Hostname: page-component-77c89778f8-sh8wx Total loading time: 0 Render date: 2024-07-24T10:33:00.575Z Has data issue: false hasContentIssue false

Relationship between genetic variations of 5′-upstream and the second intron region of H-FABP gene and IMF content in eight pig breeds and wild pig

Published online by Cambridge University Press:  12 February 2007

Pang Wei-Jun
Affiliation:
Laboratory of Animal Fat Deposition and Muscle Development, Northwest A&F University, Yangling, Shaanxi 712100, China
Yang Gong-She*
Affiliation:
Laboratory of Animal Fat Deposition and Muscle Development, Northwest A&F University, Yangling, Shaanxi 712100, China
*
*Corresponding author. Email: gongshe-yang@163.com

Abstract

The genetic variations of the 5′-upstream region and the second intron of the porcine H-FABP gene were investigated by polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP) in 256 pigs, including Duroc, Large White, Landrace, Neijiang, Rongchang, Bamei pig, Hanjiang Black, Hanzhong White, and wild pigs. The effect of the H-FABP gene on intramuscular fat (IMF) content was analysed by the least square method. Results showed a HinfI-RFLP in these eight pig breeds and wild pigs, among which Large White, Bamei pig, Hanjiang Black, Hanzhong White and wild pigs presented low polymorphism, while the other breeds had intermediate polymorphism. There were no HaeIII or MspI-RFLPs in the four Chinese local pig breeds tested, but Duroc, Landrace, Large White, Hanzhong White and wild pig displayed polymorphism. Landrace, Large White and wild pigs had low levels of HaeIII- and MspI-RFLP, while the others had intermediate polymorphism. H-FABP genotypes significantly affected IMF content (P<0.05). IMF content according to H-FABP genotypes were HH>Hh>hh, DD<Dd<dd, AA<Aa<aa. The genetic effect values were 3.95 (HH), 3.48 (Hh), 3.23 (hh), 2.33 (DD), 2.55 (Dd), 2.97 (dd), 2.34 (AA), 2.76 (Aa) and 3.01 (aa). Results suggest that porcine meat quality may be improved by increasing the frequency of genotype aa-dd-HH in pig breeds.

Type
Research Article
Copyright
Copyright © China Agricultural University and Cambridge University Press 2006

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Binas, B, Danneberg, H, McWhir, J, Mullins, L and Clark, JA (1999) Requirement for the heart-type fatty acid binding protein in cardiac fatty acid utilization. FASEB Journal 13: 805810.CrossRefGoogle ScholarPubMed
Cao, HH, Zhang, GX, Wang, LX, Li, HB and Zheng, YM (2002) Sequence analysis of polymorphic fragments of porcine H-FABP gene. Hereditas (Peking) 24: 146148.Google ScholarPubMed
Gerbens, F, Rettenberger, D, Lenstra, JA, Meuwissen, TH, Janss, LL and Groenen, MA (1997) Characterization, chromosomal localization and genetic variation of porcine heart fatty acid-binding protein gene. Mammalian Genome 8: 328332.CrossRefGoogle ScholarPubMed
Gerbens, F, van Erp, AJH, Harders, FL, et al. (1999) Effect of genetic variants of the heart fatty acid-binding protein gene on intramuscular fat and performance traits in pigs. Journal of Animal Science 77(4): 846852.CrossRefGoogle ScholarPubMed
Gerbens, F, de Koning, DJ, Harders, FL, et al. (2000) The effect of adipocyte and heart fatty acid-binding protein genes on intramuscular fat and backfat content in Meishan crossbred pigs. Journal of Animal Science 78: 552559.CrossRefGoogle ScholarPubMed
Gerbens, F, Verburg, FJ, Van Moerkerk, HT, et al. (2001) Associations of heart and adipocyte fatty acid-binding protein gene expression with intramuscular fat content in pigs. Journal of Animal Science 79: 347354.CrossRefGoogle ScholarPubMed
Grindflek, E, Szyda, J, Rothschild, M and Lien, SA (2000) QTL analysis and candidate gene study for meat quality on swine chromosome 6. In: Proceedings of the 8th Conference on the Plant and Animal Genome, San Diego, California, USA, pp. 151156.Google Scholar
Hovenier, R, Kanis, E, Van Asseldonk, T and Westerink, NG (1992) Genetic parameters of pig meat quality traits in a halothane negative population. Livestock Production Science 32: 309321.CrossRefGoogle Scholar
Hovenier, R, Kanis, E and Verhoeven, JA (1993a) Repeatability of taste panel tenderness scores and their relationships to objective pig meat quality traits. Journal of Animal Science 71: 20182025.CrossRefGoogle ScholarPubMed
Hovenier, R, Kanis, E, Van Asseldonk, T and Westerink, NG (1993b) Breeding for pig meat quality in halothane negative populations—a review. Pig News Information 14: 17N25N.Google Scholar
Janss, LLG, de Koning, DJ, Rattubk, P, Van Arendonk, JA and Brascamp, EW (1999) Detection of quantitative traits loci for backfat thickness and intramuscular fat content in pig. Genetics 152: 16791690.Google Scholar
Lin, WH, Huang, LS, Ren, J, et al. (2002) Research on genetic variation of heart fatty acid-binding protein gene in ten pig breeds. Acta Genetica Sinica 29: 1215.Google ScholarPubMed
Meuwissen, THE and Goddard, ME (1996) The use of marker haplotypes in animal breeding schemes. Genetics Selection Evolution 28: 161176.CrossRefGoogle Scholar
Sambrook, J, Fritsch, EF and Maniatis, T (1998) Molecular Cloning, A Laboratory Manual. 2nd ed. Peking: Science Press.Google Scholar
Schaap, FG (1998) Fatty acid-binding proteins in the heart. Molecular and Cellular Biochemistry 180: 4351.CrossRefGoogle ScholarPubMed
Veerkamp, JH and Maatman, RGHJ (1995) Cytoplasmic fatty acid binding proteins: their structure and genes. Progress in Lipid Research 34: 1752.CrossRefGoogle ScholarPubMed
Zhang, Y and Zhang, Q (1993) Line Model of Breeding in Animal and Poultry. Peking: Higher Education Press.Google Scholar