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The characterization of an alanine racemase mutant of Escherichia coli

Published online by Cambridge University Press:  14 April 2009

H. J. W. Wijsman
Institute of Genetics, University of Amsterdam, Amsterdam, the Netherlands
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Among temperature-sensitive mutants of Escherichia coli a strain was discovered requiring D-alanine for growth. It was proved to possess an altered alanine racemase. The structural gene for this enzyme, designated alr, is located between metB and pur A. The properties of the enzyme and its locus suggest that it is not under control of the mechanisms which regulate mucopeptide formation. A suppressor of the alr mutation was discovered near trp, and termed msuA.

Research Article
Copyright © Cambridge University Press 1972



Berberich, R., Kaback, M. & Freese, E. (1968). Amino acids as inducers of L-alanine dehydrogenase in Bacillus subtilis. Journal of Biological Chemistry 243, 10061011.Google ScholarPubMed
Böck, A. & Neidhardt, F. C. (1966). Isolation of a mutant of Escherichia coli with a temperature-sensitive fructose-1,6-diphosphate aldolase activity. Journal of Bacteriology 92, 464469.Google ScholarPubMed
Broda, P. (1967). The formation of Hfr strains in Escherichia coli K 12. Genetical Research, Cambridge 9, 3547.CrossRefGoogle Scholar
Eidlic, L. & Neidhardt, F. C. (1965). Protein and nucleic acid synthesis in two mutants of Escherichia coli with temperature-sensitive aminoacyl ribonucleic acid synthetases. Journal of Bacteriology 89, 706711.Google ScholarPubMed
Lennox, E. S. (1955). Transduction of linked genetic characters of the host by bacteriophage P 1. Virology 1, 190206.CrossRefGoogle Scholar
Lugtenberg, E. J. J. & van Schijndel-van Dam, A. (1973). A temperature sensitive mutant of Escherichia coli K12 with an impaired D-alanine: D-alanine ligase. Journal of Bacteriology, (in the Press).Google Scholar
Mangiarotti, G., Apirion, D. & Schlessinger, D. (1966). Selection of sucrose dependent Escherichia coli to obtain envelope mutants and fragile cultures. Science, New York 153, 892894.CrossRefGoogle ScholarPubMed
Matsuzawa, H., Matsuhashi, M., Oka, A. & Sugino, Y. (1969). Genetic and biochemical studies on cell wall peptidoglycan synthesis in Escherichia coli K-12. Biochemical and Biophysical Research Communications 36, 682689.CrossRefGoogle ScholarPubMed
Neidhardt, F. C. (1966). Roles of amino acid activating enzymes in cellular physiology. Bacteriological Reviews 30, 701718.Google ScholarPubMed
Okada, T., Yanagisawa, K. & Ryan, F. J. (1960). Elective production of thymineless mutants. Nature, London 188, 340341.CrossRefGoogle Scholar
Rosso, G., Takashima, K. & Adams, E. (1969). Coenzyme content of purified alanine race-mase from Pseudomonas. Biochemical and Biophysical Research Communications 34, 134140.CrossRefGoogle Scholar
Strominger, J. L. (1962). Biosynthesis of bacterial cell walls. In Gunsalus, I. C. and Stanier, R. Y. (eds.), The Bacteria. Vol. III. Biosynthesis, pp. 413470. New York and London: Academic Press.Google Scholar
Taylor, A. L. (1970). Current linkage map of Escherichia coli. Bacteriological Reviews 34, 155175.Google ScholarPubMed
Verhoef, C. & de Haan, P. G. (1966). Genetic recombination in Escherichia coli. I. Relation between linkage of unselected markers and map distance. Mutation Research 3, 101110.CrossRefGoogle ScholarPubMed
Waddell, W. J. (1956). A simple ultraviolet spectrophotometric method for the determination of protein. Journal of Laboratory and Clinical Medicine 48, 311314.Google ScholarPubMed
Wijsman, H. J. W. (1970). Een genetische studie over de celwandsynthese bij Escherichia coli. Ph.D. Thesis, University of Amsterdam.Google Scholar
Wijsman, H. J. W. (1972). A genetic map of several mutations affecting the mucopeptide layer of Escherichia coli. Genetical Research Cambridge 20, 6574.CrossRefGoogle ScholarPubMed
Yaniv, M. & Gros, F. (1969). Studies on valyl-tRNA synthetase and tRNAval from Escherichia coli. III. Valyl-tRNA synthetases from thermosensitive mutants of Escherichia coli. Journal of Molecular Biology 44, 3145.CrossRefGoogle Scholar