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Influenza A and B virus IgG and IgM serology by enzyme immunoassays

Published online by Cambridge University Press:  19 October 2009

P. Koskinen ,
T. Vuorinen  and
O. Meurman
P. Koskinen
Affiliation:
Department of Virology, University of Turku, Kiinamyllynkatu 13, 20520 Turku, Finland.
T. Vuorinen
Affiliation:
Department of Virology, University of Turku, Kiinamyllynkatu 13, 20520 Turku, Finland.
O. Meurman
Affiliation:
Department of Virology, University of Turku, Kiinamyllynkatu 13, 20520 Turku, Finland.
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Summary

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Enzyme immunoassays (EIA) for IgG and IgM antibodies against influenza A and B virus are described. One hundred and seven subjects with a clinical diagnosis of acute respiratory infection (influenza, bronchitis or pneumonia) were selected for this study during two epidemics of influenza A which occurred in Finland in 1983 and 1985. Paired sera and nasopharyngeal secretions were obtained from all subjects. The sera were tested for influenza A and B antibodies by IgG and IgM EIAs and by complement fixation tests. The nasopharyngeal secretions were tested by an indirect EIA for influenza A and B antigens.

The IgG EIA was found to be better than complement fixation for the diagnosis of influenza A infections: only 22% of the significant increases detected by this test were also positive by complement fixation. The additional contribution of the IgM EIA to the number of positives was minimal.

It was also found that testing a single 1/1000 dilution of serum for influenza A and 1/100 dilution for influenza B in the IgG EIA gave as many positives as the conventional method of testing several dilutions.

Type
Research Article
Information
Epidemiology & Infection , Volume 99 , Issue 1 , August 1987 , pp. 55 - 64
Copyright
Copyright © Cambridge University Press 1987

References

REFERENCES

Bishai, F. R. & Galli, R. (1978). Enzyme-linked immunosorbent assay for detection of antibodies to influenza A and B and parainfluenza type 1 in sera of patients. Journal of Clinical Microbiology 8, 648–656.CrossRefGoogle Scholar
Chanock, R. M. (1979). Parainfluenza viruses. In Diagnostic Procedures for Viral, Rickettsial and Chlamydial Infections (ed. Lennette, K. H. and Smith, N. J.), pp. 611–632. Washington, D. C.: American Public Health Association.Google Scholar
de Savigny, D. & Voller, A. (1980). The communication of elisa data from laboratory to clinician. Journal of Immunoassay 1, 105–128.CrossRefGoogle ScholarPubMed
Hammond, G. W., Smith, S. J. & Noble, G. R. (1980). Sensitivity and specificity of enzyme immunoassay for serodiagnosis of influenza A virus infections. Journal of Infectious Diseases 141, 644–651.CrossRefGoogle ScholarPubMed
Julkunen, I., Klemola, M. & Hovi, T. (1984). Serological diagnosis of influenza A and B infections by enzyme immunoassay. Comparison with the complement fixation test. Journal of Virological Methods 9, 7–14.CrossRefGoogle Scholar
Julkunen, I., Pyhälä, R. & Hovi, T. (1985). Enzyme immunoassay, complement fixation and hemagglutination inhibition tests in the diagnosis of influenza A and B virus infections. Purified hemagglutinin in subtype specific diagnosis. Journal of Virological Methods 10. 75–84.CrossRefGoogle Scholar
Lambre, C. & Kasturi, K. N. (1979). A microplate immunoenzyme assay for anti-influenza antibodies. Journal of Immunological Methods 26, 61–67.CrossRefGoogle ScholarPubMed
Leinikki, P. & Pässilä, S. (1976). Solid phase antibody assay by means of enzyme conjugated to anti-immunoglobulin. Journal of Clinical Pathology 29, 1116–1120.CrossRefGoogle ScholarPubMed
Madore, H. P., Reichman, R. C. & Dolin, R. (1983). Serum antibody responses in naturally occurring influenza A virus infection determined by enzyme-linked immunosorbent assay, hemagglutination inhibition, and complement fixation. Journal of Clinical Microbiology 18, 1345–1350.CrossRefGoogle ScholarPubMed
Mashh, K. N. & Lange, W. (1980). Enzyme-linked immunosorbent assay for the detection of influenza type-specific antibodies. Journal of Immunological Methods 36, 173–179.Google Scholar
Sarkkinen, H. K., Halonen, P. E. & Salmi, A. A. (1981). Detection of influenza A virus by radioimmunoassay and enzymo-immunoassay from nasopharyngeal specimens. Journal of Medical Virology 7, 213–220.CrossRefGoogle Scholar