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Mutants of Coprinus lagopus selected for resistance to 2-deoxy-D-glucose

Published online by Cambridge University Press:  14 April 2009

David Moore  and
George R. Stewart
David Moore
Affiliation:
The Department of Botany, The University, Manchester, M13 9PL
George R. Stewart
Affiliation:
The Department of Botany, The University, Manchester, M13 9PL
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The glucose analogue 2-deoxy-D-glucose seriously inhibits the growth of Coprimts lagopus. Following treatment with N-methyl-N′-nitro-N-nitrosoguanidine 388 resistant mutants were isolated. It is shown that the mutants isolated are probably allelic; they were phenotypically similar and no complementation was observed. The mutants were pleiotropic in the sense that although they were initially selected only for resistance to 2-deoxy-D-glucose they were found to be cross-resistant to both of the related analogues, sorbose and glucosamine. Furthermore, the mutants were unable to utilize fructose as a sole carbon source. It is demonstrated that the inability to utilize fructose results from a defect in sugar transport. The gene symbol ftr is proposed for this cistron and it is shown that though the gene is quite closely linked to its own centromere, it is unlinked to centromere markers of the six known linkage groups.

Type
Research Article
Information
Genetics Research , Volume 18 , Issue 3 , December 1971 , pp. 341 - 352
Copyright
Copyright © Cambridge University Press 1971

References

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