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Mutagenesis in Escherichia coli

III. Requirement for DNA synthesis in mutation by gamma rays of T4-phage complexed with Escherichia coli

Published online by Cambridge University Press:  14 April 2009

B. A. Bridges ,
Rachel E. Dennis  and
R. J. Munson
B. A. Bridges
Affiliation:
M.R.C. Radiology Unit, Harwell, Didcot, Berkshire, U.K.
Rachel E. Dennis
Affiliation:
M.R.C. Radiology Unit, Harwell, Didcot, Berkshire, U.K.
R. J. Munson
Affiliation:
M.R.C. Radiology Unit, Harwell, Didcot, Berkshire, U.K.
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A system has been developed for the study of reversion of an amber mutation responsible for a deficiency in DNA synthesis in T4 phage E51. When complexed with bacteria able to suppress the amber mutation the induced mutation rate per phage genome per rad is

When complexed with bacteria unable to suppress the amber mutation (and being thus unable to synthesize phage DNA) the induced mutation rate is at least 14 times lower indicating that DNA synthesis is necessary for the production of the majority of functional reversions at the amber site. The induced mutation rate in suppressor-containing bacteria is independent of multiplicity of infection between 0·2 and 5, suggesting that recombination immediately after irradiation between phage genomes is unlikely to be a requirement for the mutation process.

Type
Research Article
Information
Genetics Research , Volume 15 , Issue 2 , April 1970 , pp. 147 - 156
Copyright
Copyright © Cambridge University Press 1970

References

REFERENCES

Adams, M. H. (1959). Bacteriophages. New York: Interscience.CrossRefGoogle Scholar
Bridges, B. A. (1969). Mechanisms of radiation mutagenesis in cellular and subcellular systems. Ann. Rev. Nucl. Sci. 19 (in Press).CrossRefGoogle ScholarPubMed
Bridges, B. A., Dennis, R. E. & Munson, R. J. (1967). Differential induction and repair of ultraviolet damage leading to true reversions and external suppressor mutations of an ochre codon in Escherichia coli B/r WP2. Genetics 57, 897–908.CrossRefGoogle Scholar
Bridges, B. A., Law, J. & Munson, R. J. (1968). Mutagenesis in Escherichia coli. II. Evidence for a common pathway for mutagenesis by ultraviolet light, ionizing radiation and thymine deprivation. Molec. gen. Genet. 103, 266–273.CrossRefGoogle ScholarPubMed
Bridges, B. A. & Munson, R. J. (1968). Mutagenesis in Escherichia coli: evidence for the mechanism of base change mutation by ultraviolet radiation in a strain deficient in excision repair. Proc. Roy. Soc. Lond. B 171, 213–226.Google Scholar
Emmerson, P. T. & Howard-Flanders, P. (1965) Post-irradiation degradation of DNA following exposure of ultraviolet-sensitive and resistant bacteria to X-rays. Biochem. biophys. res. Commun. 18, 24–29.CrossRefGoogle Scholar
Grigg, G. W. (1964). Radiation-induced reversion of transition mutants. Austr. J. Biol. Sci. 17, 907–920.CrossRefGoogle Scholar
Kada, T., Doudney, C. O. & Haas, F. L. (1961). Some biochemical factors in X-ray induced mutation in bacteria. Genetics 46, 683–702.CrossRefGoogle ScholarPubMed
Lark, K. G., Repko, T. & Hoffman, E. J. (1963). The effect of amino acid deprivation on subsequent deoxyribonucleic acid replication. Biochem. biophys. Acta 76, 9–24.CrossRefGoogle ScholarPubMed
Maaløe, O. & Hanawalt, P. C. (1961). Thymine deficiency and the normal DNA replication cycle. I. J. molec. Biol. 3, 144–155.CrossRefGoogle ScholarPubMed
Magni, G. E. & Von Borstel, R. C. (1962). Different rates of spontaneous mutation during mitosis and meiosis in yeast. Genetics 47, 1097–1108.Google ScholarPubMed
Munson, R. J. & Bridges, B. A. (1964). Segregation of radiation-induced mutations in Escherichia coli. Nature, Lond. 203, 270–272.CrossRefGoogle ScholarPubMed
Munson, R. J. & Bridges, B. A. (1966). Site of lethal damage by ionizing radiation in Escherichia coli B/r growing exponentially in minimal medium. Nature, Lond. 210, 922–925.CrossRefGoogle ScholarPubMed
Munson, R. J. & Bridges, B. A. (1969). Lethal and mutagenic lesions induced by ionizing radiations in E. coli and DNA strand breaks. Biophysik 6, 1–6.Google Scholar
Nishioka, H. & Doudney, C. O. (1969). Different modes of loss of photoreversibility of mutation and lethal damage in ultraviolet light resistant and sensitive bacteria. Mutation Res. (in Press).CrossRefGoogle Scholar
Ponnamperuma, C. A., Lemmon, R. M. & Calvin, M. (1962). Chemical effect of ionizing radiation on cytosine. Science 137, 605–607.CrossRefGoogle ScholarPubMed
Stuy, J. H. (1960). Studies on the radiation inactivation of microorganisms. VI. X-ray induced breakdown of deoxyribonucleic acid in Haemophilus influenzae and in other bacteria. J. Bact. 79, 707–715.CrossRefGoogle ScholarPubMed
Wiberg, J. S. (1966). Mutants of bacteriophage T4 unable to cause breakdown of host DNA. Proc. natn. Acad. Sci. U.S.A. 55, 614–621.Google ScholarPubMed
Witkin, E. M. (1967). Radiation-induced mutations and their repair. Science 152, 1345–1353.CrossRefGoogle Scholar
Witkin, E. M. (1969). The role of DNA repair and recombination in mutagenesis. Proc. XII Int. Congr. Genetics (in Press).Google Scholar