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Effect of insulin on transcription of lipogenic and lipolytic-related genes and lipid metabolism in primary porcine adipocytes

Published online by Cambridge University Press:  12 February 2007

Lu Jian-Xiong
Affiliation:
Laboratory of Animal Fat Deposition and Muscle Development, Northwest Sci-Tech University of Agriculture and Forestry, Yangling 712100, China College of Life Science and Engineering, Northwest University for Nationalities, Lanzhou 730030, China
Chen Fen-Fen
Affiliation:
Laboratory of Animal Fat Deposition and Muscle Development, Northwest Sci-Tech University of Agriculture and Forestry, Yangling 712100, China
Yang Gong-She*
Affiliation:
Laboratory of Animal Fat Deposition and Muscle Development, Northwest Sci-Tech University of Agriculture and Forestry, Yangling 712100, China
*
*Corresponding author. Email: gsyang999@yahoo.com.cn

Abstract

Primary adipocytes from subcutaneous adipose tissue samples obtained from 7-day-old Yorkshire×Landrace crossbreed piglets were exposed to 0–400 nmol/l of insulin for 48 h. The accumulated triglyceride was measured through Oil Red O staining and the cumulative glycerol released was determined to assess lipolytic activity in adipocytes. Transcription levels of sterol regulatory element binding protein (SREBP)-1c, carbohydrate response element binding protein (ChREBP), acetyl-CoA carboxylase 1 (ACC1), fatty acid synthase (FAS), and hormone-sensitive lipase (HSL) were assessed using reverse transcriptase-polymerase chain reaction (RT-PCR). The results showed that ChREBP and ACC1 mRNA levels were not influenced by insulin alone under low glucose (5 nmol/l). FAS mRNA level was markedly stimulated by all doses of insulin except 200 nmol/l, and SREBP-1c mRNA level increased with 100–300 nmol/l insulin. High insulin doses (300 and 400 nmol/l) increased the HSL mRNA level as well as lipolytic activity.

Type
Research Article
Copyright
Copyright © China Agricultural University and Cambridge University Press 2006

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