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Rat adipocyte apoptosis induced by TNF-α

Published online by Cambridge University Press:  12 February 2007

Lin Ya-Qiu
Affiliation:
Laboratory of Fat Deposition and Muscle Development, Northwest Sci-tech University of Agriculture and Forestry, Yangling 712100, China
Li Rui-Wen
Affiliation:
Laboratory of Fat Deposition and Muscle Development, Northwest Sci-tech University of Agriculture and Forestry, Yangling 712100, China
Sun Chao
Affiliation:
Laboratory of Fat Deposition and Muscle Development, Northwest Sci-tech University of Agriculture and Forestry, Yangling 712100, China
Chen Guo-Zhu
Affiliation:
Laboratory of Fat Deposition and Muscle Development, Northwest Sci-tech University of Agriculture and Forestry, Yangling 712100, China
Yang Yong-Qing
Affiliation:
Laboratory of Fat Deposition and Muscle Development, Northwest Sci-tech University of Agriculture and Forestry, Yangling 712100, China
Meng De-Lian
Affiliation:
Laboratory of Fat Deposition and Muscle Development, Northwest Sci-tech University of Agriculture and Forestry, Yangling 712100, China
Yang Gong-She*
Affiliation:
Laboratory of Fat Deposition and Muscle Development, Northwest Sci-tech University of Agriculture and Forestry, Yangling 712100, China
*
*Corresponding author: Email gsyang999@sohu.com

Abstract

The effects of different concentrations of tumour necrosis factor-α (TNF-α) on rat adipocyte apoptosis were detected by optical microscopy, agarose gel electrophoresis and flow cytometry methods. The morphological changes of rat adipocyte apoptosis induced by TNF-α correlated linearly with the concentration of TNF-α, ranging from 5 to 20 ng/ml. High concentrations of TNF-α induced more obvious apoptosis. Significant morphological changes of rat adipocytes treated with 5 ng/ml TNF-α were noticed, but DNA ladders did not appear in the DNA electrophoresis analysis, i.e. morphological changes occurred earlier than the biochemical changes. TNF-α induced apoptosis in the rat adipocyte in a dose-dependent manner. The induced apoptotic effect of 5, 10, 15 and 20 ng/ml TNF-α was significantly different (P0.01), but the effect among 10, 15 and 20 ng/ml TNF-α treatments was not significantly different (P0.05). Thus the optimum concentration of TNF-α for inducing apoptosis was 10 ng/ml.

Type
Research Article
Copyright
Copyright © China Agricultural University and Cambridge University Press 2005

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