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The epistasis of Adh and Gpdh allozymes and variation in the ethanol tolerance of Drosophila melanogaster larvae

Published online by Cambridge University Press:  14 April 2009

Stephen W. McKechnie  and
Billy W. Geer
Stephen W. McKechnie*
Affiliation:
Department of Genetics, Monash University, Clayton 3168, Australia
Billy W. Geer
Affiliation:
Department of Biology, Knox College, Galesburg, Illinois 61401, USA
*
* Corresponding author.

Summary

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The role of epistatic interaction of allozymes in the determination of variation in larval ethanol tolerance of Drosophila melanogaster was examined. Isofemale lines from the Tahbilk Winery were made homozygous for different common alleles of alcohol dehydrogenase (Adh) and sn-glycerol-3-phosphate dehydrogenase (Gpdh). When fed 6% ethanol, all the lines had reduced survival and, in the survivors, reduced body weight and lengthened development time. A strong positive correlation between tolerance and development time suggested that alleles responsible for slowing development on ethanol also increased ethanol tolerance. Analysis of larval ethanol tolerance over four generations showed that larvae of the AdhffGpdhff, and AdhssGpdhss allelic combinations were more tolerant than larvae with the other combinations. However, these genotypes were not associated with the slowing of development nor the weight loss on ethanol. Hence, larvae with certain combinations of Adh and Gpdh allozymes may have a greater capacity to metabolize ethanol and be more tolerant to its toxic effects.

Type
Research Article
Information
Genetics Research , Volume 52 , Issue 3 , December 1988 , pp. 179 - 184
Copyright
Copyright © Cambridge University Press 1988

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