread of antibiotic resistance. To do so, diagnostics will need to be obtained within 1 hour of sampling and will require nucleic acid-based amplification tests directly on the clinical specimen.

From the Fifth International Conference on the Prevention of Infection
Copyright © The Society for Healthcare Epidemiology of America 1998

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)


1.Crow, S. Asepsis: back to the basics [In Process Citation]. Urol Nurs 1998;18:4246.Google Scholar
2.Gold, HS, Moellering, RC Jr. Antimicrobial-drug resistance. N Engl J Med 1996;335:14451453.Google Scholar
3.Tomasz, A. Multiple-antibiotic-resistant pathogenic bacteria. A report on the Rockefeller University Workshop. N Engl J Med 1994;330:12471251.Google Scholar
4.Booy, R, Kroll, JS. Bacterial meningitis and meningococcal infection. Curr Opin Pediatr 1998;10:1318.Google Scholar
5.Cohen, HA, Matalon, A, Amir, J, Paret, G, Barzilai, A. Handwashing patterns in primary pediatric community clinics. Infection 1998;26:4547.Google Scholar
6.Scheckler, WE, Brimhall, D, Buck, AS, Farr, BM, Friedman, C, Garibaldi, RA, et al.Requirements for infrastructure and essential activities of infection control and epidemiology in hospitals: a consensus panel report. Infect Control Hosp Epidemiol 1998;19:114124.Google Scholar
7.Voss, A, Widmer, AF. No time for handwashing!? Handwashing versus alcoholic rub: can we afford 100% compliance? Infect Control Hosp Epi demiol 1997;18:205208.Google Scholar
8.Ouellette, M, Kündig, C. Microbial multidrug resistance. Int J Antimicrob Agents 1997;8:179187.Google Scholar
9.Bergeron, MG, Ouellette, M. Diagnosing bacterial infectious diseases in one hour: an essential upcoming revolution. Infection 1995;23:6972.Google Scholar
10.Bergeron, MG, Ouellette, M. Preventing antibiotic resistance through rapid genotypic identification of bacteria and of their antibiotic resistance genes in the clinical microbiology laboratory. J Clin Microbiol In press.Google Scholar
11.Vogel, F, Luth, A, Charpentier, A. The impact of microbiological diagnostics on the antimicrobial treatment of hospitalised patients with infectious disease. Eur J Med Res 1996;1:312314.Google Scholar
12.McCraig, LF, Hughes, JM. Trends in antimicrobial drug prescribing among office-based physicians in the United States. JAMA 1995;273: 214219.Google Scholar
13.Tenover, FC, Unger, ER. Nucleic Acid Probes for Detection and Identification of Infectious Agents. Persing, DH, Smith, TF, Tenover, FC, White, TJ, eds. Washington, DC: American Society for Microbiology. 1993.Google Scholar
14.Wolcott, MJ. Advances in nucleic acid-based detection methods. Clin Microbiol Rev 1992;5:370386.Google Scholar
15.Martineau, F, Picard, FJ, Roy, PH, Ouellette, M, Bergeron, MG. Species- specific and ubiquitous DNA-based assays for rapid identification of Staphylococcus aureus [In Process Citation]. J Clin Microbiol 1998;36:618623.Google Scholar
16.Olsen, GJ, Woese, CR. Ribosomal RNA: a key to phylogeny. Faseb J 1993;7:113123.Google Scholar
17.Greisen, K, Loeffelholz, M, Purohit, A, Leong, D. PCR primers and probes for the 16S rRNA gene of most species of pathogenic bacteria, including bacteria found in cerebrospinal fluid. J Clin Microbiol 1994;32:335351.Google Scholar
18.Hall, LM, Duke, B, Urwin, G. An approach to the identification of the pathogens of bacterial meningitis by the polymerase chain reaction. Eur J Clin Microbiol Infect Dis 1995;14:10901094.Google Scholar
19.Jalava, J, Mantymaa, ML, Ekblad, U, Toivanen, P, Skurnik, M, Lassila, O, et al.Bacterial 16S rDNA polymerase chain reaction in the detection of intra-amniotic infection. Br J Obstet Gynaecol 1996;103:664669.Google Scholar
20.Heritz, DM, Lacroix, JM, Batra, SD, Jarvi, KA, Beheshti, B, Mittelman, MW. Detection of eubacteria in interstitial cystitis by 16S rDNA amplification. J Urol 1997;158:22912295.Google Scholar
21.Arlet, G, Philippon, A. PCR-Based Approaches for the Detection of Bacterial Resistance. Ehrlich, GD, Greenberg, SJ, eds. Blackwell Scientific Publications; 1994.Google Scholar
22.Courvalin, P. Genotypic approach to the study of bacterial resistance to antibiotics. Antimicrob Agents Chemother 1991;3:10191023.Google Scholar
23.Persing, DH, Relman, DA, Tenover, FC. Genotypic Detection of Antimicrobial Resistance. Persing, D, ed. Washington, DC: ASM Press; 1996.Google Scholar
24.Tenover, FC. Bauer and Kirbey meet Watson and Crick: antimicrobial testing in the molecular era. ASM News 1992;58;669672.Google Scholar
25.Jacoby, GA. Extended-spectrum beta-lactamases and other enzymes providing resistance to oxyimino-beta-lactams. Infect Dis Clin North Am 1997;11:875887.Google Scholar
26.Arthur, M, Molinas, C, Mabilat, C, Courvalin, P. Detection of erythromycin resistance by the polymerase chain reaction using primers in conserved regions of erm rRNA methylase genes. Antimicrob Agents Chemother 1990;34:20242026.Google Scholar
27.Coffey, TJ, Daniels, M, McDougal, LK, Dowson, CG, Tenover, FC, Spratt, BG. Genetic analysis of clinical isolates of Streptococcus pneumoniae with high-level resistance to expanded spectrum cephalosporins. Antimicrob Agents Chemother 1995;3:13061313.Google Scholar
28.Acar, JF, Goldstein, FW. Trends in bacterial resistance to fluoroquinolones. Clin Infect Dis 1997;24(suppl 1):S67S73.Google Scholar
29.Fodor, SP, Rava, RP, Huang, XC, Pease, AC, Holmes, CP, Adams, CL. Multiplexed biochemical assays with biological chips. Nature 1993;364:555556.Google Scholar
30.Lipshutz, RJ, Morris, D, Chee, M, Hubbell, E, Kozal, MJ, Shah, N, et al.Using oligonucleotide probe arrays to access genetic diversity. Biotechniques 1995;19:4427.Google Scholar
31.Chambers, H. Methicillin resistance in staphylococci: molecular and biochemical basis and clinical implications. Clin Microbiol Rev 1997;10: 781791.Google Scholar
32.Michel, M, Gutmann, L. Methicillin-resistant Staphylococcus aureus and vancomycin-resistant enterococci: therapeutic realities and possibilities. Lancet 1997;349:19011906.Google Scholar
33.Carroll, KC, Leonard, RB, Newcomb-Gayman, PL, Hillyard, DR. Rapid detection of the staphylococcal mecA gene from BACTEC blood culture bottles by the polymerase chain reaction. Am J Clin Pathol 1996;106: 600605.Google Scholar
34.Wallet, F, Roussel-Delvallez, M, Courcol, RJ. Choice of a routine method for detecting methicillin resistance in staphylococci. J Antimicrob Chemother 1996;37:901909.Google Scholar
35.Arthur, M, Reynolds, P, Courvalin, P. Glycopeptide resistance in enterococci. Trends Microbiol 1996;4:401407.Google Scholar
36.Poyart, C, Pierre, C, Quesne, G, Pron, B, Berche, P, Trieu-Cuot, P. Emergence of vancomycin resistance in the genus Streptococcus: characterization of a vanB transferable determinant in Streptococcus bovis. Antimi-crob Agents Chemother 1997;41:2429.Google Scholar
37.Dutka-Malen, S, Evers, S, Courvalin, P. Detection of glycopeptide resistance genotypes and identification to the species level of clinically relevant enterococci by PCR. J Clin Microbiol 1995;33:1434.Google Scholar
38.Patel, R, Uhl, JR, Kohner, P, Hopkins, MK, Cockerill, FR III. Multiplex PCR detection of vanA, vanB, vanC-1, and vanC-2/3 genes in enterococci. J Clin Microbiol 1997;35:703707.Google Scholar
39.Perichon, B, Reynolds, P, Courvalin, P. VanD-type glycopeptide-resistant Enterococcus faecium BM4339. Antimicrob Agents Chemother 1997;41:20162018.Google Scholar