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Comparison of clinical acinetobacter strains using a carbon source growth assay

Published online by Cambridge University Press:  15 May 2009

L. Dijkshoorn ,
A. Van Ooyen ,
W. C. J. Hop ,
M. Theuns  and
M. F. Michel
L. Dijkshoorn
Affiliation:
Department of Clinical Microbiology, University Hospital Rotterdam Dijkzigt, Dr. Molewaterplein 40, 3015 GD Rotterdam, The Netherlands
A. Van Ooyen
Affiliation:
Bioinformatica Group, University of Utrecht
W. C. J. Hop
Affiliation:
Department of Epidemiology and Biostatistics, Erasmus University of Rotterdam, The Netherlands
M. Theuns
Affiliation:
Department of Clinical Microbiology, University Hospital Rotterdam Dijkzigt, Dr. Molewaterplein 40, 3015 GD Rotterdam, The Netherlands
M. F. Michel
Affiliation:
Department of Clinical Microbiology, University Hospital Rotterdam Dijkzigt, Dr. Molewaterplein 40, 3015 GD Rotterdam, The Netherlands
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A quantitative carbon source growth assay, comprising ten carbon sources, was used to compare acinetobacter strains from three hospitals. The strains had been obtained during episodes of increased prevalence of isolations and were, for each hospital, assumed to be epidemiologically related. This assumption was supported by the electrophoretic protein profiles of the strains. Univariate analysis of growth data showed significant differences between strains from the three hospitals. Moreover, cluster analysis revealed that the major pattern in the data was related to the epidemiological origin of the strains. Exceptions to the epidemic-related pattern were observed. Thus, apart from epidemiological factors, other factors might contribute to carbon source growth profiles of the strains. It is concluded that the carbon growth assay may be useful to distinguish roughly between acinetobacter strains from different sites of origin. Further studies are required to analyse additional factors which influence carbon source growth of strains.

Type
Research Article
Information
Epidemiology & Infection , Volume 104 , Issue 3 , June 1990 , pp. 443 - 453
Copyright
Copyright © Cambridge University Press 1990

References

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