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Increased copper levels inhibit denitrification in urban soils

Published online by Cambridge University Press:  06 December 2018

Shun LI ,
Xiaoru YANG ,
Daniel BUCHNER ,
Haitao WANG ,
Huijuan XU ,
Stefan B. HADERLEIN  and
Yongguan ZHU
Shun LI
Affiliation:
Key Lab of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, 1799 Jimei Road, Xiamen 361021, China. Email: xryang@iue.ac.cn Center for Excellence in Regional Atmospheric Environment, Institute of Urban Environment, Chinese Academy of Sciences, 1799 Jimei Road, Xiamen 361021, China. Center for Applied Geosciences, University of Tübingen, Hölderlinstr. 12, Tübingen 72074, Germany.
Xiaoru YANG*
Affiliation:
Key Lab of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, 1799 Jimei Road, Xiamen 361021, China. Email: xryang@iue.ac.cn Center for Excellence in Regional Atmospheric Environment, Institute of Urban Environment, Chinese Academy of Sciences, 1799 Jimei Road, Xiamen 361021, China.
Daniel BUCHNER
Affiliation:
Center for Applied Geosciences, University of Tübingen, Hölderlinstr. 12, Tübingen 72074, Germany.
Haitao WANG
Affiliation:
Key Lab of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, 1799 Jimei Road, Xiamen 361021, China. Email: xryang@iue.ac.cn
Huijuan XU
Affiliation:
Key Lab of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, 1799 Jimei Road, Xiamen 361021, China. Email: xryang@iue.ac.cn College of Natural Resources and Environment, South China Agricultural University, Guangzhou 510642, China.
Stefan B. HADERLEIN
Affiliation:
Center for Applied Geosciences, University of Tübingen, Hölderlinstr. 12, Tübingen 72074, Germany.
Yongguan ZHU
Affiliation:
Key Lab of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, 1799 Jimei Road, Xiamen 361021, China. Email: xryang@iue.ac.cn Center for Excellence in Regional Atmospheric Environment, Institute of Urban Environment, Chinese Academy of Sciences, 1799 Jimei Road, Xiamen 361021, China.
*
*Corresponding author
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Abstract

The consequences of urbanisation for Earth's biogeochemical cycles are largely unexplored. Copper (Cu) in urban soils is being accumulated mainly due to anthropogenic activities under rapid urbanisation. The increasing Cu concentrations may contribute to altering soil nitrogen (N) cycling in urban ecosystems through modulating denitrification processes. This research aims to identify how Cu impacts urban soil denitrification functions and denitrifier abundance. An urban park soil with a background total Cu concentration of 7.9μgg–1 was incubated anaerobically with different Cu amendments (10, 20, 40, 80 and 160μg Cu g–1 soil), similar to prevalent Cu contents in urban soils. We evaluated the soil denitrification functions using the acetylene (C2H2) inhibition method and assessed the denitrifier abundance by quantitative polymerase chain reaction (qPCR) analyses of denitrifying marker genes (nirK, nirS and nosZ). At the function level, we observed that both the potential soil denitrification activity and the N2O emission rate due to denitrification were significantly (P<0.05) inhibited by Cu; even the lowest Cu addition (10μg Cu g–1 soil) drastically affected the denitrification function. Moreover, Cu significantly (P<0.05) decreased the abundance of nirK and nirS genes at the additions of 160μg Cu g–1 soil and 40μg Cu g–1 soil, respectively, whereas it had no clear impact on nosZ gene copies. Further correlation analyses revealed that the potential denitrification activity was positively correlated to the copy numbers of nirK and nirS genes, but it was not correlated to nosZ gene abundance. These findings indicate that Cu additions inhibited soil denitrification function and decreased denitrifier abundance in the investigated urban park soil. Our results suggest that Cu accumulation in urban soils, resulting from urbanisation, may generally influence denitrification in urban ecosystems.

Keywords

N2OnirKnirSnosZurbanisation
Type
Articles
Information
Earth and Environmental Science Transactions of The Royal Society of Edinburgh , Volume 109 , Issue 3-4: Earth Surface Processes and Environmental Sustainability in China , September 2018 , pp. 421 - 427
Copyright
Copyright © The Royal Society of Edinburgh 2018 

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