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Mutant with heat-sensitive capacity for phagocytosis in tetrahymena: isolation and genetic characterization

Published online by Cambridge University Press:  14 April 2009

Gary B. Silberstein ,
Eduardo Orias  and
Nina A. Pollock
Gary B. Silberstein
Affiliation:
Section of Biochemistry and Molecular Biology, Department of Biological Sciences, University of California at Santa Barbara, Santa Barbara, California 93106, U.S.A.
Eduardo Orias
Affiliation:
Section of Biochemistry and Molecular Biology, Department of Biological Sciences, University of California at Santa Barbara, Santa Barbara, California 93106, U.S.A.
Nina A. Pollock
Affiliation:
Section of Biochemistry and Molecular Biology, Department of Biological Sciences, University of California at Santa Barbara, Santa Barbara, California 93106, U.S.A.

Summary

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A mutant of Tetrahymena with heat-sensitive phagocytosis was obtained using a tantalum-particle enrichment procedure. The mutant phenotype is most likely determined by a somatic (macronuclear) mutation(s). The inability of the mutant to sustain cell division and to phagocytize at 37 °C are most likely determined by the same mutation. The phenotype of the mutant is stably inherited under vegetative propagation at 30 °C. At 37 °C, the mutation affects the development of the oral apparatus, the phagocytotic organelle. This mutant has proven useful for the study of cellular functions related to phagocytosis.

Type
Research Article
Information
Genetics Research , Volume 26 , Issue 1 , August 1975 , pp. 11 - 19
Copyright
Copyright © Cambridge University Press 1975

References

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