Hostname: page-component-848d4c4894-wg55d Total loading time: 0 Render date: 2024-05-23T10:53:24.978Z Has data issue: false hasContentIssue false

R plasmids conferring multiple drug resistance from shigella isolated in Korea

Published online by Cambridge University Press:  19 October 2009

Doki Chun
Department of Microbiology, Keimyung University School of Medicine, Taegu, Republic of Korea
Dong Taek Cho
Department of Microbiology, Kyungpook National University School of Medicine, Taegu, Republic of Korea
Sung Yong Seol
Department of Microbiology, Kyungpook National University School of Medicine, Taegu, Republic of Korea
Min Ho Suh
Department of Microbiology, Kyungpook National University School of Medicine, Taegu, Republic of Korea
Yoo Chul Lee
Department of Microbiology, Kyungpook National University School of Medicine, Taegu, Republic of Korea
Rights & Permissions [Opens in a new window]


Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

The majority (85%) of shigella isolated in 1980 and 1981 in Korea were Shigella flexneri, the others were Sh. sonnei (14%) with only a small number of Sh. dysenteriae. Only 14 of the 459 strains of shigella isolated were susceptible to all 12 drugs tested, and 445 were resistant to three or more drugs. Strains multiply resistant to the six drugs, chloramphenicol (Cm), tetracycline (Tc), streptomycin (Sm), sulfisomidine (Su), ampicillin (Ap) and trimethoprim (Tp) were most frequently encountered, followed by those resistant to Cm, Tc, Sm, Su and Tp. The complete patterns of resistance to drugs except nalidixic acid and rifampin in approximately 73% of drug-resistant strains were co-transferred to Escherichia coli by conjugation, indicating that the resistance was R plasmid-mediated. Randomly selected R plasmids conferring various patterns of resistance markers were tested for the incompatibility groups, and almost all of them were classified into Inc FII. Two of three R plasmids conferring resistance to Cm, Tc, Sm and Su were classified into Inc B and one to Inc FII. Two R types with resistance markers of Cm, Tc, Sm and Ap were not classified with our standard plasmids used.

Research Article
Copyright © Cambridge University Press 1984



Anderson, E. S. (1965). Origin of transferable drug resistance factors in the Enterobacteriaceae. British Medical Journal ii, 12801291.Google Scholar
Barada, F. A. Jr, & Guerrant, R. L. (1980). Sulfamethoxazole-trimethoprim versusampicillin in treatment of acute invasive diarrhoea in adults. Antimicrobial Agents and Chemotherapy 17, 961964.CrossRefGoogle ScholarPubMed
Bochner, B. R., Huang, H., Schieven, G. L. & Ames, B. N. (1980). Positive selection for loss of tetracycline resistance. Journal of Bacteriology 143, 926933.CrossRefGoogle ScholarPubMed
Chun, D. & Seol, S. Y. (1978). Drug resistance and R plasmids of Salmonella and Shigella in Korea. Tropical Medicine 20, 123129.Google Scholar
Chun, D. & Seol, S. Y. (1979). Drug resistance of Shigell and Salmonella and the inhibition and elimination of drug resistance. [In Korean.] Journal of the Korean Society for Microbiology 14, 2737.Google Scholar
Chun, D., Seol, S. Y., Cho, D. T. & Tak, R. (1977). Drug resistance and R plasmid in Salmonella typhi isolated in Korea. Antimicrobial Agents and Chemotherapy 11, 209213.CrossRefGoogle Scholar
Chun, D., Seol, S. Y. & Suh, M. H. (1981). Transferable resistance to trimethoprim in Shigella. Journal of Infectious Diseases 143, 742.CrossRefGoogle ScholarPubMed
Datta, N. & Olarte, J. (1974). R factors in strains of Salmonella typhi and Shigella dysenteriae 1 isolated during epidemics in Mexico: classification by compatibility. Antimicrobial Agents and Chemotherapy 5, 310317.CrossRefGoogle ScholarPubMed
Edwards, P. R. & Ewing, W. H. (1972). Identification of Enterobacteriaceae, 3rd ed. Burgess Publishing Co., Minneapolis.Google Scholar
Frost, J. A. & Rowe, B.(1983). Plasmid-determined antibiotic resistance in Shigella flexneri isolated in England and Wales between 1974 and 1978. Journal of Hygiene 90, 2732.CrossRefGoogle ScholarPubMed
Grant, R. B., Bannatyne, R. M. & Shapley, A. J. (1976). Resist to chloramphenicol and ampicillin of Salmonella typhimurium in Ontario, Canada. Journal of Infectious Diseases 134, 254261.CrossRefGoogle ScholarPubMed
Ishiguro, N., Oka, C. & Sato, G.. (1978). Isolation of citrate-positive variants of Escherichia coli from domestic pigeons, pigs, cattle, and horses. Applied and Environmental Microbiology 36, 217222.CrossRefGoogle ScholarPubMed
Olarte, J., Filloy, L. & Galindo, E. (1976). Resistance of Shigella dysenteriae type 1 to ampicillin and other antimicrobial agents: isolated during a dysentery outbreak in a hospital in Mexico City. Journal of Infectious Diseases 133, 572575.CrossRefGoogle Scholar
Seol, S. Y. (1980). Types and antimicrobial drug resistance of Shigella isolated in Taegu area. [In Korean.] Kyungpook University Medical Journal 21, 527531.Google Scholar
Uhlin, B. E. & Nordstroem, K.. (1975). Plasmid incompatibility and control of replication: copy mutant of the R factor R1 in Escherichia coli K-12. Journal of Bacteriology 124, 641649.CrossRefGoogle Scholar
Watson, C. E. (1967). Infectious drug resistance in Shigella in Cape Town. South African Medical Journal 41, 728731.Google ScholarPubMed
Yoshikawa, M., Nagashima, S. & Mitsuhashi, S. (1971). Genetical distinction of R factors derived from shigellae and salmonellae. Japanese Journal of Microbiology 15, 425436.CrossRefGoogle ScholarPubMed