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The effect of timing of sample collection on the detection of measles-specific IgM in serum and oral fluid samples after primary measles vaccination

Published online by Cambridge University Press:  01 December 1999

R. F. HELFAND
Affiliation:
Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, 1600 Clifton Rd., Mailstop A-34, Atlanta, GA 30333
S. KEBEDE
Affiliation:
Department of Pediatrics, Addis Ababa University, Addis Ababa, Ethiopia
S. MERCADER
Affiliation:
Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, 1600 Clifton Rd., Mailstop A-34, Atlanta, GA 30333 Biokit, SA, Barcelona, Spain
H. E. GARY
Affiliation:
Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, 1600 Clifton Rd., Mailstop A-34, Atlanta, GA 30333
H. BEYENE
Affiliation:
Department of Pediatrics, Addis Ababa University, Addis Ababa, Ethiopia
W. J. BELLINI
Affiliation:
Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, 1600 Clifton Rd., Mailstop A-34, Atlanta, GA 30333
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Abstract

This study compares the timing of the rise and decline of measles-specific IgM in serum samples and in oral fluid samples. Two hundred and eighty 9-month-old infants presenting for routine measles vaccination in Addis Ababa, Ethiopia, were enrolled. Paired serum and oral fluid samples were collected before and 1, 2, 3 or 4 weeks after measles vaccination. Samples were tested by using a modified antibody-capture enzyme immunoassay. For the 321 IgM-negative pre- and post-vaccination serum samples, 317 (99%) of their corresponding oral fluid samples were IgM-negative. Among the 130 IgM-positive serum samples, 75% of their paired oral fluid samples were IgM-positive, with the percentage rising to 87% after oral fluid samples collected [ges ]3·5 weeks after vaccination were excluded. Among the post-vaccination serum samples, the percent IgM-positive peaked in week 3 and declined to 79% in week 4. For post-vaccination oral fluid samples, the percent IgM-positive peaked in weeks 2 and 3, and then declined to 43% in week 4. This modified antibody-capture enzyme immunoassay appears to detect vaccine-induced measles-specific IgM in the first 3 weeks after vaccination.

Type
Research Article
Copyright
© 1999 Cambridge University Press
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