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A comparative investigation of the metabolism of the herbicide glufosinate in cell cultures of transgenic glufosinate-resistant and non-transgenic oilseed rape (Brassica napus) and corn (Zea mays)

Published online by Cambridge University Press:  15 October 2002

Monika Ruhland
Affiliation:
Bayerisches Landesamt für Gesundheit und Lebensmittelsicherheit, Außenstelle München, Menzinger Straße 54, 80638 München, Germany
Gabriele Engelhardt
Affiliation:
Bayerisches Landesamt für Gesundheit und Lebensmittelsicherheit, Außenstelle München, Menzinger Straße 54, 80638 München, Germany
Karlheinz Pawlizki
Affiliation:
Bayerische Landesanstalt für Bodenkultur und Pflanzenbau, Menzinger Straße 54, 80638 München, Germany

Abstract

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To obtain information on differences between the metabolic pathways of the herbicide glufosinate (trade names: BASTA®, LIBERTY®) in non-transgenic, glufosinate-sensitive plants and in transgenic, glufosinate-resistant plants, the metabolism of 14C-labeled glufosinate and its enantiomers L- and D-glufosinate was studied using cell cultures of oilseed rape and corn. Transformation of glufosinate in both sensitive and transgenic rape cells remained at a low rate of about 3-10% in contrast to corn cells, where 20% was transformed in sensitive and 43% in transgenic cells after 14 days of incubation, the rest remaining as unchanged glufosinate. In sensitive rape and corn cells the main metabolite was 4-methylphosphinico-2-oxo-butanoic acid (PPO) with 7.3 and 16.4%, respectively, together with low amounts of 3-methylphosphinicopropionic acid (MPP), 4-methylphosphinico-2-hydroxybutanoic acid (MHB), 4-methylphosphinicobutanoic acid (MPB) and 2-methylphosphinicoacetic acid (MPA). An additional metabolite formed in transgenic cell cultures was 2-acetamido-4-methylbutanoic acid (N-acetyl-L-glufosinate, NGA), which was formed at rates of 3.2% in rape and 16.1% in corn. A further minor metabolite, not yet identified, was detected in both cell types. The liberation of 0.2% 14CO2 indicates further metabolic steps prior to a limited mineralization in plant cell cultures. L-glufosinate was transformed into the same metabolites as the glufosinate racemate. D-glufosinate was not metabolized.

Type
Research Article
Copyright
© ISBR, EDP Sciences, 2002

References

Behrendt, H, Matthies, M, Gildemeister, H, Görlitz, G (1990) Leaching and transformation of glufosinate-ammonium and its main metabolite in a layered column. Environ. Toxicol. Chem. 9: 541-549 CrossRef
Dröge, W, Broer, I, Pühler, A (1992) Transgenic plants containing the phosphinothricin-N-acetyltransferase gene metabolize the herbicide L-phosphinothricin (glufosinate) differently from untransformed plants. Planta 187: 142-151 CrossRef
Dröge-Laser, W, Siemeling, U, Pühler, A, Broer, I (1994) The metabolites of the herbicide L-phosphinothricin (glufosinate) - identification, stability, and mobility in transgenic, herbicide-resistant, and untransformed plants. Plant Physiol. 105: 159-166 CrossRef
Gamborg, OL, Miller, RA, Ojima, K (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp. Cell Res. 50: 151-158 CrossRef
Jansen, C, Schuphan, I, Schmidt, B (2000) Glufosinate metabolism in excised shoots and leaves of twenty plant species. Weed Science 48: 319-326 CrossRef
Komoßa, D, Sandermann, H jr (1992) Plant metabolism of herbicides with C-P Bonds: phosphinothricin. Pestic. Biochem. Physiol. 43: 95-102 CrossRef
Langebartels, C, Harms, H (1986) Plant cell suspension cultures as test systems for an ecotoxicological evaluation of chemicals. Angew. Botanik 60: 113-123
Lea, PJ, Joy, KW, Ramos, JL, Guerrero, MG (1984) The action of the 2-amino-4-(methylphosphinyl)-butanoic acid (phosphinothricin) and its 2-oxo-derivative on the metabolism of cyanobacteria and higher plants. Phytochemistry 23: 1-6 CrossRef
Leason, M, Cunliffe, D, Parkin, D, Lea, PJ, Miflin, B (1982) Inhibition of pea leaf glutamine synthetase by methioninsulfoximine. Phosphinothricin and other glutamate analogs. J. Phytochem. 21: 855-857 CrossRef
Müller B, Schuphan I, Schmidt B (1999) Metabolismus von Glufosinate in Zellkulturen verschiedener Pflanzenspezies (transgen, nicht-transgen) - Probleme bei Versuchsdesign, Aufarbeitung und Analytik, Mitteilungen der Deutschen Phytomedizinischen Gesellschaft 29: 12-13
Müller, B, Zumdick, A, Schuphan, I, Schmidt, B (2001) Metabolism of the herbicide glufosinate-ammonium in plant cell cultures of transgenic (rhizomania-resistant) and non-transgenic sugar beet (Beta vulgaris), carrot (Daucus carota), purple foxglove (Digitalis purpurea) and thorn apple (Datura stramonium). Pest Manag. Sci. 57: 46-56 3.0.CO;2-1>CrossRef
Murashige, T, Skoog, F (1962) A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiol. Plant. 15: 473-477 CrossRef
Wohlleben W, Arnold W, Broer I, Hillemann D, Strauch E, Pühler A (1988) Nucleotide sequence of the phosphinothricin-N-acetyl-transferase gene from Streptomyces viridochromogenes Tü 494 and its expression in Nicotiana tabacum. Gene 70: 25-37 CrossRef
Zumdick A, Goerlitz G, Erzgräber B, Stumpf K, Dorn E (1998) Glufosinate-ammonium and N-acetyl-glufosinate: Soil metabolism. 9th Int. Congress of Pesticide Chemistry, London