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Genetic diversity of male sterile and low fertility germplasm of Citrus revealed using SSR markers

Published online by Cambridge University Press:  02 August 2007

Cao Qing-Qin ,
Meng Hai-Jun ,
Wen Xiao-Peng ,
Yi Hua-Lin  and
Deng Xiu-Xin
Cao Qing-Qin
Affiliation:
National Key Laboratory of Crop Genetic Improvement, Huazhong Agriculture University, Wuhan 430070, China
Meng Hai-Jun
Affiliation:
National Key Laboratory of Crop Genetic Improvement, Huazhong Agriculture University, Wuhan 430070, China
Wen Xiao-Peng
Affiliation:
National Key Laboratory of Crop Genetic Improvement, Huazhong Agriculture University, Wuhan 430070, China
Yi Hua-Lin
Affiliation:
National Key Laboratory of Crop Genetic Improvement, Huazhong Agriculture University, Wuhan 430070, China
Deng Xiu-Xin*
Affiliation:
National Key Laboratory of Crop Genetic Improvement, Huazhong Agriculture University, Wuhan 430070, China
*
*Corresponding author. E-mail: xxdeng@mail.hzau.edu.cn
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Abstract

The genetic diversity of 43 male sterile and low fertility Citrus accessions, as well as 13 fertile ones, were assessed using simple sequence repeat markers (SSRs). Thirty-five polymorphic alleles were generated from eight primers (on average 4.4 alleles per primer). Cluster analysis was performed via unweighted pair group method analysis (UPGMA) using the NTSYS-pc version 2.10. The results showed that the accessions could be classified into three groups: cultivars of mandarin were classified into group 1; those of sweet orange, grapefruit, ponkan or tangor into group 2; and Microcitrus with male sterile cytoplasm into group 3. Cluster analysis also revealed that Satsuma mandarin was more closely related to Bendiguangju mandarin than to Zaoju, Mankieh or Huangyan Bendizao tangerine. The present study on genetic diversity of male sterile and low fertility Citrus will provide useful information for further collection, preservation and utilization of this plant.

Keywords

Citrusmale sterilesimple sequence repeat markergenetic diversity
Type
Research Article
Information
Chinese Journal of Agricultural Biotechnology , Volume 4 , Issue 2 , August 2007 , pp. 99 - 104
Copyright
Copyright © China Agricultural University and Cambridge University Press 2007

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Footnotes

First published in Journal of Agricultural Biotechnology 2006, 14(6): 937–941

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