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Repression of induction by u.v. of λ phage by exrA mutations in Escherichia coli

Published online by Cambridge University Press:  14 April 2009

John Donch ,
Joseph Greenberg  and
Michael H. L. Green
John Donch
Affiliation:
Palo Alto Medical Research Foundation, 860 Bryant Street, Palo Alto, California 94301
Joseph Greenberg
Affiliation:
Palo Alto Medical Research Foundation, 860 Bryant Street, Palo Alto, California 94301
Michael H. L. Green
Affiliation:
Palo Alto Medical Research Foundation, 860 Bryant Street, Palo Alto, California 94301
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The effect of ultraviolet radiation (u.v.) on λ lysogens of exrA strains of Escherichia coli was studied. exrA strains could be lysogenized with, as well as support the vegetative reproduction of, λ. However, though spontaneous induction of λ occurred in exrA(λ) strain at 10% the frequency of exrA+(λ) strain, exrA(λ) strains were not induced by u.v. Because λ was not induced in exrA(λ) strains, lysogens of these strains were no more sensitive to u.v. than were non-lysogens.

The heat-inducible mutant λhcI857 could be induced in exrA strains at elevated temperatures. Furthermore, u.v. irradiation of exrA (λhcI857) strain did not prevent the heat induction of this λ mutant. The exrA mutation appeared to interfere only with the inactivation of λ repressor by u.v.

Among the exrA strains studied was strain Bs1(exr A uvrB). Whereas the λ lysogen of strain Bsl could not be induced by u.v. and was no more sensitive to u.v. than its non-lysogen, the exrA+uvrB(λ) derivative of strain Bsl could be induced by u.v. and was more sensitive to u.v. than its non-lysogen.

Type
Research Article
Information
Genetics Research , Volume 15 , Issue 1 , February 1970 , pp. 87 - 97
Copyright
Copyright © Cambridge University Press 1970

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