Sampling considerations for herd-level measurement of faecal Escherichia coli antimicrobial resistance in finisher pigs

R. H. DUNLOP; S. A. McEWEN; A. H. MEEK; R. M. FRIENDSHIP; W. D. BLACK; R. C. CLARKE

doi:10.1017/S0950268899002411

Abstract

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The objective of this study was to determine the most efficient means of sampling faeces of finisher pigs for accurate and precise farm-level estimates of antimicrobial resistance among faecal Escherichia coli. Resistance to tetracycline and gentamicin of 8250 isolates of E. coli from 55 finisher pigs on one farm was measured with a hydrophobic grid membrane filter method. The between-pig, within-pen component of variance in resistance was large (97·5%), while between-pen, within-room and between-room components were small (2·5% and 0%, respectively). Using these resistance data, the abilities of two sampling strategies to estimate prevalence were modelled with a Monte Carlo ‘bootstrap’ procedure. Compositing faecal samples from several pigs before testing produced unbiased and precise estimates of prevalence and is simpler technically than individual animal testing.

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Sampling considerations for herd-level measurement of faecal Escherichia coli antimicrobial resistance in finisher pigs

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