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Molecular typing of Mannheimia (Pasteurella) haemolytica serotype A1 isolates from cattle in Japan

Published online by Cambridge University Press:  07 October 2003

K. KATSUDA ,
M. KOHMOTO ,
K. KAWASHIMA ,
H. TSUNEMITSU ,
T. TSUBOI  and
M. EGUCHI
K. KATSUDA
Affiliation:
Environmental Hygiene Section, Shichinohe Research Unit, National Institute of Animal Health, 31 Uminai, Shichinohe, Kamikita, Aomori 039-2586, Japan
M. KOHMOTO
Affiliation:
Pasturing Disease Section, Shichinohe Research Unit, National Institute of Animal Health, 31 Uminai, Shichinohe, Kamikita, Aomori 039-2586, Japan
K. KAWASHIMA
Affiliation:
Environmental Hygiene Section, Shichinohe Research Unit, National Institute of Animal Health, 31 Uminai, Shichinohe, Kamikita, Aomori 039-2586, Japan
H. TSUNEMITSU
Affiliation:
Environmental Hygiene Section, Shichinohe Research Unit, National Institute of Animal Health, 31 Uminai, Shichinohe, Kamikita, Aomori 039-2586, Japan
T. TSUBOI
Affiliation:
Clinical Epidemiology Section, National Institute of Animal Health, Tsukuba, Ibaraki 305-0856, Japan
M. EGUCHI
Affiliation:
Clinical Epidemiology Section, National Institute of Animal Health, Tsukuba, Ibaraki 305-0856, Japan
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Abstract

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Pulsed field gel electrophoresis (PFGE) and random amplified polymorphic DNA (RAPD) methods were applied for molecular typing of 130 Mannheimia (Pasteurella) haemolytica serotype A1 isolates obtained from 13 prefectures in Japan. These isolates were divided into 15 ApaI PFGE profiles that formed six distinct clusters (clusters A–F). Fifty-three (40·7%) isolates were classified in cluster B, and 20·0, 13·8, 12·3, 6·9 and 6·1% of isolates were in clusters E, A, F, D and C, respectively. The isolates of cluster B were differentiated into seven subtypes (B1–B7) and subtype B5 contained 63% (34/53) of isolates. RAPD revealed four banding patterns (types I–IV), and among 130 isolates 60·7% (79/130) of isolates were RAPD type I. All of the RAPD type I isolates were grouped into clusters A–C by PFGE. There was no relationship between molecular typing and geographic origin of these isolates. These results indicate that isolates of M. haemolytica A1 strain with various molecular profiles have already spread in Japan and may have caused sporadic infections.

Type
Research Article
Information
Epidemiology & Infection , Volume 131 , Issue 2 , October 2003 , pp. 939 - 946
Copyright
2003 Cambridge University Press