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Salmonella isolation with Rappaport–Vassiliadis enrichment medium seeded with different sized inocula of pre-enrichment cultures of meat products and sewage polluted water

Published online by Cambridge University Press:  19 October 2009

P. Vassiliadis ,
Ch. Mavrommati ,
V. Kalapothaki ,
G. Chronas  and
M. Efstratiou
P. Vassiliadis
Affiliation:
The Hellenic Pasteur Institute, Athens 11521 and the Department of Hygiene and Epidemiology of the University of Athens, Goudi, Athens 11527, Greece
Ch. Mavrommati
Affiliation:
The Hellenic Pasteur Institute, Athens 11521 and the Department of Hygiene and Epidemiology of the University of Athens, Goudi, Athens 11527, Greece
V. Kalapothaki
Affiliation:
The Hellenic Pasteur Institute, Athens 11521 and the Department of Hygiene and Epidemiology of the University of Athens, Goudi, Athens 11527, Greece
G. Chronas
Affiliation:
The Hellenic Pasteur Institute, Athens 11521 and the Department of Hygiene and Epidemiology of the University of Athens, Goudi, Athens 11527, Greece
M. Efstratiou
Affiliation:
The Hellenic Pasteur Institute, Athens 11521 and the Department of Hygiene and Epidemiology of the University of Athens, Goudi, Athens 11527, Greece
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A total of 574 samples, of seven different types, were examined for the presence of salmonellas. All the specimens were pre-enriched in buffered peptone water and enriched in Rappaport–Vassiliadis medium (RV medium). In one trial 0·1 ml of pre-enrichment culture of 497 samples (79 chicken carcasses, 228 specimens of minced meat, 100 pork sausages, 19 samples of dried powdered chicken meat, 11 specimens of faeces of healthy pigs and 60 samples of sewage polluted natural sea water) was seeded to 10 ml as well as to 100 ml of RV medium. With the first inoculum (ratio 0·1:10), 111 samples were found to contain salmonellas, while with the second inoculum (ratio 0·1:100), only 102 positive samples were detected. This difference is marginally significant (P < 0·05). In another trial, 0·1 ml, 0·2 ml and 0·5 ml of pre-enrichment culture of 162 specimens (71 chicken carcasses, 40 samples of sewage polluted sea water and 51 samples of sewage polluted river water) were in turn introduced to 10 ml of RV medium. With the 0·1 ml inoculum 93 positive samples were detected, while with the 0·2 and 0·5 ml inocula 93 and 88 positive samples were found. The differences are not statistically significant. In these trials the growth of competing organisms was minimal with ratios of inocula 0·1:10 or 0·1:100.

Type
Research Article
Information
Journal of Hygiene , Volume 95 , Issue 1 , August 1985 , pp. 139 - 147
Copyright
Copyright © Cambridge University Press 1985

References

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