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The measurement of the D-antigen in poliovirus preparations

Published online by Cambridge University Press:  15 May 2009

A. J. Beale  and
P. J. Mason
A. J. Beale
Affiliation:
Virus Research Unit, Glaxo Laboratories Ltd, Sefton Park, Stoke Poges, Bucks
P. J. Mason
Affiliation:
Virus Research Unit, Glaxo Laboratories Ltd, Sefton Park, Stoke Poges, Bucks
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A simple micro-method of measuring ‘D-’ and ‘C-’ antigen contents of poliovirus preparations is described. The D-antigen of poliovirus is almost certainly the component of the virus involved in eliciting neutralizing antibodies, and the D-antigen assay thus becomes a simple in vitro method for assessing the antigenic potency of poliovaccines and for investigating the influence of various factors upon the antigen. The value of the technique in following the concentration and purification of poliovirus preparations is illustrated in the studies reported in the accompanying paper by Fantes (1962). The results of experiments designed to test the validity of the idea that D-antigen is the antigen eliciting neutralizing antibodies are discussed by Beale (1961).

Type
Research Article
Information
Journal of Hygiene , Volume 60 , Issue 1 , March 1962 , pp. 113 - 121
Copyright
Copyright © Cambridge University Press 1962

References

REFERENCES

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