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Restriction enzyme analysis of faecal adenoviruses in Newcastle upon Tyne

Published online by Cambridge University Press:  15 May 2009

M. M. Willcocks ,
M. J. Carter ,
F. R. Laidler  and
C. R. Madeley
M. M. Willcocks
Affiliation:
Department of Virology, University of Newcastle upon Tyne and United Kingdom
M. J. Carter
Affiliation:
Department of Virology, University of Newcastle upon Tyne and United Kingdom
F. R. Laidler
Affiliation:
Royal Victoria Infirmary, Newcastle upon Tyne, United Kingdom.
C. R. Madeley
Affiliation:
Department of Virology, University of Newcastle upon Tyne and United Kingdom Royal Victoria Infirmary, Newcastle upon Tyne, United Kingdom.
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Adenovirus DNA was isolated directly from virus-containing stools and digested with restriction endonucleases. The resulting fragments were separated by polyacrylamide gel electrophoresis (PAGE) and visualized by silver staining. This enabled us to assign most of the viruses detected to subgenus, serotype and, sometimes, unique strains. Although less sensitive than electron microscopy, the method allowed more information about the infecting virus to be obtained and no cultivation was necessary. Comparison with culture also allowed dual infections to be recognized.

A 2-year survey of faecal adenoviruses in Newcastle upon Tyne showed that type 41 (strain 41a) was the predominant type and strain 41p was not recorded. Heterogeneity in strain 41a was also noted as found elsewhere. Adenovirus type 40 was common prior to 1985 but was absent during the last 2 years.

Type
Research Article
Information
Epidemiology & Infection , Volume 101 , Issue 2 , October 1988 , pp. 445 - 458
Copyright
Copyright © Cambridge University Press 1988

References

Adrian, Th., Wadell, G., Hierholzer, J. C. & Wigand, R. (1986). DNA restriction analysis of adenovirus prototypes 1 to 41. Archives of Virology 91, 277–290.CrossRefGoogle ScholarPubMed
Brandt, C. D., Kim, H. W., Yolken, R. H., Kapikian, A. Z., Arrobio, J. O., Rodriguez, W. J., Wyatt, R. O., Chanock, R. M. & Parrott, R. H. (1979). Comparative epidemiology of two rotavirus serotypes and other viral agents associated with pediatric gastroenteritis. American Journal of Epidemiology 110, 243–254.CrossRefGoogle ScholarPubMed
Brown, M., Petric, M. & Middleton, P. J. (1984a). Silver staining of DNA restriction fragments for the rapid identification of adenovirus isolates: application during nosocomial outbreaks. Journal of Virological Methods 9, 87–98.CrossRefGoogle ScholarPubMed
Brown, M., Petric, M. & Middleton, P. J. (1984b). Diagnosis of fastidious enteric adenoviruses 40 & 41 in stool specimens. Journal of Clinical Microbiology 20, 334–338.CrossRefGoogle ScholarPubMed
Buitenwerf, J., Louwerens, J. J. & De Jong, J. C. (1985). A simple and rapid method for typing adenoviruses 40 and 41 without cultivation. Journal of Virological Methods 10, 39–44.CrossRefGoogle ScholarPubMed
Flewett, T. H. (1976). Diagnosis of enteric virus. Proceedings of the Royal Society of Medicine 69, 693–696.CrossRefGoogle Scholar
Gardner, P. S. & McQutllin, J. (1980). Rapid Virus Diagnosis. Application of Immuno–fluorescence, 2nd ed.London: Butterworths.Google Scholar
Green, M., Mackey, J. K., Wold, W. S. M. & Rigden, P. (1979). Thirty one humanadenovirus serotypes (Ad1–Ad31) from five groups (A–E) based on DNA genome homologies. Virology 93, 481–492.CrossRefGoogle Scholar
Hammond, G., Hannan, C, Yeh, T., Fischer, K., Mauthe, G. & Straus, S. E. (1987). DNA hybridization for diagnosis of enteric adenovirus infection from directly spotted human faecal samples. Journal of Clinical Microbiology 25, 1881–1885.CrossRefGoogle Scholar
Herring, A. J., Inglis, N. F., Ojeh, C. K., Snodgrass, D. R. & Menzies, J. D. (1982). Rapid diagnosis of rotavirus infection by direct detection of viral nucleic acid in silver stained polyacrylamide gels. Journal of Clinical Microbiology 16, 473–477.CrossRefGoogle ScholarPubMed
De Jong, J. C., Wigand, R., Kidd, A. H., Wadell, G., Kapsenberg, J. G.Muzerie, C. J., Wermenbol, A. G. & Firtzlaff, R. G. (1983). Candidate adenovirus 40 and 41: fastidious adenoviruses from human infant stool. Journal of Medical Virology 11, 215–231.CrossRefGoogle ScholarPubMed
Kidd, A. H. (1983). Genome variants of adenovirus 41 (subgroup G) from children with diarrhoea in South Africa. Journal of Medical Virology 14, 49–59.CrossRefGoogle Scholar
Kidd, A. H., Banatvala, J. E. & De Jong, J. C. (1983). Antibodies to fastidious faecal adenoviruses (species 40 and 41) in sera from children. Journal of Medical Virology 11, 333–341.CrossRefGoogle ScholarPubMed
Laemmli, U. K. (1970). Cleavage of structural proteins during the assembly of bacteriophage T4. Nature 227, 680–685.CrossRefGoogle ScholarPubMed
Maniatis, T., Fritsch, E. F. & Sambrook, J. (1982). Molecular Cloning. A Laboratory Manual. Cold Spring Harbour Laboratory, USA.Google Scholar
Moosai, R. B., Carter, M. J. & Madeley, C. R. (1984). Rapid detection of enteric adenovirus and rotavirus: a simple method using polyacrylamide gel electrophoresis. Journal of Clinical Pathology 37, 1404–1408.CrossRefGoogle ScholarPubMed
Okayama, H. & Berg, P. (1982). High efficiency cloning of full length cDNA. Molecular Cell Biology 2, 161–170.Google ScholarPubMed
Richmond, S. J., Wood, D. J. & Bailey, A. S. (1988). Recent respiratory and enteric adenovirus infection in children in the Manchester area. Journal of the Royal Society of Medicine 81, 15–18.CrossRefGoogle ScholarPubMed
Rose, J. A., Hoggin, M. D., Koczot, F. J. & Shatkin, A. J. (1968). Genetic relatedness studies with adenovirus–associated virus. Journal of Virology 2, 999–1001.CrossRefGoogle Scholar
Schmitz, H., Wigand, R. & Heinrich, W. (1983). World–wide epidemiology of human adenovirus infections. American Journal of Epidemiology 117, 455–466.CrossRefGoogle Scholar
Takiff, H. E., Straus, S. E. & Garon, C. F. (1981). Propagation and in vitro studies of previously non–cultivable enteral adenoviruses in 293 cells. Lancet ii, 832–834.CrossRefGoogle Scholar
Takiff, H. E., Reinhold, W., Garon, C. F. & Straus, S. E. (1984). Cloning and physical mapping of enteric adenoviruses (candidate types 40 and 41). Journal of Virology 51, 131–136.CrossRefGoogle ScholarPubMed
Wadell, G. (1984). Molecular epidemiology of human adenoviruses. Current Topics in Microbiology and Immunology 110, 191–220.Google ScholarPubMed
Wadell, G. (1987). Adenoviruses. In Principles and Practice of Clinical Virology (ed. Zuckerman, A. J., Banatvala, J. E., Pattison, J. R.), pp. 251–274. Chichester: John Wiley & Sons.Google Scholar
Wadell, G., Cooney, M. K., Linhares, A. Da Costa, De Silva, L., Kennett, M. L., Kono, R., Gui-Fang, R., Lindman, K., Nascimento, J.P., Schoub, B.D. & Smith, C.D. (1985). Molecular epidemiology of adenoviruses: Global distribution of adenovirus 7 genome types. Journal of Clinical Microbiology 21, 403–408.CrossRefGoogle ScholarPubMed