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Molecular epidemiology and population genetics of Salmonella subspecies diarizonae in sheep in Norway and Sweden

Published online by Cambridge University Press:  26 February 2004

O. ALVSEIKE
Affiliation:
The Norwegian School of Veterinary Science, P.O. Box 8146 Dep, 0173 Oslo, Norway Norwegian Meat Research Centre, P.O. Box 396 Økern, 0513 Oslo, Norway
T. VARDUND
Affiliation:
National Institute of Public Health, P.O. Box 4404 Torshov, 0403 Oslo, Norway
B. LINDSTEDT
Affiliation:
National Institute of Public Health, P.O. Box 4404 Torshov, 0403 Oslo, Norway
E. HEIR
Affiliation:
National Institute of Public Health, P.O. Box 4404 Torshov, 0403 Oslo, Norway
E. ERIKSSON
Affiliation:
National Veterinary Institute, Department of Bacteriology, S-751 89 Uppsala, Sweden
G. KAPPERUD
Affiliation:
The Norwegian School of Veterinary Science, P.O. Box 8146 Dep, 0173 Oslo, Norway National Institute of Public Health, P.O. Box 4404 Torshov, 0403 Oslo, Norway
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Abstract

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Fifty-four isolates of Salmonella enterica subsp. diarizonae (IIIb) in Norway, Sweden, England, the United States, France and Australia were characterized by pulsed-field gel electrophoresis (PFGE). This study focuses on serovar 61:k:1,5,(7) [S. IIIb 61:k:1,5,(7)] isolated from sheep. Digestion of the bacterial DNA with restriction enzyme XbaI yielded 15 distinct PFGE profiles comprising 12–16 fragments in the range 48·5–630·5 kbp. Four different profiles were identified in Norwegian sheep isolates and a single profile in Swedish isolates. The spatial and temporal distribution of profiles is discussed.

Type
Research Article
Copyright
© 2003 Cambridge University Press