Hostname: page-component-8448b6f56d-42gr6 Total loading time: 0 Render date: 2024-04-19T21:11:10.126Z Has data issue: false hasContentIssue false
  • English
  • Français

Regeneration of vgb-transgenic poplar (Populus alba×P. glandulosa) and the primary observation of growth

Published online by Cambridge University Press:  12 February 2007

Zhang Bing-Yu ,
Su Xiao-Hua ,
Li Yi-Liang ,
Huang Qin-Jun ,
Zhang Xiang-Hua  and
Zhang Lei
Zhang Bing-Yu
Affiliation:
Lab of Tree Breeding and Cultivation, State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China
Su Xiao-Hua*
Affiliation:
Lab of Tree Breeding and Cultivation, State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China
Li Yi-Liang
Affiliation:
Lab of Tree Breeding and Cultivation, State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China
Huang Qin-Jun
Affiliation:
Lab of Tree Breeding and Cultivation, State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China
Zhang Xiang-Hua
Affiliation:
Lab of Tree Breeding and Cultivation, State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China
Zhang Lei
Affiliation:
Lab of Tree Breeding and Cultivation, State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China
*
*Corresponding author: E-mail: suxh@caf.ac.cn
Get access Rights & Permissions [Opens in a new window]

Abstract

Increasing the growth rate is especially important for low-quality wood applications, so this has become an important goal in poplar breeding. The present study describes the transfer of Vitreoscilla haemoglobin (VHb) gene (vgb) driven by constitutive promoters, by Agrobacterium tumefaciens into poplar (Populus alba×P. glandulosa). From about 450 leaf discs used for transformation, 60 Kan-resistant plants were obtained, and 52 proved to be true transgenic plants. The transgenic nature of these plants was confirmed by polymerase chain reaction (PCR) amplification and Southern dot blot hybridization. The expression of vgb gene in transgenic plants was confirmed by reverse transcriptase-PCR (RT-PCR). The performance of the transgenic lines was evaluated during the first year of growth in a greenhouse. These plants showed no significant stable morphological differences from the untransformed plants. Among them, three vgb-transgenic lines exhibited noticeably higher growth rates in terms of height and diameter.

Keywords

growthP.alba×P. glandulosatransgenicsvgb
Type
Research Article
Information
Chinese Journal of Agricultural Biotechnology , Volume 3 , Issue 1 , April 2006 , pp. 59 - 64
Copyright
Copyright © China Agricultural University and Cambridge University Press 2006

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Confalonieri, M, Belenghi, B, Balestrazzi, A et al. , (2000) Transformation of elite white poplar (Populus alba L.) cv ‘Billafranca’ and evaluation of herbicide resistance. Plant Cell Reports 19: 978–982.CrossRefGoogle Scholar
Csaikl, UM, Bastian, H, Brettschneider, R, et al. (1998) Comparative analysis of different DNA extraction protocols: A fast, universal maxi-preparation of high quality plant DNA for genetic evaluation and phylogenetic studies. Plant Molecular Biology Reporter 61: 69–86.CrossRefGoogle Scholar
Delledonne, M, Allegro, G, Belenghi, B et al. , (2001) Transformation of white poplar (Populus alba L.) with a novel Arabidopsis thaliana cysteine proteinase inhibitor gene and analysis of insect pest resistance. Molecular Breeding 7: 35–42.CrossRefGoogle Scholar
Eriksson, M, Israelsson, M, Olsson, O and Moritz, T (2000) Increased gibberellin biosynthesis in transgenic trees promotes growth, biomass production and xylem fiber length. Nature Biotechnology 18: 784–788.CrossRefGoogle ScholarPubMed
Fladung, M, Kumar, S and Ahuja, MR (1997) Genetic transformation of Populus genotypes with different chimeric gene constructs: transformation efficiency and molecular analysis. Transgenic Research 6: 111–121.CrossRefGoogle Scholar
Franke, R, McMichael, CM, Meyer, K, Shirley, AM, Cusumano, JC and Chapple, C (2000) Modified lignin in tobacco and poplar plants overexpressing the Arabidopsis gene encoding ferulate 5-hydroxylase. Plant Journal 22: 223–234.CrossRefGoogle ScholarPubMed
Fu, J, Sampalo, R, Gallardo, F, Canovas, FM and Kirby, EG (2003) Assembly of a cytosolic pine glutamine synthetase holoenzyme in leaves of transgenic poplar leads to enhanced vegetative growth in young plants. Plant Cell and Environment 26: 411–418.CrossRefGoogle Scholar
Gallardo, F, Fu, J, Canton, FR, Garacia-Gutierrez, A, Canovas, FM and Kirby, EG (1999) Expression of a conifer glutamine synthetase gene in transgenic poplar. Planta 210: 19–26.CrossRefGoogle ScholarPubMed
Hohenstein, WG and Wright, LL (1994) Biomass energy production in the United States: an overview. Biomass Bioenergy 6: 161–173.CrossRefGoogle Scholar
Holmberg, N, Lilius, G, Bailey, JE and Bulow, L (1997) Transgenic tobacco expressing Vitreoscilla hemoglobin exhibits enhanced growth and altered metabolite production. Nature Biotechnology 15: 244–247.CrossRefGoogle ScholarPubMed
Kawaoka, A, Matsunaga, E, Endo, S, Kondo, S and Yoshida, K (2003) Ectopic expression of a horseradish peroxidase enhances growth rate and increases oxidative stress resistance in hybrid aspen. Plant Physiology 132: 1177–1185.CrossRefGoogle ScholarPubMed
Liang, H, Maynard, CA, Allen, RD and Powell, WA (2001) Increased Septoria musiva resistance in transgenic poplar leaves expressing a wheat oxalate oxidase gene. Plant Molecular Biology 45: 619–629.CrossRefGoogle ScholarPubMed
Mao, Z, Hu, Y, Zhong, J, Wang, L, Guo, J and Lin, Z (2003) Improvement of hydroponic growth and water tolerance of Petunias by the introduction of vhb gene. Acta Botanica Sinica 45: 205–210.Google Scholar
Olsen, JE, Junttila, O, Nilsen, J et al. , (1997) Ectopic expression of oat phytochrome A in hybrid aspen changes critical daylength for growth and prevents cold acclimatization. Plant Journal 12: 1339–1350.CrossRefGoogle Scholar
Rottmann, WH, Meilan, R, Sheppard, LA et al. , (2000) Diverse effects of overexpression of LEAFY and PTLE, a poplar (Populus) homolog of LEAFY/FLORICAULA, in transgenic poplar and Arabidopsis. Plant Journal 22: 235–245.CrossRefGoogle Scholar
Sambrook, H, Fritsch, EF and Maniatis, T (1989) Molecular Cloning: A Laboratory Manual, Vol. 2. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.Google Scholar
Strohm, M, Eiblmeier, M, Langebartels, C et al. , (1999) Responses of transgenic poplar (Populus tremula × P. alba) over-expressing glutathione synthetase or glutathione reductase to acute ozone stress: visible injury and leaf gas exchange. Journal of Experimental Botany 50: 365–374.CrossRefGoogle Scholar
Tuominen, H, Sitbon, F, Jacobsson, C, Sandberg, G, Olsson, O and Sundberg, B (1995) Altered growth and wood characteristics in transgenic hybrid aspen expressing Agrobacterium tumefaciens T-DNA indoleacetic acid-biosynthetic genes. Plant Physiology 109: 1179–1189.CrossRefGoogle ScholarPubMed
Tzfira, T, Vainstein, A and Altman, A. (1999) rol -Gene expression in transgenic aspen (Populus tremula) plants results in accelerated growth and improved stem production index. Trees 14: 49–54.Google Scholar
Yang, BH (2001) The newest fast-growing poplar variety — 84K. Exploitation of Agricultural Products 2: 31.Google Scholar