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RAPD analysis on genetic diversity of typical tea populations in Hunan Province

Published online by Cambridge University Press:  27 June 2008

Shen Cheng-Wen ,
Huang Yi-Huan ,
Huang Jian-An ,
Luo Jun-Wu ,
Liu Chun-Lin  and
Liu De-Hua
Shen Cheng-Wen
Affiliation:
Key Lab of Tea Science of China Ministry of Education, Changsha 410128, China College of Horticulture and Gardening of Hunan Agricultural University, Changsha 410128, China
Huang Yi-Huan*
Affiliation:
Key Lab of Tea Science of China Ministry of Education, Changsha 410128, China
Huang Jian-An
Affiliation:
Key Lab of Tea Science of China Ministry of Education, Changsha 410128, China
Luo Jun-Wu
Affiliation:
Key Lab of Tea Science of China Ministry of Education, Changsha 410128, China College of Horticulture and Gardening of Hunan Agricultural University, Changsha 410128, China
Liu Chun-Lin
Affiliation:
Crop Gene Engineering Key Laboratory of Hunan Province, Changsha 410128, China
Liu De-Hua
Affiliation:
College of Horticulture and Gardening of Hunan Agricultural University, Changsha 410128, China
*
*Corresponding author. E-mail: scw69@163.com
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Abstract

Genetic diversity and genetic variation of 240 adult plants of four tea populations in Hunan – Camellia sinensis var. sinensis, C. sinensis var. assamica cv. Duntsa, C. ptilophylla and C. sinensis var. assamica cv. Jianghua – were studied by rapid amplification of polymorphic DNA (RAPD) markers. The results showed 226 loci using 21 random primers (10 bp), of which 201 (88.9%) were polymorphic. The genetic diversity analysis indicated that Shannon's index was 0.43; 74.7% of which was within-population genetic diversity while 25.3% was among-population variation. The gene diversity indexes of total populations (HT), within populations (HS) and among populations (HST) were, respectively, 0.37, 0.28 and 0.09. The coefficient of gene differentiation (GST) among populations was 0.23, indicating a 76.7% variation within populations and 23.3% among populations. These results displayed a rich within-population genetic variation, as in Shannon's diversity index. Interpopulation gene flow (Nm) was 0.74, which indicates the limited genetic exchange between populations.

Keywords

Camellia sinensisRAPD analysispopulationgenetic diversity
Type
Research Papers
Information
Chinese Journal of Agricultural Biotechnology , Volume 5 , Issue 1 , April 2008 , pp. 67 - 72
Copyright
Copyright © China Agricultural University 2008

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Footnotes

First published in Journal of Agricultural Biotechnology 2007, 15(5): 855–860

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