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Expression of vip2A(c) gene from Bacillus thuringiensis in insect cells

Published online by Cambridge University Press:  20 March 2007

Shi Yong-Xia ,
Lv Lei ,
Xu Wei ,
Yuan Mei-Jin  and
Pang Yi
Shi Yong-Xia
Affiliation:
State Key Laboratory for Biocontrol, Zhongshan (Sun Yat-sen) University, Guangzhou 510275, China Guangdong Inspection and Quarantine Technology Center, Guangzhou 510623, China
Lv Lei
Affiliation:
State Key Laboratory for Biocontrol, Zhongshan (Sun Yat-sen) University, Guangzhou 510275, China
Xu Wei
Affiliation:
State Key Laboratory for Biocontrol, Zhongshan (Sun Yat-sen) University, Guangzhou 510275, China
Yuan Mei-Jin
Affiliation:
State Key Laboratory for Biocontrol, Zhongshan (Sun Yat-sen) University, Guangzhou 510275, China
Pang Yi*
Affiliation:
State Key Laboratory for Biocontrol, Zhongshan (Sun Yat-sen) University, Guangzhou 510275, China
*
*Corresponding author. E-mail: ls12@zsu.edu.cn
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Abstract

Recombinant Bac-GV2 DNA was obtained by inserting a fused gfp gene with the Bacillus thuringiensis vip2A(c) gene encoding a possible enzymatic component under the control of the polyhedrin gene promoter of the baculovirus Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV). The Trichoplusia ni cell line TnHi5 was transfected with Bac-GFP and Bac-GV2 DNAs respectively. Fluorescent cells expressing the fusion protein GV2 were much fewer than those expressing green fluorescent protein (GFP) alone, and did not obviously increase in number from 2 to 5 days after transfection. This result showed that the Vip2A fusion protein might have an ADP-ribosylating activity on cell skeleton actin, exerting an influence on the production and diffusion of the budded virus from insect cells.

Keywords

Bacillus thuringiensisvip2A(c) geneADP-ribosylating activityAutographa californica multicapsid nucleopolyhedrovirus
Type
Research Article
Information
Chinese Journal of Agricultural Biotechnology , Volume 3 , Issue 3 , December 2006 , pp. 223 - 229
Copyright
China Agricultural University and Cambridge University Press 2006

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