Hostname: page-component-848d4c4894-nmvwc Total loading time: 0 Render date: 2024-06-28T22:01:47.062Z Has data issue: false hasContentIssue false
  • English
  • Français

Specific IgE response in patients with brucellosis

Published online by Cambridge University Press:  15 May 2009

G. F. Araj ,
A. R. Lulu ,
M. I. Khateeb  and
M. Haj
G. F. Araj
Affiliation:
Department of Microbiology, Faculty of Medicine, Kuwait University, P.O. Box 24923Kuwait13110
A. R. Lulu
Affiliation:
Department of Medicine, Jahra Hospital, MPH, Jahra, Kuwait
M. I. Khateeb
Affiliation:
Department of Medicine, Jahra Hospital, MPH, Jahra, Kuwait
M. Haj
Affiliation:
Department of Microbiology, Faculty of Medicine, Kuwait University, P.O. Box 24923Kuwait13110
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

In the search to find discriminative serological markers to differentiate between patients with acute brucellosis and those with chronic brucellosis, an enzyme-linked immunosorbent assay (ELISA) was used to determine and compare the brucella-specific IgE response in 80 sera from patients with acute brucellosis, 37 sera from patients with chronic brucellosis, 26 sera from patients with positive blood cultures for bacteria other than brucella and 51 sera from healthy controls. The IgE findings were compared to brucella-specific IgG, IgM, IgA and IgG1–4 demonstrated by ELISA, and to microagglutination test (MAT) results. Elevated (positive) antibrucella IgE titres were detected in 89 and 81 % of sera from patients with acute and chronic brucellosis respectively. The predominant antibodies found in patients with acute brucellosis were of the IgG, IgM, IgA, IgE, IgG1 and IgG3 types while in chronic brucellosis IgG, IgA, IgE and IgG4 were found. Although IgE can be detected in patients with brucellosis, it does not discriminate between the acute and chronic stages of the disease.

Type
Research Article
Information
Epidemiology & Infection , Volume 105 , Issue 3 , December 1990 , pp. 571 - 577
Copyright
Copyright © Cambridge University Press 1990

References

REFERENCES

1.Parratt, D, Nielsen, KH, White, RG, Payne, DJH. Radioimmunoassay of IgM, IgG and IgA brucella antibodies. Lancet 1977; i: 1075–8.CrossRefGoogle Scholar
2.Magee, JT. An enzyme-labelled immunosorbent assay for Brucella abortus antibodies. J Med Microbiol 1980; 13: 167–72.CrossRefGoogle ScholarPubMed
3.Sippel, JE, El-Masry, NA, Farid, Z. Diagnosis of human brucellosis with ELISA. Lancet 1982; ii: 19–21.CrossRefGoogle Scholar
4.De Klerk, E, Anderson, R. Comparative evaluation of the enzyme-linked immunosorbent assay in the laboratory diagnosis of brucellosis. J Clin Microbiol 1985; 21: 381–6.CrossRefGoogle ScholarPubMed
5.Araj, GF, Lulu, AR, Khateeb, MI, Saadah, MA, Shakir, RA. ELISA versus routine tests in the diagnosis of patients with systemic and neurobrucellosis. Acta Path Microbiol Immunol Scand 1988; 96: 171–6.CrossRefGoogle ScholarPubMed
6.Reddin, JL, Anderson, RK, Jennes, R, Spink, WW. Significance of 7S and macroglobulin Brucella agglutinins in human brucellosis. N Engl J Med 1965; 272: 1263–8.CrossRefGoogle ScholarPubMed
7.Coghlan, JD, Weir, DM. Antibodies in human brucellosis. Br Med J 1967; 2: 269–71.CrossRefGoogle ScholarPubMed
8.White, G. Immunoglobulin profiles of the chronic antibody responses: discussion in relation to brucellosis infection. Postgrad Med J 1978; 54: 595–602.CrossRefGoogle Scholar
9.Araj, GF, Lulu, AR, Mustafa, MY, Khateeb, MI. Evaluation of ELISA in the diagnosis of acute and chronic brucellosis in human beings. J Hyg 1986; 97: 457–69.CrossRefGoogle ScholarPubMed
10.Serre, A, Bascoul, S, Vendrell, JP, Cannat, A. Human immune response to Brucella infection. Ann Inst Past Micro 1986; 138: 113–7.CrossRefGoogle Scholar
11.Pellicer, T, Ariza, J, Foz, A, Pallares, R, Gudiol, F. Specific antibodies detected during relapse of human brucellosis. J Infect Dis 1988; 157: 918–24.CrossRefGoogle ScholarPubMed
12.Araj, GF. Profiles of brucella-specific immunoglobulin G subclasses in sera of patients with acute and chronic brucellosis. Serodiag Immunother Infect Dis 1988; 2: 401–10.CrossRefGoogle Scholar
13.Johansson, SGO, Bennich, HH, Berg, T. The clinical significance of IgE. Prog Clin Immunol 1972; 1: 157.Google ScholarPubMed
14.Jarrett, EEE, Miller, HRP. Production and activities of IgE in helminth infection. Prog Allergy 1982; 31: 178–233.Google ScholarPubMed
15.Tomizawa, S, Suzuki, S, Kuroume, T, Morikawa, A, Matsui, A, Fukasawa, T, Matsumura, T. Clinical significance of specific IgE antibody against streoptokinase-streptodornase in renal disease of children. Int Archs Allergy Appl Immun 1977; 54: 400–4.CrossRefGoogle Scholar
16.Nagel, JE, White, C, Lin, MS, Fireman, P. Comparison of tetanus and diphtheria IgE antibody in allergic and non allergic children. J Allergy Clin Immunol 1978; 63: 308–14.CrossRefGoogle Scholar
17.Schopfer, K, Baerlocher, K, Price, P. Staphylococcal IgE antibodies, hyperimmuno-globulinemia E and Staphylococcus aureus infection. N Engl J Med 1979; 300: 835–8.CrossRefGoogle Scholar
18.Pauri, K, Swe, T, Ramamoorth, YCL, Chodankar, VP. Immunoglobulin E in dengne haemorrhagic fever cases. Trans R Soc Trop Med Hyg 1979; 73: 451–2.Google Scholar
19.Salonen, E-M, Hori, T, Meurman, O, Vesikari, T, Vaheri, A. Kinetics of specific IgA, IgD, IgE, IgG and IgM antibody responses in rubella. J Med Virol 1985; 16: 1–9.CrossRefGoogle ScholarPubMed
20.VanLoon, AM, Vander, Logt JTM, Heessen, FWA, Vander, veen J. Quantitation of immunoglobulin E antibody to cytomegalovirus by antibody capture enzyme-linked immunosorbent assay. J Clin Microbiol 1985; 21: 558–61.CrossRefGoogle Scholar
21.Mathur, S, Goust, JM, Horger, EO, Fudenberg, HH. Immunoglobulin E anti-candida antibodies and candidiosis. Infect Immun 1977; 18: 257–9.CrossRefGoogle Scholar
22.Escande, A, Serre, A. IgE anti-brucella antibodies in the course of human brucellosis and after specific vaccination. Ints Archs Allergy Appl Immun 1982; 68: 172–5.CrossRefGoogle ScholarPubMed
23.Lulu, AR, Araj, GF, Khateeb, MI, Mustafa, MY, Yusuf, AR, Fenech, FF. Human brucellosis in Kuwait: a prospective study of 400 cases. Q J Med 1988; 66: 39–54.Google ScholarPubMed