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Mycoplasmacidal activity of bovine milk for T-mycoplasmas

Published online by Cambridge University Press:  15 May 2009

J. Brownlie ,
C. J. Howard  and
R. N. Gourlay
J. Brownlie
Affiliation:
Agricultural Research Council, Institute for Research on Animal Diseases, Compton, Newbury, Berks. RG16 0NN
C. J. Howard
Affiliation:
Agricultural Research Council, Institute for Research on Animal Diseases, Compton, Newbury, Berks. RG16 0NN
R. N. Gourlay
Affiliation:
Agricultural Research Council, Institute for Research on Animal Diseases, Compton, Newbury, Berks. RG16 0NN
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Summary

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Normal bovine milk and whey was mycoplasmacidal for 6 of the 13 strains of bovine T-mycoplasmas examined. The in vitro assay used also demonstrated no killing of the human, canine and simian T-mycoplasma strains after 4 hr. incubation. However, there appeared to be some cow-to-cow variation in possession of this activity, and following E. coli endotoxin stimulation of the mammary gland the activity was considerably reduced.

Whey from three normal cows was fractionated on a Bio-Gel A 1·5 m. column and the mycoplasmacidal activity of the resulting five peaks assayed. Only the second peak, peak B, contained activity and was characterized as the only peak containing bovine IgA. The active component in whey, however, was found to be heat stable at 60° C. for 60 minutes and to pass through a dialysis membrane. This is inconsistent with it being immunoglobulin.

Type
Research Article
Information
Journal of Hygiene , Volume 73 , Issue 3 , December 1974 , pp. 415 - 423
Copyright
Copyright © Cambridge University Press 1974

References

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