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Molecular characterization of trimethoprim resistance in Shigella sonnei in Sicily

Published online by Cambridge University Press:  15 May 2009

A. Agodi ,
C. Jones ,
E. J. Threlfall ,
M. D'Angelo  and
M. Marranzano
A. Agodi
Affiliation:
Institute of Hygiene and Preventive Medicine, University of Catania, via Biblioteca 4, 95100 Catania, Italy
C. Jones
Affiliation:
Public Health Laboratory Service, National Collection of Type Cultures, Central Public Health Laboratory, London
E. J. Threlfall
Affiliation:
Public Health Laboratory Service, Division of Enteric Pathogens, Central Public Health Laboratory, London
M. D'Angelo
Affiliation:
Institute of Hygiene and Preventive Medicine, University of Catania, via Biblioteca 4, 95100 Catania, Italy
M. Marranzano
Affiliation:
Institute of Hygiene and Preventive Medicine, University of Catania, via Biblioteca 4, 95100 Catania, Italy
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Summary

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During the 3-year period 1985–7, all strains of Shigella sonnei isolated in Catania, Sicily, showed a high level of resistance to trimethoprim (Tp) which was invariably associated with resistance to other antibiotics.

Plasmid analysis showed 18 different electropherotypes: 35 of 37 strains harboured a plasmid of 70 Megadaltons (MDa), and 29 of 37 strains a plasmid of 130 MDa.

Restriction endonuclease fingerprinting of purified 70 MDa plasmid DNA from different strains demonstrated that these plasmids were similar but not identical.

In some strains with transferable Tp resistance, DNA hybridization analysis demonstrated the presence of genes coding for the production of dihydrofolate reductase (DHFR) type V. In contrast, there was no detectable hybridization with DNA probes specific for genes coding for DHFR types I, II and IV. This is the first report of the DHFR type V gene outside Sri Lanka.

Type
Special Article
Information
Epidemiology & Infection , Volume 105 , Issue 1 , August 1990 , pp. 29 - 40
Copyright
Copyright © Cambridge University Press 1990

References

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