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Genetic diversity of male sterile and low fertility germplasm of Citrus revealed using SSR markers

Published online by Cambridge University Press:  02 August 2007

Cao Qing-Qin
Affiliation:
National Key Laboratory of Crop Genetic Improvement, Huazhong Agriculture University, Wuhan 430070, China
Meng Hai-Jun
Affiliation:
National Key Laboratory of Crop Genetic Improvement, Huazhong Agriculture University, Wuhan 430070, China
Wen Xiao-Peng
Affiliation:
National Key Laboratory of Crop Genetic Improvement, Huazhong Agriculture University, Wuhan 430070, China
Yi Hua-Lin
Affiliation:
National Key Laboratory of Crop Genetic Improvement, Huazhong Agriculture University, Wuhan 430070, China
Deng Xiu-Xin*
Affiliation:
National Key Laboratory of Crop Genetic Improvement, Huazhong Agriculture University, Wuhan 430070, China
*
*Corresponding author. E-mail: xxdeng@mail.hzau.edu.cn

Abstract

The genetic diversity of 43 male sterile and low fertility Citrus accessions, as well as 13 fertile ones, were assessed using simple sequence repeat markers (SSRs). Thirty-five polymorphic alleles were generated from eight primers (on average 4.4 alleles per primer). Cluster analysis was performed via unweighted pair group method analysis (UPGMA) using the NTSYS-pc version 2.10. The results showed that the accessions could be classified into three groups: cultivars of mandarin were classified into group 1; those of sweet orange, grapefruit, ponkan or tangor into group 2; and Microcitrus with male sterile cytoplasm into group 3. Cluster analysis also revealed that Satsuma mandarin was more closely related to Bendiguangju mandarin than to Zaoju, Mankieh or Huangyan Bendizao tangerine. The present study on genetic diversity of male sterile and low fertility Citrus will provide useful information for further collection, preservation and utilization of this plant.

Type
Research Article
Copyright
Copyright © China Agricultural University and Cambridge University Press 2007

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Footnotes

First published in Journal of Agricultural Biotechnology 2006, 14(6): 937–941

References

Beckett, JB (1971) Classification of male-sterile cytoplasms in maize (Zea may L.). Crop Science 11: 724727.CrossRefGoogle Scholar
Besnard, G, Khadari, B and Bervillé, VP (2000) Cytoplasmic male sterility in the olive (Olea europaea L.). Theoretical and Applied Genetics 100: 10181024.CrossRefGoogle Scholar
Cheng, YJ, Yi, HL, Pang, XM, Guo, WW and Deng, XX (2001) An efficient method for genomic DNA extraction from woody fruit plants. Journal of Huazhong Agricultural University 20(5): 481483 (in Chinese with English abstract).Google Scholar
Corazza-Nues, MJ, Machado, MA, Nues, WMC, Cristofani, M and Targon, MLPN (2002) Assessment of genetic variability in grapefruits (Citrus papadisi Macf.) and pummelos (C. maxima (Burm.) Merr.) using RAPD and SSR markers. Euphytica 126(2): 169176.CrossRefGoogle Scholar
De Oliveira, AC, Novac, GA, Cristofani, M and Machado, MA (2002) Identification of Citrus hybrids through the combination of leaf apex morphology and SSR markers. Euphytica 128(3): 397403.CrossRefGoogle Scholar
Deng, XX, Guo, WW and Sun, XH (1996) Advances in breeding and selection of seedless types of Citrus in China. Acta Horticulturae Sinica 23: 235240 (in Chinese with English abstract).Google Scholar
Fang, DQ and Zhang, WC (1992) Studies on the taxonomy and evolution of mandarins in China by isozymes. Journal of Wuhan Botanical Research 10(4): 305312 (in Chinese with English abstract).Google Scholar
Fang, XJ, Wu, WR and Tang, JL (2001) Assistant Breeding of Crop with DNA Markers. Beijing: Science Press (in Chinese).Google Scholar
Gulsen, O and Roose, ML (2001) Lemons: diversity and relationships with selected Citrus genotypes as measured with nuclear genome markers. Journal of the American Society for Horticultural Science 126(3): 309317.CrossRefGoogle Scholar
Hu, CG (1998) Molecular evaluation of Citrus genetic diversity and study of original taxonomy. PhD thesis, Huazhong Agriculture University, Wuhan, China (in Chinese with English abstract).Google Scholar
Kijas, JMH, Fowler, JCS and Thomas, MR (1995) An evaluation of sequence-tagged microsatellite site markers for genetic analysis within Citrus and related species. Genome 38: 349355.CrossRefGoogle ScholarPubMed
Li, YT, Xiong, QE, Ma, JY, et al. (1991) A preliminary study on Meishan seedless Jincheng sweet orange. South China Fruits 20(1): 810 (in Chinese with English abstract).Google Scholar
Liu, JH, Pang, XM, Cheng, YJ, Meng, HJ and Deng, XX (2002) Molecular characterization of the nuclear and cytoplasmic genomes of intergeneric diploid plants from cell fusion between Microcitrus papuana and Rough lemon. Plant Cell Reports 21: 327332.CrossRefGoogle Scholar
Liu, SJ, Rong, TZ, Yang, JP and Pan, GT (2004) Cluster analysis of local maize (Zea mays L.). Acta Agronomica Sinica 30(3): 221226 (in Chinese with English abstract).Google Scholar
Meng, HJ, Cao, QQ, Hu, ZY, Liu, GP, Cheng, YJ and Deng, XX (2005) Analysis of SSR in Citrus sequences from EMBL database. Agricultural Sciences in China 4(7): 501506.Google Scholar
Omura, M, Hidaka, T, Nesumi, H, et al. (1993) PCR Markers for Citrus Identification and Mapping Techniques on Gene Diagnosis and Breeding in Fruit Trees. Japan: FTRs.Google Scholar
Pang, XM, Hu, CG and Deng, XX (2003) Phylogenetic relationships among Citrus and its relatives as revealed by SSR markers. Acta Genetica Sinica 30(1): 8187.Google ScholarPubMed
Shen, DX, Wang, YY and Chen, LG (1998) Genetic Breeding of Citrus. Beijing: Science Press (in Chinese).Google Scholar
Sneath, PHA and Soical, RR (1973) Numerical Taxonomy. San Francisco: W. H. Freeman.Google Scholar
Tang, SM and Hu, BH (2001) Selection of a seedless, good-quality and high-yield cultivar of Ponkan-Qianyang seedless ponkan. South China Fruits 30(5): 36 (in Chinese with English abstract).Google Scholar
Vardi, A, Arzee-Gonen, P, Frydman-Shani, A, Bldichman, S and Galum, E (1989) Protoplast-fusion-mediated transfer of organelles from Microcitrus into Citrus and regeneration of novel alloplasmic trees. Theoretical and Applied Genetics 78: 741747.CrossRefGoogle ScholarPubMed
Yamamoto, M and Kobayashi, S (1995) A cybrid plant produced by electrofusion between Citrus unshiu (satsuma mandarin) and C. sinensis (sweet orange). Plant Tissue Culture Letters 12(2): 131137.CrossRefGoogle Scholar