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Low-temperature preservation of Leptospira, preliminary communication

Published online by Cambridge University Press:  15 May 2009

Joyce D. Coghlan ,
W. H. R. Lumsden  and
G. J. C. McNeillage
Joyce D. Coghlan
Affiliation:
Department of Bacteriology, University of Edinburgh Medical School
W. H. R. Lumsden
Affiliation:
Department of Bacteriology, University of Edinburgh Medical School
G. J. C. McNeillage
Affiliation:
Protozoology Research Unit, Royal (Dick) School of Veterinary Studies, University of Edinburgh
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1. A simple method for the long-term viable-preservation of samples of Leptospira suspensions is described. Quantities of about 25 μl., with the addition of 77·5 % glycerol, are introduced into capillary tubes, cooled slowly to −79° C. and stored at that temperature.

2. More than half the attempts were successful in re-establishing growth in culture after storage at −79° C. for periods up to 27 months.

3. The possibility that the method could be used for establishing ‘banks’ of standard Leptospira material for reference is discussed.

Type
Research Article
Information
Journal of Hygiene , Volume 65 , Issue 3 , September 1967 , pp. 373 - 379
Copyright
Copyright © Cambridge University Press 1967

References

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