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Molecular epidemiology of Shigella flexneri in a diarrhoea-endemic area of Lima, Peru

Published online by Cambridge University Press:  26 February 2004

C. M. FERNANDEZ-PRADA ,
M. M. VENKATESAN ,
A. A. FRANCO ,
C. F. LANATA ,
R. B. SACK ,
A. B. HARTMAN  and
W. SPIRA
C. M. FERNANDEZ-PRADA
Affiliation:
Walter Reed Army Institute of Research (WRAIR), Silver Spring, MD 20910, USA
M. M. VENKATESAN
Affiliation:
Walter Reed Army Institute of Research (WRAIR), Silver Spring, MD 20910, USA
A. A. FRANCO
Affiliation:
Johns Hopkins University, Baltimore, MD 21205, USA
C. F. LANATA
Affiliation:
Instituto de Investigacion Nutricional, Lima, Peru
R. B. SACK
Affiliation:
Johns Hopkins University, Baltimore, MD 21205, USA
A. B. HARTMAN
Affiliation:
Walter Reed Army Institute of Research (WRAIR), Silver Spring, MD 20910, USA
W. SPIRA
Affiliation:
Johns Hopkins University, Baltimore, MD 21205, USA
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Abstract

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A year-long community-based study of diarrhoeal diseases was conducted in Canto Grande, a periurban community in Lima, Peru. In 109 (34%) houses out of 323 that were visited, at least one individual was detected with shigellosis. The frequency of the 161 shigella isolates obtained was as follows: 117 S. flexneri (73%), 21 S. boydii (13%), 15 S. dysenteriae (9%), and 8 S. sonnei (5%). Using a non-radioactive ipaH gene probe as a molecular epidemiological tool, a total of 41 S. flexneri strains were shown to be distributed in 25 intra-family comparisons by pairs (icp). Further subdivision, based on a comparison of the serotype, plasmid profile, antibiotic resistances and ipaH hybridization patterns indicated that Group I, with 11 icp (44%), had strains that were identical, Group II with 8 icp (32%), had strains that were different and Group III with 6 icp (24%), had strains with the same serotype and identical ipaH profiles but with differences in other markers. This data indicates that a diversity of shigella clones circulated in this community resulting from both clonal spread and horizontal transfer of genetic elements. Furthermore, ipaH profiling of isolates can be used not only to differentiate between closely related shigella strains but also with other parameters, help to understand the dynamics of the generation of new clones of pathogenic bacteria.

Type
Research Article
Information
Epidemiology & Infection , Volume 132 , Issue 2 , April 2004 , pp. 303 - 316
Copyright
© 2003 Cambridge University Press

Footnotes

The views expressed herein are those of the authors and do not necessarily represent those of the Department of the Army or the Department of Defense (para 4-3, AR 360-5).