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A genetic variant in the microRNA-146a gene is associated with susceptibility to alcohol use disorders

Published online by Cambridge University Press:  15 April 2020

I. Novo-Veleiro ,
R. González-Sarmiento ,
C. Cieza-Borrella ,
I. Pastor ,
F.-J. Laso  and
M. Marcos
I. Novo-Veleiro
Affiliation:
Alcoholism Unit, Department of Internal Medicine, University Hospital of Salamanca, Salamanca, Spain
R. González-Sarmiento
Affiliation:
Molecular Medicine Unit, Department of Medicine, University of Salamanca, Salamanca, Spain Institute of Biomedical Research of Salamanca (IBSAL), Salamanca, Spain
C. Cieza-Borrella
Affiliation:
Molecular Medicine Unit, Department of Medicine, University of Salamanca, Salamanca, Spain Institute of Biomedical Research of Salamanca (IBSAL), Salamanca, Spain
I. Pastor
Affiliation:
Alcoholism Unit, Department of Internal Medicine, University Hospital of Salamanca, Salamanca, Spain Molecular Medicine Unit, Department of Medicine, University of Salamanca, Salamanca, Spain Institute of Biomedical Research of Salamanca (IBSAL), Salamanca, Spain
F.-J. Laso
Affiliation:
Alcoholism Unit, Department of Internal Medicine, University Hospital of Salamanca, Salamanca, Spain Institute of Biomedical Research of Salamanca (IBSAL), Salamanca, Spain
M. Marcos*
Affiliation:
Alcoholism Unit, Department of Internal Medicine, University Hospital of Salamanca, Salamanca, Spain Molecular Medicine Unit, Department of Medicine, University of Salamanca, Salamanca, Spain Institute of Biomedical Research of Salamanca (IBSAL), Salamanca, Spain
*
*Corresponding author. Department of Internal Medicine, University Hospital of Salamanca, P° San Vicente, 58-156, Salamanca 37007, Spain. Tel.: +34 923291100x437; fax: +34 923294739. E-mail addresses: mmarcos@usal.es (M. Marcos).
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Abstract

Background:

Polymorphisms in the microRNA (miRNA) regulatory pathways are novel functional genetic variants whose association with alcoholism susceptibility has not been previously studied. Given the potential relationship between certain miRNAs and alcohol use disorders (AUDs), this study was designed to explore the association between two polymorphisms within hsa-miR-146a and hsa-miR-196a2 genes and susceptibility to these diseases.

Methods:

Three hundred and one male patients with AUDs and 156 sex-matched healthy volunteers were enrolled. Polymorphisms were genotyped using TaqMan® PCR assays. Allele and genotype frequencies were compared between groups and logistic regression analysis was also performed to analyze the model of inheritance.

Results:

There was a significantly higher prevalence of allele C carriers (47.8%) of the miR-146a G>C polymorphism (rs2910164) among patients with AUDs when compared with controls (35.9%), and multivariable logistic regression analysis showed that the C allele was associated with these AUDs (OR = 1.615, 95% CI 1.067–2.442; P = 0.023). Neither the genotype nor the allele distribution of miR-196a2 polymorphism (rs11614913) was significantly different between groups.

Conclusions:

This is the first genetic association study to explore the relationship of miRNA polymorphisms with AUDs and to show an association of the miR-146a C>G rs2910164 allelic variant with this disease.

Keywords

Genetic polymorphismsMicroRNAAlcoholism
Type
Original articles
Information
European Psychiatry , Volume 29 , Issue 5 , June 2014 , pp. 288 - 292
Copyright
Copyright © Elsevier Masson SAS 2014

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Footnotes

1

M. Marcos and F.-J. Laso are both senior authors of this paper.

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