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CRS-PCR polymorphisms of the GHR gene and its relationship with milk production traits in Chinese Holstein cows

Published online by Cambridge University Press:  29 January 2010

Wang Li-Juan ,
Li Qiu-Ling ,
Wang Chang-Fa ,
Wang Hong-Mei ,
Li Jian-Bin ,
Gao Yun-Dong ,
Hou Ming-Hai  and
Zhong Ji-Feng
Wang Li-Juan
Affiliation:
Dairy Cattle Research Centre, Shandong Academy of Agricultural Science, Jinan 250100, China College of Public Health, Shandong University, Jinan 250012, China
Li Qiu-Ling
Affiliation:
Dairy Cattle Research Centre, Shandong Academy of Agricultural Science, Jinan 250100, China
Wang Chang-Fa
Affiliation:
Dairy Cattle Research Centre, Shandong Academy of Agricultural Science, Jinan 250100, China
Wang Hong-Mei
Affiliation:
Dairy Cattle Research Centre, Shandong Academy of Agricultural Science, Jinan 250100, China
Li Jian-Bin
Affiliation:
Dairy Cattle Research Centre, Shandong Academy of Agricultural Science, Jinan 250100, China
Gao Yun-Dong
Affiliation:
Dairy Cattle Research Centre, Shandong Academy of Agricultural Science, Jinan 250100, China
Hou Ming-Hai
Affiliation:
Dairy Cattle Research Centre, Shandong Academy of Agricultural Science, Jinan 250100, China
Zhong Ji-Feng*
Affiliation:
Dairy Cattle Research Centre, Shandong Academy of Agricultural Science, Jinan 250100, China
*
*Corresponding author. E-mail: sdox2@163.com
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Abstract

Single-nucleotide polymorphisms (SNPs) of exon 8 of the GHR gene were detected in Chinese Holstein cows by polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP). The results showed that exon 8 of the GHR gene digested by TasI could be divided into two kinds of alleles and three kinds of genotypes. The frequencies of allele A and T were 0.6339 and 0.3661, respectively. The frequencies of genotypes AA, AT and TT were 0.459, 0.350 and 0.191, respectively. Sequencing showed one single nucleotide mutation T→A at 4962 bp of the gene in genotype TT when compared with genotype AA, and this mutation resulted in an amino acid change of phenylalanine (TTT)→tyrosine (TAT). The result of χ2 testing indicated that the genotypic frequency of the GHR gene digested by TasI did not fit with Hardy–Weinberg equilibrium in this population (P<0.05). The least-square mean of the fat percentage of this population was higher for genotype TT than for genotype AA (P<0.05).

Keywords

Chinese Holstein cattleGHR genemilk production traitsSNPsCRS-PCR
Type
Research Papers
Information
Chinese Journal of Agricultural Biotechnology , Volume 6 , Issue 3 , December 2009 , pp. 215 - 219
Copyright
Copyright © China Agricultural University 2009

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