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Legionella pneumophila serogroup 1 subgrouping by monoclonal antibodies – an epidemiological tool

Published online by Cambridge University Press:  19 October 2009

I. D. Watkins ,
J. O'H. Tobin ,
P. J. Dennis ,
W. Brown ,
R. Newnham  and
J. B. Kurtz
I. D. Watkins
Affiliation:
Sir William Dunn School of Pathology, South Parks Road, Oxford OX1 3RE
J. O'H. Tobin
Affiliation:
Sir William Dunn School of Pathology, South Parks Road, Oxford OX1 3RE
P. J. Dennis
Affiliation:
Centre for Applied Microbiology and Research, Porton Down, Salisbury, Wilts SP4 0JG
W. Brown
Affiliation:
Centre for Applied Microbiology and Research, Porton Down, Salisbury, Wilts SP4 0JG
R. Newnham
Affiliation:
Dept of Virology, John Radcliffe Hospital, Oxford OX3 9DU
J. B. Kurtz
Affiliation:
Dept of Virology, John Radcliffe Hospital, Oxford OX3 9DU
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Summary

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A panel of 10 monoclonal antibodies was used to subgroup 326 strains of Legionella pneumophila serogroup 1. All but two strains could be classified into three major subgroups named after their representative strains Pontiac 1, Olda and Bellingham 1. Of the 50 isolates from patients, 44 representing 32 separate incidents were of the Pontiac subgroup. This subgroup was also found in 16 of 18 buildings epidomiologically associated with Legionnaires' Disease. In contrast, strains of the Olda subgroup predominated in buildings where no infections had occurred. In 9 of the 11 incidents where isolates were available from at least one patient as well as from the suspected environmental source, the monoclonal antibody reaction patterns of strains from patients were identical to those of one or more of their environmental counterparts.

Type
Research Article
Information
Journal of Hygiene , Volume 95 , Issue 2 , October 1985 , pp. 211 - 216
Copyright
Copyright © Cambridge University Press 1985

References

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